Bruce,
We are very familiar with this problem. Yes, we have encountered slides that
are hydrophobic, and the reagents don't mix well on the slide. I have photos
of this happening in progress! We think it came down to humidity, it always
seems to happen in the summer months. We use Superfros
I've used Ventana for years and have never had that problem. We use Superfrost
Plus
slides from Cardinal. I can certainly see that their resolution would be too
time consuming and I too would be concerned that the staining would be false if
any reagent in the detection kit did not spread properl
Bruce,
Are you using the slides with the patient and control all in one. If so, is the
paint on the top of the slide or the reverse underside? There has been a known
problem for years that correlates to what you are describing with the control
box slides with paint on the top.
Secondly, have y
I've seen that when using the Dako stainer or manual staining. The small amount
of liquid used for the antibody and detection pools up on the slide rather than
spreading out. We solved it for those uses with longer soaks in TBS-tween
before putting the slides on the stainer and ensuring the rins
Histonians!
Has anyone else had incorrect IHC staining due to overly
charged slides that are hydrophobic? My Ventana service department was out
because I kept complaining about incomplete staining. For instance, the control
at the top of the slide stained with both DAB and hem
I have an ancient copy of Humason and she had methods 2 to 5 minutes for PAS
etc with Periodic Acid so I tried 2 min today. Will try more time and stronger
solutions and will also get around to doing some staining with PAS that I
wanted to do to look at goblet cells and compare them to the vacu
Hi Kathleen,
Yes,95% ethanol is fine.
It's (Methyl Benzoate) most likely misspelled in your protocol.
Try Sigma catalog # M29903.
-Damien L
--
Damien Laudier
Laudier Histology
917-836-7573
www.LaudierHistology.com
* *
___
Histonet mailing list
Hist
I was given a protocol for processing Drosophila brains into paraffin
manually, and it involves methyl benzoate. It is as follows:
Fixation: 3.5-4 hours in Carnoy's fixative, in hood (room temperature)
Processing: 2x30 min 99% ethanol (Would 95% be okay?)
60 min in absolute ethanol
Overnight i
I forgot to mention the timing. You may try 5 minutes or even 10 minutes
before staining in hematoxylin. It will give you even better results. I used 5
minutes in 1 % periodic acid.
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com
So, I'm late to this response, but there are several ways you can avoid
floaters. We utilize the ThinPrep process for all body fluids, bronch washes,
urines or other fluids we receive. The nature of this type of prep is
considered a method for reducing the potential for floaters. You could al
Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a
0.5% soln of that followed by a tap water wash and then dw then Harris
hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical
swine fever slides I looked at today. Very nice. I will play with th
11 matches
Mail list logo
