Alcian blue lasts until loss of dye onto the sections weakens staining. If one runs many slides through it, it may not last 6 weeks. With light use, it may last 6 years. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, February 27, 2013 3:03 PM To: Rene J Buesa; Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Alcian Blue Why would you use a reagent until it starts to weaken, meaning the staining intensity and results are changing over time. In my opinion that's really poor quality control. Stain intensity, specificity and sensitivity should remain constant. Liz -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 27, 2013 11:57 AM To: Diana McCaig; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Alcian Blue Until the staining starts to weaken René J. From: Diana McCaig <dmcc...@ckha.on.ca> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, February 27, 2013 2:55 PM Subject: [Histonet] Alcian Blue What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
