Hi,
Please clarify why this answer to the HistoDeck study question is a) and not
b).
Here is the question:
'Frozen section slides cut from fresh, unfixed tissue specimens are optimally
fixed in which of the following solutions?
a) 37%-40% formaldehyde
b) Cold acetone
c)
I believe you may need to have the unit serviced. It sounds like something is
not tight enough, perhaps the stage or blade holder unit. You said you secured
everything which makes me think you have some issue with the cryostat itself.
If a sharp blade, tightened blade and specimen, and
Personally, I think it's a is a wrong answer, and that you are correct
that b is a better answer. My students and I have found a couple of other
questions that we thought had the wrong answer indicated in the study set.
Peggy A. Wenk, HTL(ASCP)SLS
-Original Message-
From: Stephenson,
I just attended Jan Minshew's workshop on cryostats at the NSH Symposium in
Providence, RI, and she brought up something I had never thought of that
causes thick and thin.
If the handle that tightens the blade in the blade holder is over-tightened,
the blade will become bowed, and that will
For frozen cut sections, would the fixation also depend on what you plan on
doing with it. For example, HE, Special Stain or IHC? Please correct if I am
wrong. I think that is a trick question!!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-
We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
Hi Histonetters!!!
How are you doing?
The NSH was AMAZING!
Providence was GORGEOUS
The Speakers were BRILLIANT
CONGRATULATIONS TO THE 2013 Leadership Education And Advocacy Award Winners:
Histotechnologist of the Year-Wanda Jones
J.B. McCormick Award- Peggy Wenk
President's Award-
Thank you All!
Sheryl Stephenson | Histology Technician
-Original Message-
From: Bernice Frederick [mailto:b-freder...@northwestern.edu]
Sent: Thursday, October 03, 2013 9:43 AM
To: Watson, Linda; Lee Peggy Wenk; Stephenson, Sheryl;
histonet@lists.utsouthwestern.edu
Subject: RE:
I agree, there is probably more than one correct answer to this question,
depending upon whether you are planning on doing stains for lipids, IHC,
immunofluoresence or muscle enzymes.
But I don't think (A) full strength 37-40% formaldehyde solution would ever
be the correct answer. Unless you
When tissue processing was manual there were some gadgets providing vacuum
and those using it reported better results. The fact of the matter was that
manual processing is so slow that anything you introduce will favor the process.
Static tissue processors, i.e. those that only mover the
Honestly I think this all depends on what they are actually asking. Are they
asking what you fix the tissue in after the frozen is complete and you need to
submit the remaining tissue for routine processing? If so, then A is the
correct answer.
Are they asking what you fix the tissue to the
Dear Histonians
I'm in the market (please, no vendors) for a new microtome. I'd
like an automated/manual type. What is on the market these days that is worthy?
I know about Leica, but it's been many years since I've looked for these
instruments and would love to know what you
I'd go with A, but it really depends on what you are going to do with the
sections after fixation.
In the protocol for Oil Red O in Freida's second edition (that I use almost
daily combined with some steps from PolyScientific's ORO protocol), step #1
says to fix in 40% formaldehyde. Doesn't
Still Leica
René J.
From: Bruce Gapinski bgapin...@pathgroup.com
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Thursday, October 3, 2013 10:46 AM
Subject: [Histonet] Microtomes
Dear Histonians
I'm in the market
Still don't have use for fully automated. 1st microm , 2nd Leica
Opinion only
Sent from my Verizon Wireless 4G LTE Smartphone
- Reply message -
From: Bruce Gapinski bgapin...@pathgroup.com
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Subject: [Histonet]
Leica.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski
I would go with Leica. I am getting them for my lab.
Haley H.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea
DeBrosse-Serra
Sent: Thursday, October 03, 2013 9:23 AM
To: 'Bruce Gapinski';
We also use this oil red O method and use the 40% formaldehyde.
The questions lacks enough information to correctly answer it. I am sure
the author of the question had something in mind and other options didn't
occur to him/her at the time.
Jennifer
From: Grantham, Andrea L - (algranth)
Thermo Shandon Finesse is my favorite. Manual version requires oiling which
is easy and gives you a good reason to keep it clean inside. I got one for
about 6,000. The high end automatic version is the Finesse ME+ and is the
best microtome I have ever seen. Someone that has never cut before could
Leica always
Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4140 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
From: histonet-boun...@lists.utsouthwestern.edu
For those of you who use the 40% formaldehyde, how long is you fixation time on
frozen slides? We use 10% NBF with 1 minute to fix, but sometimes it gets
hectic if you have multiple frozens all at once.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Does anyone in Histoland know of a reference lab that offers this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
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Histonet mailing list
It would dissolve the fat if you used acetone wouldnt it? Without knowing the
tissue type or stain, the answer is A. All other choices dissolve fat I think.
Good luck on your exam!
Sent from my iPhone
On Oct 3, 2013, at 8:15 AM, Lee Peggy Wenk lpw...@sbcglobal.net wrote:
Personally, I
We use 36-40% formaldehyde for a minimal time of 2-3 dips for fast frozen
HE. On the other side the slides stand in the solution as long as all
frozens are already cut.
Commercial formaldehyde contents a good part of methanol (a MSDS says
5-15%). So fast fixation is a combination of formaldehyde
Hello -
Our research group does a fair amount of autoradiography with frozen sections
and we sometimes perform IHC or routine stains. I am not a histologist (nor do
we have one in our current group), so I assumed that the correct answer was
alcoholic formalin, because the other options were
Surely I will find those that disagree with this post, however what I was
classically trained about fixation categories generally falls within the
information below...which I took the liberty of reposting here since it is
pretty clear straightforward from Leica.
http://www.ihcworld.com/_protocols/general_ICC/fixation.htm
This article specifically addresses the differences. You are right about the
cross linking, but alcohol and acetone are still considered fixatives.
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
Hi
Thanks to every one that sent me Acid Phosphotase procedure for muscle
biopsies. I also need some help with the bodian stain, we are not getting any
staining on the slides, we have a procedure that they used years ago and now we
can't get it working (we did purchase new chemicals) and we
Hello histoneters!
This is my first time using the net. I hope to find here some answers to my
problem. I am working with free floating sections embedded in gelatin. I need
to use Proteinase K to detect alpha- synuclein aggregates in brain tissue. I
tried concentrations in the range of 1 Ug/ml
the protocol calls for 1 minute in the 40% formaldehyde and then rinse the
sections well.
From: Rathborne, Toni trathbo...@somerset-healthcare.com
To: 'Jennifer MacDonald' jmacdon...@mtsac.edu, Grantham, Andrea L
- (algranth) algra...@email.arizona.edu
Cc: HISTONET
We perform Gram Twort for our gram stains. It is essentially the same as the
gram stain used in microbiology except that we counter stain with a neutral red
fast green which is very effective.
Cheers Cate
Cate Hardy
Senior Technical Officer
Veterinary Diagnostic Laboratory
School of Animal
HistoDeck questions are companion of the Carson' s book. Carson fixes in
37-40% formaldehyde for OilRed O.
Any acetone or alcohol will dissolve the fat and OilRedO will be negative
or suboptimum.
The answer should be A
Mesru
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Histonet mailing list
Hi Everyone, this is my first time posting, hope this works. One of our
pathologists is interested in the Dacie iron stain for bone marrow specimens.
Where can I purchase this stain or is this simply a method? I greatly
appreciate your help!
Melissa
Anatomic Pathology Supervisor
Nemours
Hi Helene
Have you tried a Bielschowsky instead. It is widely reported to provide better
staining than the Bodian.
http://www.ncbi.nlm.nih.gov/pubmed/2422580
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Hi there
We recently purchased a Leica microtome and all seems to be going very well. It
cuts beautifully. Just warn students not to clean the machine with xylene as
ours did and smudged the writing on the microtome.
Good luck
Subash Govender
Anatomical Pathology Research Lab
University of Cape
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