Re: [Histonet] Melanin Bleach

The KMnO4/oxalic acid sequence will weaken the tissue adherence. If you do not have to use Peroxidase/DAB, then I would recommend alkaline phosphatase labelling (+red chromagen). Remember KMnO4/oxalic acid can be deleterious to some antigen-antibody reactions. We found that LCA, CAM5.2, L26,

Re: [Histonet] Cytology/Histology Staining Question

Yep, We do. We have a separate dehydration sequence for PAP and other special stains (separate from the eosin dehydration sequence). We have a Leica (used to be Vision Biosystems) Autostainer Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the

[Histonet] Cryojane Oct artifact

I am getting a bubbly artifact on my tissue due to the OCT not being washed off the slide after putting it on a cryojane slide and flashing it and staining with H Is there a trick to dissolve the OCT away Sent from my iPhone ___ Histonet mailing list

Re: [Histonet] Melanin Bleach

Cool trick Jeffrey! On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Karen, > The bleaching reagents will not compatible before IHC anyway. > Some tips: > 1. Do IHC-test as usual and counterstain nuclei with methylene blue > Loeffler at

[Histonet] P24

Does anyone know of a supplier for P24 control slides? Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] p16 Nordi QC

There is a fantastic resource for antibody assessment and optimization and they have done p16 as well as many many other antibodies. Their results of testing is free on line. p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009 with many different companies antibodies. Here is the

Re: [Histonet] Melanin Bleach

Karen, The bleaching reagents will not compatible before IHC anyway. Some tips: 1. Do IHC-test as usual and counterstain nuclei with methylene blue Loeffler at 3-5 sec, only for profile of chromatine. The melanin will stain at green color. 2. Use any red color chromogen for IHC. It will be some

[Histonet] Open Position

Hi- Job Opening: OSU Histology Lab, LLC Columbus, Ohio Assistant Laboratory Manager for a small reference based laboratory. Simple grossing and histology are performed. Musts: Graduate of a school of histology with a HT/HTL certification or equivalent. A minimum of an associate's degree in a

Re: [Histonet] Cytology/Histology Staining Question

Mary, We run both protocols on the same stainer, however, each protocol has it own set of reagents except for the common water wells and the two xylenes at the end before the final xylene. We have the Leica ST 5020, which has 36 wells not including the 2 oven wells. Hope this helps.

Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

This is the CAP regulation that talks about cross contamination. CYP.04150 Cross-Contamination Phase I There is a written procedure to prevent cross-contamination of specimens during processing and staining. NOTE: Procedures must prevent cross-contamination between gynecologic and

Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

Hey Mary, The problem is not the machine, it is the reagents sharing that is the issue. You can use different reagents and protocols for the Histo and Cyto slides and on the same instrument. You might even get away with changing your Alcohols and filtering everything else and see how that

Re: [Histonet] Melanin Bleach

You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in

Re: [Histonet] Melanin Bleach

Hi Karen- I have also had problems getting tissue being stained for IHC markers to adhere to the slide. The Potassium permanganate is really harse on the tissue (if you can get the sections to stay on). Here is a trick I picked up at an NSH lecture that I have used successfully several

[Histonet] Melanin Bleach

Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had

[Histonet] ASCP Certified Pathologist Assistant Job Opening-

Hello, I have a Full Time/Permanent job opening for an ASCP certified Pathologist Assistant in Ohio. Please contact me for details. Have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572

Re: [Histonet] Cytology/Histology Staining Question

I would be concerned with potential cross-contamination. In my lab we had 2 staining instruments, one for cytology and other for histology.René On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" wrote: Hello all, I work in a small, low

[Histonet] P16

Has anyone found a way to do p16 staining without purchasing anything from Ventana? My company wants to do P16 but refuses to by any ventana products and I have explained it is a waste of money to keep testing the antibodies from all the other companies. -- Charles Riley HT(ASCP)CM