[Histonet] FW: ER/PR question

2019-02-07 Thread Tony Henwood (SCHN) via Histonet
This paper might be useful: Ehinger, A., Bendahl, P. O., Rydén, L., Fernö, M., & Alkner, S. (2018). Stability of oestrogen and progesterone receptor antigenicity in formalin‐fixed paraffin‐embedded breast cancer tissue over time. Apmis, 126(9), 746-754. Regards Tony Henwood JP, MSc, BAppSc,

[Histonet] Cut-resistant gloves and SOP for frozen brain

2019-02-07 Thread Jones, Lynne via Histonet
Hello - I would appreciate feedback from the HistoNet community on best practices for handling frozen human (or macaque) brain for autoradiography in a research lab. I'm also curious about lightweight cut resistant gloves, and whether they are practical for anything more than changing blades (if

Re: [Histonet] Histonet Digest, Vol 183, Issue 6

2019-02-07 Thread John O’Brien via Histonet
To whom it concerns My company for 30 years have sold refurbished vip tissue processor The refurbishment was not done properly your ceramics Value must have small cracks in it allowing chemical to creep into other chemicals on process If u bought this vip from a reputable service company it

Re: [Histonet] ER/PR question

2019-02-07 Thread Bob Richmond via Histonet
Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco, CA asks: >>One of my pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay?<< You can only try. There

Re: [Histonet] ER/PR question

2019-02-07 Thread Allan Wang via Histonet
The antigen doesn't decay much inside the tissue block, as long as the sections were freshly done. We redo ER/PR on archived samples from 15 years ago and they still stain very strongly. Allan On Thu, Feb 7, 2019 at 8:26 AM Heckford, Karen - SMMC-SF via Histonet <

[Histonet] Fwd: VIP 5

2019-02-07 Thread Patti Nelson via Histonet
Patti Nelson HT (ASCP) 909-841-9761 Sent from my iPhone Begin forwarded message: > From: Patricia Nelson > Date: February 7, 2019 at 8:38:23 AM PST > To: "nelsonr...@verizon.net" > Subject:

[Histonet] bachelors of science

2019-02-07 Thread Piche, Jessica via Histonet
Hello Everyone, Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. Thanks in advance. Jessica Piche, HT(ASCP) Waterbury Hospital ___

Re: [Histonet] ER/PR question

2019-02-07 Thread Rene J Buesa via Histonet
Time alone is not the only cause for antigen decay for it is also associated with storage conditions and, as such, you cannot "predict" % decay based on time alone.Just try one block and let the pathologist decide based on the usefulness of what he sees in that try test.René On Thursday,

[Histonet] Histotech Job in New Hyde Park, NY Area Full Time/Permanent

2019-02-07 Thread Melissa Owens via Histonet
Good Morning, Quick note to spread the word about my Full Time Permanent Histotech job in New Hyde Park, NY area. Please contact me for job description/shift/details. Thank you, Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117

[Histonet] ER/PR question

2019-02-07 Thread Heckford, Karen - SMMC-SF via Histonet
Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450