Dear Histo-netter,
It will be appreciated if I can get information for the following-
1. Any source to get Amoebae infected Animal/humane tissue/Block to cut
about 800 slides for the undergraduate Anatomy Pathology Practical class for
staining with PAS
2. Any method to make
Hi Dorothy,
Hi Reuel,
MMA embedding and deplastination is of course a very difficult process and many
protocols are discussed, but only a few work well. So in general: real
deplastination cannot be performed by xylene or acetone alone, these solutions
are just a pretreatment. The best way for
Hi Reuel,
One thing you could try is THF (tetrahydrofuran). We have used it in the past
to rapidly dissolve the MMA on slides.
Good luck,
Lori
-Original Message-
From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 14, 2018 11:46 AM
To:
>
> Hi Reuel
>
We had the same thing as you. It was caused by bad MMA, I tested. Write
down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
was very frustratingI know.
Best luck.
Dorothy
> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia
Hi
Processing them , how?
To Pwax?
To snapfreeze?
To resin?
Kind regards
Carl
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL
020 7848 6813
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