[Histonet] About Amoebae infected Tissue for Undergraduate student Practical class

2018-02-14 Thread Nilufa Sultana via Histonet
Dear Histo-netter, It will be appreciated if I can get information for the following- 1. Any source to get Amoebae infected Animal/humane tissue/Block to cut about 800 slides for the undergraduate Anatomy Pathology Practical class for staining with PAS 2. Any method to make

Re: [Histonet] Deplastifying MMA

2018-02-14 Thread Dr. Michael Gudo (Morphisto GmbH) via Histonet
Hi Dorothy, Hi Reuel, MMA embedding and deplastination is of course a very difficult process and many protocols are discussed, but only a few work well. So in general: real deplastination cannot be performed by xylene or acetone alone, these solutions are just a pretreatment. The best way for

Re: [Histonet] [EXTERNAL] Deplastifying MMA

2018-02-14 Thread Garcia, Lori, M.Sc. via Histonet
Hi Reuel, One thing you could try is THF (tetrahydrofuran). We have used it in the past to rapidly dissolve the MMA on slides. Good luck, Lori -Original Message- From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, February 14, 2018 11:46 AM To:

[Histonet] Deplastifying MMA

2018-02-14 Thread Dorothy Hu via Histonet
> > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustratingI know. Best luck. Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 + > From: Reuel Cornelia

Re: [Histonet] processing Dosophilla flies

2018-02-14 Thread Hobbs, Carl via Histonet
Hi Processing them , how? To Pwax? To snapfreeze? To resin? Kind regards Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge  Kings College London London SE1 1UL   020 7848 6813 ___ Histonet

[Histonet] (no subject)

2018-02-14 Thread seaduster--- via Histonet
Hey Histonet https://goo.gl/pmPPmg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet