Re: [Histonet] No Ribbon

I might get some individuals upset but we really need to watch what we do in the histology lab and how that effects the precision of our process. When you breath on the block you are basically generating a thicker section, it's not a good practice to keep. Trust me I used to do this in the

Re: [Histonet] No Ribbon

Point taken, thanks Elizabeth. My clinical days are long gone, but that is an important consideration. yours, mills On Tue, Apr 11, 2017 at 11:13 AM, Elizabeth Chlipala wrote: > I might get some individuals upset but we really need to watch what we do > in the histology

Re: [Histonet] No Ribbon

Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together :) mills On

Re: [Histonet] No Ribbon

Hi, the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon. Try gently cleaning with xylene, followed by alcohol before sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX

Re: [Histonet] No Ribbon

Yes, I use ice for my blocks and get them as cold as possible. It is a high profile blade holder. The paraffin is good for embedding and processing. I was told to use a low angle for Statcut blades, but I will try it at 10. Thanks -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma

[Histonet] NY Histotech

Hi Histonetters! Do you know of anyone who has a NYS laboratory license? I have a fantastic private lab client on Long Island (1hr outside of Manhattan). They are looking for someone to fill their Full-Time, permanent, Day-Shift Histotech role! It is a great environment in a great location, and

[Histonet] No Ribbon

I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at

[Histonet] Anti-fibrin antibody

Dear all, I've been trying to optimise an immunohistochemistry protocol for fibrin that has not been working. Was wondering if there is anyone that would have any recommendation of staining protocols for fibrin or recommendation of anti-fibrin antibodies? We are working with cryosections,