Many vendors are pushing different blades...but the only blade that is superior
is the Accu-edge. If you are using any other you will very likely get inferior
sections. That is 30 years of experience using Accu-edge blades always trying
others and always going back to the best.
-Original
Hey everyone.
I Need some help with my processor. It is a thermo scientific stp 120. I
came in today and when I went to puch the button to shut the alarm off, I
was shocked. The machine shocked me and then the little green display
screen went blank. I managed to get my blocks off the machine and I
They were using AccuEdge here for years and we changed to the Thermo MX35
Premier 3 years ago with no issues. In fact everyone was very upset for about
a month and then they did not want to change back. So, it really depends on
the knife and as I was told after using them for month they could
Thanks everyone for the suggestions..it's appearing that it was a bad lot of
blades. We switched to a new lot and there has been a noticeable improvement.
We are giving it a day or two to be sure it wasn't a combination of problems
but hopefully we will get beautiful sections once again!
Please No Recruiter Calls :
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State
Gastroenterology Associates is a multi-physician practice located in Northern
Kentucky. Its mission is To provide compassionate, high quality,
cost-effective care to patients' with
Hi, I'm trying to find out if anyone can tell me what can cause a sudden
distance change in the length of rotation on an HM 450 sliding freezing
microtome. range of motion was almost full length of microtome then suddenly
range is reduced to barely clearing base which causes a potential
Hi Histonetters!
I hope you are having a great day. I wanted to drop you a line and tell you
about some of the opportunities that I am working on and offer you a gift.
The gift is I have a copy of the Histology Professionals Day logo that you
can add to your signature or your Facebook page or
You should ask the manufacturer
René J.
From: Histo Joe histo...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2013 9:10 AM
Subject: [Histonet] Arm cutting length
Hi, I'm trying to find out if anyone can tell me what can cause a sudden
distance change in
Hello All,
One of our Neuropath docs is inquiring about a special stain for unencased
ameobas in cornea biopsies. I did a search and Gridley's Method was the
best option that appreaded. Is there someone that could either verify this
stain will work on this organism or let me know what you stain
Hi everyone,
We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin
following Oil Red O staining. We cannot get hematoxylin staining to work. We
are mainly seeing blue background but not labeling of nuclei.
Tissue is fixed with 4% PFA prior to sectioning. After tissue is
I own an old Zeiss microscope (Universal) and would like to have the
capability of performing polarized light microscopy studies.
I am particularly interested in exploiting the birefringent properties of
myelinated fibers in peripheral nerves.
My questions are:
1) what are the microscope parts
The standard amoeba stain is Heidenhain's iron hematoxylin.
René J.
From: Joseph Brooks jmbrooksokst...@gmail.com
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2013 11:12 AM
Subject: [Histonet] Unencased Ameoba Stain
Hello All,
One of our Neuropath docs is inquiring about
Mayer's hemaotoxylin (a progressive hematoxylin) is not adequate for FS.
Try Harris hematoxylin BUT stain first with the hematoxylin and with ORO after
wards to obtain better results.
René J.
From: Stacey Barrick barricksta...@yahoo.com
To: Histonet@lists.utsouthwestern.edu
The best and less expensive solution is to go to a photography store and buy a
plastic polarizing filter.
Cut a round piece and place it in the condenser filter ring and cut another (of
much smaller diameter) plastic filter and place it in the filter holder of your
microscope ocular.
The light
Matt Brooks asks: One of our Neuropath docs is inquiring about a
special stain for unencased
[amoebae] in cornea biopsies. I did a search and Gridley's method was
the best option that appreaded [sic]. Is there someone that could
either verify this stain will work on this organism or let me know
Hello everyone
I have been asked to collect the opinions of any users of the INFORM HER2
dual ISH for identification of breast cancer patients for Trastuzumab. I would
appreciate any feedback from users on technical issues, clinical value, or
other things you might wish to share. I appreciate
Stacey,
You wrote: You wrote:
We are trying to stain frozen cut sections of aorta with Mayer's Hematoxylin
following Oil Red O staining. We cannot get hematoxylin staining to work. We
are mainly seeing blue background but not labeling of nuclei.
Tissue is fixed with 4% PFA prior to
Hi all,
First time using histonet- I read earlier that reagents/chemicals used in the
lab with no expiration date are to be labeled with an expiration date of 5
years after having been opened. Is there literature on that? I need to update
my policy and prodedure for CAP. Also, this maysaound
Kathy, I actually posed that question to Fisher Scientific and got a letter
back saying that if the chemical is known to be unstable then they will give it
an expiration date (shown below). Chemicals that are very stable are considered
to have indefinite shelf life.
Here we label liquids at 3
Oh, BTW, we do label our distilled water with prep and expiration date when it
is put in bottles for use on the bench. It is not sterile and will grow stuff
if left too long. Usually we use it up quickly but it is good practice to know
when any reagent is made and expires. We give it a 4 week
Hello Everyone-
In case you missed it, our very own Jack Ratliff and Dr Heiko Richter's (not
our very own) Part 2 of their HistoTALK http://www.histotalk.com/ interview was
produced last week. Very interesting topic - Laser Histology! You're invited to
give it a listen.
Yours,
David
What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid?
Diana
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Until the staining starts to weaken
René J.
From: Diana McCaig dmcc...@ckha.on.ca
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 27, 2013 2:55 PM
Subject: [Histonet] Alcian Blue
What is the shelf life of prepared 1% Alcian blue in 3% Acetic
Why would you use a reagent until it starts to weaken, meaning the staining
intensity and results are changing over time. In my opinion that's really poor
quality control. Stain intensity, specificity and sensitivity should remain
constant.
Liz
-Original Message-
From:
A quick question: Does anyone bill an 88311 decal for processes done on the
bench?
Many thanks--!!
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT Tech at a time.
281.852.9457 Office
800.756.3309 Phone Fax
ad...@fullstaff.org
Sign up for the FREE
Alcian blue lasts until loss of dye onto the sections weakens staining. If one
runs many slides through it, it may not last 6 weeks. With light use, it may
last 6 years.
Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine
-Original Message-
From:
IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in
Dallas, Texas is seeking an Immunohistochemistry Technician
for its' Immunohistochemistry Lab. Responsibilities include slide
preparation (paraffin and frozen sections), IHC staining
Hi Stacey
I have used Mayer's Haematoxylin with an Oil Red O for more than 30
years and it works fine, You need to stain with the Hx before the Oil
Red O. 3 Minutes should be enough.
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology
Hi Matt
The Heidenhain's stain is good but if you want one that is prettier try
Gomori's one step trichrome. I have only ever used it for intestinal
protozoa but I think it would work for all.
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology
You're referring I suppose to the Gomori-Wheatley modification of
the one step trichrome, used for staining intestinal amoebae. Don't
know if it will work on tissue sections. It has quite a number of
variants.
Once again, I'd advise consulting an eye pathologist.
Bob Richmond
Samurai Pathologist
It is true that I have never used it on tissue sections as I have
usually performed it on smears for the Microbiology department.
However some of the specimens stained were formalin fixed and stained
well while others were PVA fixed.
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief
It's better not to assume that a stain developed for smears will work
on tissue sections without modification. The Gram stain is an obvious
case in point.
Bob Richmond
Samurai Pathologist
Maryville TN
*
On Wed, Feb 27, 2013 at 9:20 PM, Tony Reilly
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