Why would you use a reagent until it starts to weaken, meaning the staining 
intensity and results are changing over time.  In my opinion that's really poor 
quality control.  Stain intensity, specificity and sensitivity should remain 
constant.

Liz

-----Original Message-----
From: [email protected] 
[mailto:[email protected]] On Behalf Of Rene J Buesa
Sent: Wednesday, February 27, 2013 11:57 AM
To: Diana McCaig; '[email protected]'
Subject: Re: [Histonet] Alcian Blue

Until the staining starts to weaken
René J.

From: Diana McCaig <[email protected]>
To: "'[email protected]'" <[email protected]>
Sent: Wednesday, February 27, 2013 2:55 PM
Subject: [Histonet] Alcian Blue

What is the shelf life of  prepared  1% Alcian blue in 3% Acetic Acid?

Diana
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