[Histonet] Help

2012-12-28 Thread Ly Nguyen
I want to be remove from the list. I've tried the unsubscribe link be low several time but it seem like it doesn't work because I'm still receiving daily email On Friday, December 28, 2012, wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To

Re: [Histonet] Help

2012-12-28 Thread Marvin Hanna
Hi Ly, When you click on the Unsubscribe button, an email is sent to you to confirm you want to unsubscribe. It has a link to click to confirm you want to unsubscribe. Is the confirmation email getting lost in your junk folder? You won't be unsubscribed until you click the link in the

RE: [Histonet] Help

2012-12-28 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
For some reason I just received 3 emails from Histonet asking me to confirm if I want to be unsubscribedI did not request to be unsubscribed. Did anyone else receive emails like this? Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch

[Histonet] CAP and negative IHC controls

2012-12-28 Thread jsjurczak
How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Help

2012-12-28 Thread Marvin Hanna
Hi Jeanine, Hmmm... The only way that should happen is if someone put your email address in and clicked unsubscribe 3 times. That's the purpose of the confirmation email so that no one else can unsubscribe (or subscribe) you. So, anyone out there trying to unsubscribe Jeanine, please stop.

RE: [Histonet] CAP and negative IHC controls

2012-12-28 Thread Debra Siena
It is my understanding that you still need a negative tissue control but if using a polymer detection kit that you may stop doing a negative reagent control. The negative tissue control is a tissue known to not contain the antigen in the tissue, it can be an internal control, or a separate

Re: [Histonet] CAP and negative IHC controls

2012-12-28 Thread Richard Cartun
That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a Negative Reagent Control for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent

[Histonet] Re: Histonet Digest, Vol 109, Issue 30

2012-12-28 Thread Madeleine Huey
Kimura, The answer to your question is YES. Both Tween 20 Triton 100 are non-ionic detergents. Tween 20 is not as strong/effective as Triton 100, but you can use higher percentage Tw20 or prolong the permeabilization timing. Best! Madeleine Huey, BS, MT, HTL QIHC (ASCP)