Hi,
Would anyone suggest me what staining is
best to color differentiate between cartilage and bone and epithelial tissues
in avian embryos?
I have been trying Mallory Trichrome for
embryos but recently I was suggested that Mallory Trichrome stains cartilage
differently
in embryos compared
Hi All,
I'm looking for a second hand fluorescent microscope.
Can anyone help? 220-240V.
E: mitch...@wanpost.com
Regards
Mitchell Wan
0418 745 750
P.O. BOX 2200,
Runcorn,
Brisbane,
QLD 4113
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It still seems to vary by market ( many factors including licensure in some
states), and by organization based on my observations. But I believe that there
is certainly a trend toward certification. At my organization they require
certification for any consideration, and also education to meet
While I understand the need for certification and continuing education, because
of regulations it is very hard to even have your OJT's sit for their
certification. Recently I interviewed candidates for an open position at my
facility. One candidate in particular looked very good on paper. I
This will be difficult discussion for many of us who were OJT in the 60s to
even into the 90s however; ASCP and states have now developed licensing
regulations that require through CLIA that almost prohibit the this track for
registration. The problem here is we have too few schools and
I've noticed that a few jobs at UPMC for histotechs require experience, but
not certification. You do have to be certified within a year of employment
though. I'm thinking this is due to the lack of research jobs, so people
like me (lab techs from research) are moving into hospital labs with
It's interesting to see the diference in aquisition of a person for
histolab. Here in Austria only biomedical scientist have the legal right for
working with clinical samples. Therefore one part of education deals also
with histotechnology and they have a few practical hours on microtome.
But I
Does anyone use CK5/6 + P63 + P504S orCK903 + P63 + P504S IVD? If
so can you share vendor and possibly procedure?
Lisa White HT(ASCP)
Supervisory HT
James H. Quillen VAMC
Corner of Veterans Way and Lamont
VAMC Warehouse BLDG. 205
PO Box 4000
PLMS 113
Mountain Home, TN 37684
Good morning everyone,
?
TGIF.? Just wondering if anyone out there is using ProExC on Thin Prep or
Surepath?? Also, what other markers are labs using with Thin Prep and SurePath?
?
Thanks in advance,
Gene
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I also test any bench HT's practical ability whether they appear with
credentials of certification, degrees or otherwise. They MUST have the
practical technical abilities to do well on the bench, enough said.
In an interview situation, what you have are some tools and bits of information
in
Hi all,
Does anyone have old Cell Vision journals from 1997? I am pretty sure it is
defunct and I have been trying to get a copy of a paper, can't find what I
need on the internet. I can provide the specific reference.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging
In case anyone was wondering, you do need to rebalance it. However, that
actually means level it, which is what I was going to do anyway!
I was thinking some inside part needed rebalancing, but this is not the
case.
Thanks for those who wrote in.
Emily
By bitching and bitching and bitching,
Well, my opinion is based on my own experience. I was hired as a histotech
with no prior histology experience besides what I completed in the
laboratory in college (This was only four years ago in Columbia, MO). I
trained on the job, then moved to be a histotech in Cedar Rapids Iowa. I
then
I recently had some tissues sent to me for frozen sectioning and staining. I
was able to section them just fine and also stained them reasonably well the
next day. The stains that I used were ones that I have used many times on
similar sections. The stains did not seem as crisp as usual but the
It can be difficult to find employment without certification, however I have
seen techs with certification who cannot perform. It does depend on the
facility and the environment. Some say it is for liability purposes (in case
there is a case that was mishandled) and some say for the
The interesting thing is the history of Histotechnican and Histotechnologist.
We have probably all been called both if we have been in this field very long.
Recently, I had a reason to find out how it actually came about and where the
two titles came from originally and got so confused.
Very well explained and summarized Pam, thank you.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: pamar...@uams.edu
To: tnma...@mdanderson.org; histonet@lists.utsouthwestern.edu
Date: Fri, 23 Aug 2013 19:16:44 +
CC:
Subject: [Histonet] RE: Uncertified histotechs
The interesting
Your explanation of how we became Histologist Soup is very enlightening while
being somewhat frightening. I have experienced this category confusion myself.
I am an HT who did the OJT route and then took the written/practical exam in
1968. I chose not to do the HTL because I was (and was
Thank you everyone for your interest in this subject. I get an overwhelming
feeling that being certified is practically necessary to apply for histotech
jobs. It seems like if you lack certification then you will be lucky to find a
place interested in you. Even if you have all the experience.
Once I applied for jobs I got hired relatively quick and I could barely make
a decent slide.
Don't feel bad about that. That is what entry level is all about. It reminds me
of my first week as an electron microscopy technologist (which is what I did
before learning histotechnology). I went to
I know a gentleman who is HT(ASCP) certified. The facility where he used to
work gave him a certificate of appreciation and on it he was referred to as a
Histotechnologist. I asked when he got his HTL and he stated where he worked
they call you that after a period. That really ticked me off.
I'm trying to preserve whole sheep testes, and I'm looking for some advice on
fixing time. I'm using 10% neutral buffered formalin, and they've been
submerged for about a week. Should I continue fixing, or transfer to ethanol?
I have no idea how long it needs.
I'm trying to do long term
At one time I did a lot of work on cartilage and growth plate
and used Toluidine Blue and Fast Green. T-blue (buffered appropriately)
stains the
proteoglycan a lovely metachromatic blue and the bone and most
everything else
is green. The nuclei of cells are also green since we used no nuclear
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