[Histonet] test

test mail - best wishes to all Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Troponin is biomarker of heart attacks

Hi Jorge - If I understand your question correctly - it is because cardiac troponin is specific for cardiac muscle damage. Other muscle enzymes/proteins we can assay for could also be elevated with skeletal muscle damage. -Nancy Stedman From: histo

Re: [Histonet] Troponin is biomarker of heart attacks

troponin I is the only troponin assay to show cardiac muscle injuty. -Original Message- From: Jorge A. Santiago-Blay To: Histonet Sent: Thu, Feb 5, 2015 3:02 pm Subject: [Histonet] Troponin is biomarker of heart attacks Dear Histonnetters: I am not sure the range of expertise of the

[Histonet] Re: ALCIAN YELLOW ALCIAN BLUE 8GX

Dear Sirs, We are manufacturer of ALCIAN BLUE 8GX and ALCIAN YELLOW. Looking forward to hearing from you asap. Kind Regards Minggeng Wang, Ph.D/President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road, Suzhou New & Hi-Tech District, 215011 China Tel: 0086 512 68246939 Fax: 0086 512 68258994

Re: [Histonet] denatured alcohol

You will have to start in 80% "ethanol" and you cannot assume the tissue is dehydrated enough.René J.  On Thursday, February 5, 2015 3:57 PM, Jorge A. Santiago-Blay wrote: Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the col

[Histonet] Troponin is biomarker of heart attacks

Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjo...@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explan

[Histonet] denatured alcohol

Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the colleague to let me know what was the concentration of ethanol in the sample and the answer was "about 80%". From the smell, I suspect the rest is methanol. I need to dehydrate the sample th

[Histonet] TRAP staining FFPE sections

Hi all, I just performed TRAP staining on ffpe edta delcaled bone sections using the Sigma kit (#387A), and was wondering if anyone had experience with reducing the yellow staining over the entire tissue due to the Fast Garnet GBC. I had positive TRAP staining that is red, but there is a lack of c

[Histonet] Job opportunity: Research Histology Technician in Bastrop, TX

Job opportunity for a research histology technician at MD Anderson Cancer Center at the BASTROP, TX location. (approximately 35 miles from Austin, TX) http://jobs.mdanderson.org/texas/research/jobid6803661-research-histology-technician-bastrop-texas-jobs Beth K. Chaffee, DVM, PhD, DACVP Assis

[Histonet] Rick

Rick had a situation at home and had to leave about noon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Recall: Rick

Scott, Catherine L would like to recall the message, "Rick". ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Cryosectioning undecalcified bone

Hello, I believe that the products for the CryoJane tape transfer are still available from Fisher. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St  Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231 410-614-1660 http://tmalab.jhmi.edu

RE: [Histonet] Cryosectioning undecalcified bone

Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-39

[Histonet] RE: Leica ASP basket transport

Not sure what your baskets look like, but we found "Rubbermaid-like" travel shoe boxes at Walmart that work really well for us. They're deeper than what we found in the kitchen department. Look by the luggage and travel stuff. (We also haunt the plasticware sections of stores... :D) Laura

[Histonet] Cryosectioning undecalcified bone

Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow

RE: [Histonet] paraffin sectioning-dry tissue? - long response - image analysis related

Lucie You are not overthinking this at all if you are utilizing your sections for any image analysis applications. You need to be able to standardize as much of the histology process as possible. There are so many other parameters that can cause section thickness to fluctuate. Such as the sh

Re: [Histonet] paraffin sectioning-dry tissue?

By adding water, ice, or warm humidity (through exhalations) to the mix, though, doesn't the block contract/expand? Wouldn't that change the ultimate thickness of the section? I've always wondered how much it affects things. Could your sections be 1 um (or multiple um!) thicker/thinner that you ex

[Histonet] RE: Leica ASP basket transport

Glad to know I'm not the only one who haunts the plasticware sections of stores looking for the ideal container for the lab! We use some Rubbermaid containers for the Peloris and VIP baskets, but they are not fully 3" high. I suggest looking at Bed Bath and Beyond, Container Store, all supermar

RE: [Histonet] Leica ASP basket transport

Good luck probably some type of plastic ware that u can find at wallmart Sent from my Verizon Wireless 4G LTE smartphone Original message From: "Ingrao, Nicholas" Date:02/05/2015 11:19 AM (GMT-05:00) To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] Leica AS

[Histonet] Leica ASP basket transport

Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Bask

RE: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer

We have one too. We have been getting ribbons from Surgipath/Leica. The number is 38V0590001F. we have Tech One Biomedical service it. They have been great. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwes

RE: [Histonet] paraffin sectioning-dry tissue?

We use a 5% glycerin in denatured alcohol for our 100% alcohol on our tissue processor for routine animal tissues, this reduces the over dehydration of the animal tissue and greatly reduces the time required to soak the blocks. Warning, if processing fat or cell pellets do not use this, we swit

[Histonet] Looking for ribbons for old Surgipath VCP cassette printer

Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman _

[Histonet] C-Kit

Hi All, I have a pathologist that is wanting me to bring in C-kit. My question is am I required by CAP to also do the Survey for predicative markers for C-kit? Thanks for your time and answers. Mike ___ Histonet mailing list Histonet@lists.utsouthweste

Re: [Histonet] paraffin sectioning-dry tissue?

Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sec

Re: [Histonet] paraffin sectioning-dry tissue?

This is common with mouse and rat tissues, they get "over-dried" with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: Hello all

[Histonet] use of denatured alcohol

Hello, Our lab is considering switching to denatured alcohol as a cost saving initiative. Is anyone using denatured alcohols and performing subsequent Class II IHC testing with no impact to results? Thank-you, Joanna Joanna Bartczak MLT II - Immunohistochemistry Calgary Laboratory Services 40

Re: [Histonet] paraffin sectioning-dry tissue?

After macro trimming into our blocks (very gently if friable or dry) we place them on an ice tray to soak for a good while. Each well is filled with a little bit of water - this rehydrates the tissue just enough to get a few good sections off the top. we¹ve had success with this method for most any

[Histonet] paraffin sectioning-dry tissue?

Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing

[Histonet] Substitute for Safran du Gatinais

I am doing the Movats Pentachrome stain which requires the use of Safran du Gatinais. I would like to know if there is any substitute for Safran du Gatinais. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailm