Re: [Histonet] Coverslipping mystery

this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re

[Histonet] Coverslipping mystery

! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Frozen section artefact

Hi again, In your opinion is it better to fix frozen sections prior to storage in -80 or following removal from -80 prior to a run? Thanks Adam -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl

[Histonet] Frozen section artefact

stained PAS to highlight), nuclear degredation. Could it be fixation? We air dry following sectioning and fix in Acetone / alcohol 3:1 for 5 mins. Any ideas would be great! Thanks in advance. Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX This message

[Histonet] What is this item called?

microscope. It gives me much better working angles than the spot plates I resort to when I cannot find this item. If anyone can identify the item or, even better, tell me where I can order more, please let me know. Thank you very much. --Adam Adam Haberman Visiting Assistant Professor of Biology

Re: [Histonet] What is this item called?

Barry, It is 3 x 1 3/4, and the depression has a 1 1/2 diameter. Thanks for all the ideas so far. It isn't a hanging drop slide or a watch glass. You can see a picture of it next to a depression slide here: http://imgur.com/yBwvw I don't see a better way to send a picture on this list. --Adam

Re: [Histonet] What is this item called?

Jay, there are no markings on it at all. They are making it hard for me to give them money. --Adam Adam Haberman Visiting Assistant Professor of Biology Oberlin College adam.haber...@oberlin.edu (440)775-6502 On Tue, Jul 3, 2012 at 3:32 PM, Jay Lundgren jaylundg...@gmail.com wrote

[Histonet] Translucent patch

of the tissue causing this patch? Any ideas would be great, Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] Whiskers

to soften the keratin prior to embedding? I am thinking 10% Sodium Hydroxide or 5% Ammonium Hydroxide but do not really know about appropriate timings for such a small tissue. Many thanks, Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX

[Histonet] RE: KP Markers!

If you would like to obtain KP Markers, they are now being carried by Sensor Health, inc. in Cambridge, Ontario, Canada. If you have any questions you can contact Adam Harris at 888-777-7080 or 519-241-2194. You can check out their website at www.sensorhealth.com

[Histonet] Specimen Clamps Leica

Hi I'm looking for some used specimen clamps for a Leica RM 2255. I'm interested in any clamp that is for holding round or irregularly shaped specimens. Thanks Adam  ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] So long, and thanks for all the help

to the group. You are an amazing group of people. Sincerely, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] protocol for in situ RT_PCR

I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse bones with good results. It's possible that the alcohol is denaturing your antigens of interest. Is there a reason you need to use alcoholic formalin? Adam On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank fli...@wellstatoc.com

Re: [Histonet] Decalcification of bone

are you currently decalcifying? Are you paraffin embedding or cutting frozen sections? What is exactly happening when you're cutting the bones that gives you poor quality? What is your end goal (IHC, IF, HE)? Adam On Thu, Aug 11, 2011 at 3:10 AM, Rachael Glebocki rachael.glebo...@nottingham.ac.uk

[Histonet] Cutting frozen decalcified mouse bones

like overkill to me, but I need these sections ASAP. Ideas? I have a whole bunch more bones I need to cut over the next few weeks... Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

Re: [Histonet] Light microscopy to get orientation for fluorescent

Try differential interference microscopy (DIC). Many fluorescent scopes will have this as an option. It will give you faint outlines of the cells. Adam On Sun, Jul 17, 2011 at 1:01 AM, Otto Strauss olstra...@gmail.com wrote: I am trying to do fluorescent immunohistochemistry on liver tissue

Re: [Histonet] pan B-cell marker for frozen mouse tissue

, although pre-pro B cells do not express CD19. If you're looking at any organ other than bone marrow or spleen, there shouldn't be many pre-pro B cells and you can probably just use CD19. Adam On Mon, Jul 11, 2011 at 1:37 PM, Michele Wich mw...@7thwavelabs.com wrote: I am trying to find the most

[Histonet] Sad news

of the technical expertise she provided to the scientific community, she will definitely be missed. For those of you who knew her personally, I'm sure she will be missed much more. Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Flk2 / Flt3 / CD135 on mouse bone

way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Flk2 / Flt3 / CD135 on mouse bone

Hi Ray, That is a great idea. We are indeed looking for Flk2 expression on hematopoietic progenitors cells, but alas, we are looking at these cells in relationship to bone elements such as osteoblasts, so we need to do it on intact bone. Adam On Mon, Jun 27, 2011 at 8:43 PM, koelli

Re: [Histonet] antigen retreval method-mouse femour

retrieve with citrate buffer pH 6.0 for 10 mins at 95C. The bone generally stays intact, although the morphology is rarely as nice as unretrieved sections. Some people swear by other antigen retrieval methods that don't involve heat, but I've never managed to get them to work. Adam On Sun, May 29

[Histonet] Xylene re-use SOP

in enforcing all sorts of vague regulations. Any suggestions are welcome. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GFP labelled cells after decalcification...are they still labelled?

and decalcify using EDTA, and use frozen sections. I hope this helps, Adam On Thu, Mar 31, 2011 at 12:42 PM, James Hart jameshar...@gmail.com wrote: Hi All A first time user of Histonet here -with a quick question for research histologists... Has anyone ever attempted to locate GFP expressing cells

[Histonet] Mouse CD45 staining of bone marrow

. I was wondering if anyone has IHC or IF photos of CD45 staining in mouse bone marrow so I know what it should look like. Any suggestions are welcome. Thanks, Adam The specifics: 1) Fix the bones overnight in 4% PFA at 4C 2) Decalcify in EDTA for 3 days at 4C 3) Cryoprotect in 30% sucrose

[Histonet] tungsten carbide knives

Hi   If anyone has any used tungsten carbide knives that they no longer need please let me know.  These should be in good shape and suitable for sharpening. I'm happy to pay a resonable price for them.   Thanks   Adam --- On Thu, 2/10/11, histonet-requ...@lists.utsouthwestern.edu histonet

Re: [Histonet] Re: Banana smelling chemical in our formalin

That wasn't formalin. It was Histologie for Men, the new banana-scented cologne for histotechs. But seriously, I guess it's formalin ethyl acetate, which smells vaguely banana-like and is used for extraction of parasites from stool samples. Adam On Mon, Jan 24, 2011 at 4:56 PM, Patrick Laurie

Re: [Histonet] necropsy on freezed animal samples

tissues. So I would agree from experience that this is not a good idea. Adam On Fri, Jan 21, 2011 at 10:21 AM, Geoff McAuliffe mcaul...@umdnj.eduwrote: If the tissue is not fixed soon after death the microscopic morphology will be terrible, no matter whether the animal is refrigerated or frozen

[Histonet] Contract Histo Tech Needs in South Carolina

, no weekends, no holidays. Contract will go for 17 weeks in length with possibility of extension. Please contact Adam Schultz @ (813) 371-3427 or Kevin Lucania @ (813) 371-5174. They can both also be reached toll free at 888-800-1855. ***Note - Minimum two years experience required to apply

[Histonet] Toluidine blue staining of CFU-F

found was for staining of mast cells in sections, but that's dissolved in 70% ethanol and very acidified. I'm not sure that's what I want. Has anyone done this before? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Job opening for a Histology Manager at HealthTronics in Augusta, GA 30901

Hello, my name is Adam, I am a recruiter at Adecco Medical Science and I have an opening for a Histology Manager in Augusta, GA 30901. This position will fill quickly so if you are interested please reach out to me as soon as you can. I have listed the job description and required

[Histonet] I would like to post this mesage on to all subscribers on histonet

Job opening for a Histology Manager in Augusta, GA 30901 Hello, my name is Adam, I am a recruiter at Adecco Medical Science and I have an opening for a Histology Manager in Augusta, GA 30901. This position will fill quickly so if you are interested please reach out to me as soon as you can

Re: [Histonet] IHC theoretical question

hard. Most antigens don't nonspecifically bind stuff, so you're good. Is that clear? Adam On Fri, Dec 24, 2010 at 10:30 AM, wassan alkadhumi w_alkadh...@yahoo.comwrote: Dear histonet members I have a theoretical question concerning IHC, we do HRP method using DAKO materials, first step

[Histonet] Hazy background during IF

problem) Put on coverslip Dab corners of coverslip with clear nailpolish Let sit overnight for mounting media to cure Any ideas what could be going on? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] Question from one of our researchers

While not a cell membrane marker, you can often use differential interference contrast (DIC) microscopy. The microscope I use has a special setting that lets you add DIC without any additional staining. In the mouse bone marrow paraffin sections, it lets you see most edges of most cells. Adam

[Histonet] Paraffin liver sections

routinely cut decalcified bones on that microtome and have never seen that. Any ideas on what is going on? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Paraffin liver sections

water for several minutes. It fixed most of the problem, at least for now. You people are far too helpful. Thanks, Adam On Thu, Nov 4, 2010 at 2:53 PM, Jay Lundgren jaylundg...@gmail.com wrote: Sounds to me like they are overprocessed (dried out). Try soaking them on ice for 30 minutes or so

[Histonet] (no subject)

Hi,   Does anyone have a blade holder, tungesten blade and clamp for holding specimens that would fit on a Leica 2265 ? I'm trying to upgrade a microtome set up for paraffin sectioning to one that can handle resin embedded sections.   Many thanks   Adam

[Histonet] Microtome for undecalcified tissues

Hi,   I'm looking for a good used sledge or rotary microtome capable of cutting MMA emebdded bone specimens. Can anyone reccomend a good model, manufacturer, supplier or know of a good used machine ?   Many thanks.   Adam ___ Histonet mailing list

[Histonet] RE: Cassette Marking

Hi All, If anyone would like to try a free sample of the KP Lab marker for cassette, slide, or general purpose labeling, feel free to contact me and I will be more than happy to send you one. It's a win-win situation!! Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt

Re: [Histonet] chicken EGFP aby

I've had good luck using Abcam's chicken polyclonal: http://www.abcam.com/GFP-antibody-ab13970.html I use a directly conjugated secondary, and the staining is usually pretty bright. But then again, my GFP expression is high. Adam On Thu, Oct 7, 2010 at 7:44 AM, Susan Travers traver...@osu.edu

Re: [Histonet] Bone IHC

time. Adam On Thu, Oct 7, 2010 at 2:20 PM, Liz Chlipala l...@premierlab.com wrote: We lower the temp of retrieval to 70C for 2 hours and have good success with that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office

Re: [Histonet] Tyramide

conjugated antibody 6) Add biotinyl tyramide reagent 7) Add SA-HRP 8) Add DAB I've never tried it, but I'd expect it won't amplify as well as my method. However, I see no reason why it shouldn't work. Good luck, Adam On Tue, Sep 28, 2010 at 11:13 AM, sgoe...@xbiotech.com wrote: Hello world

Re: [Histonet] Ventana Renaissance Containers

Hi Ricky, We may have what you need, we have multiple sizes of containers which we can either send empty or can fill for you whichever you prefer. Check out the site if you would like at www.sensorhealth.com Hope that helps. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt

[Histonet] Microscope Slide stamp

. Feel free to check us out at www.sensorhealth.com http://www.sensorhealth.com/ for more details and let us know if you would like to try a free sample. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519

Re: [Histonet] IHC

I always leave my primary antibodies on overnight at 4C. I've seen some protocols for tricky antigens where people leave it on for days. I wouldn't leave it a room temperature though. Go home and enjoy your Friday, Adam On Fri, May 14, 2010 at 4:37 PM, sgoe...@xbiotech.com wrote: Can you

Re: [Histonet] IF Doublestaining

anecdotal reports of two primaries or two secondaries forming immune complexes when mixed together, but I've never really had that problem. Good luck, Adam On Thu, May 13, 2010 at 1:00 PM, Igor Deyneko igor.deyn...@gmail.comwrote: Hello Everyone! I'm planning to try some IF co-staining with 2

Re: [Histonet] list responses

Rather than doing a simple reply, choose reply all. Adam On Wed, Apr 14, 2010 at 2:28 PM, Carrie Disbrow dis...@shands.ufl.eduwrote: Hi We kept wondering why our responses to the discussion are not seen on the daily list. We then realized the response must be going directly to the person

[Histonet] Troubleshooting IHC

, and the background was still really bad. I see real staining sometimes, but I need to quantify this staining using histomorphometry, so I really need clean staining. Any ideas? The only thing I can think of is that 1:200 is just at the limit of titration that gives too much background. Thanks, Adam

[Histonet] Re: Troubleshooting IHC

Yes. I block in 3% H2O2, followed by protein block (it's a mysterious buffer called TNB that comes with the tyramide amplification kit), and then avidin/biotin. Adam On Thu, Mar 25, 2010 at 2:25 PM, Margaryan, Naira nmargar...@childrensmemorial.org wrote: Hi Adam, How do you block? I

Re: [Histonet] Anti-human abs work on porcine tissue

ask nicely, most companies will tell you what antigen or antigen fragment they used, but most don't actively advertise this. Adam On Wed, Mar 17, 2010 at 8:15 PM, RICKY MATHIS cmmath...@bellsouth.netwrote: I work with some porcine tissues and it is sometimes difficult to find antibodies

Re: [Histonet] Unsubscribe please

I think a lot of grief could be saved if the managers of HistoNet added the following four words at the bottom of each e-mail. Who would I need to contact to make such a suggestion? http://lists.utsouthwestern.edu/mailman/listinfo/histonet to change subscription preferences Adam On Tue, Mar 16

Re: [Histonet] Double labeling with antibodies that need different fixatives

http://www.ncbi.nlm.nih.gov/pubmed/19052548 Just to be clear, they used zinc buffered formalin, which isn't the same thing as zinc fixative. Adam On Mon, Feb 22, 2010 at 8:41 PM, Phebe Verbrugghe pverbrug...@meddent.uwa.edu.au wrote: Hi all, I would like to do an immunofluorescent double

Re: [Histonet] Double labeling with antibodies that need different fixatives

commercially available zinc buffered formalin which comes in gallon jugs for around $50. We don't do any special processing. We plop our sample (bones) in zinc formalin overnight at 4C, decalcify in EDTA or formic acid (only necessary for bones), and then embed just like any other sample. Adam On Mon

Re: [Histonet] IF staining on peritoneal macrophages

until there is no obvious liquid, before I added the fixative. I managed to get usable (but not ideal) cytospins from as few as 1000 cells. Let me know if it works, Adam On Fri, Feb 19, 2010 at 9:47 AM, Mauricio Avigdor bitesizell...@gmail.comwrote: Thank you Adam and Jay for your replies

Re: [Histonet] IF staining on peritoneal macrophages

to stick to the slides by circling the cells with a PAP pen and then just putting fixative inside PAP while the slides are lying horizontally. Adam On Thu, Feb 18, 2010 at 5:00 PM, Mauricio Avigdor bitesizell...@gmail.comwrote: Greetings all, I am trying to do immunofluorescence on peritoneal

Re: [Histonet] Alternatives to BioQuant for Bone Histomorphometry

these vectors each time, and if you forgot to do that, you could do a whole bunch of work and have BioQuant either compute the parameters completely wrong or simply discard what you did. Adam On Mon, Feb 8, 2010 at 6:45 AM, Jack Ratliff ratliffj...@hotmail.comwrote: Adam, What stain are you using

Re: [Histonet] TRAP Assay and IHC

down rapidly so the entire section should get pressed onto the slide as quickly as possible. If you go slowly and allow the section to pull itself up onto the slide in increments, you'll often get falling off. I hope that helps, Adam On Thu, Feb 4, 2010 at 9:19 AM, Sherwood, Margaret msherw

[Histonet] Lineage tracing in bone

appreciate your suggestions. There is a small chance that I may get access to a Cryojane at some point in the future, and I would also welcome comments on how feasible this would be using that system. Thanks, Adam ___ Histonet mailing list Histonet

Re: [Histonet] 0.1 M PB 0.01M PBS

I've used 1X PBS for everything and it seems to work. Adam On Sun, Jan 17, 2010 at 8:41 PM, TF ti...@foxmail.com wrote: We use 0.1 PB for tissue fixation (4% PFA), cryo-preservation (30% sucrose) then go 0.01 PBS for IHC though these are routines here, I wonder anyone can specify on the use

Re: [Histonet] Counterstain for fluorescent tissue

software such as Photoshop. Adam On Sat, Jan 9, 2010 at 12:46 AM, John Kiernan jkier...@uwo.ca wrote: Dear Nick Evans, First: Say who and where you are, and who is in charge of your experiments with mice. Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each

Re: [Histonet] mouse Cytokine ICC/IHC protocol help.

cells. Since it's not easy, our lab tries to avoid doing this type of delicate work and instead do ELISAs and intracellular flow cytometry. Adam On Mon, Jan 4, 2010 at 12:41 PM, Jamie E Erickson jamie.erick...@abbott.com wrote: Hi All, Happy New Year... As the new year is upon us I and my

[Histonet] Directly conjugated secondaries versus biotinylated secondaries

the filters for it. 5) This is all a fluke and I screwed something up Any suggestions on how to troubleshoot this are welcome. Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: Akemi - Urgent!

links in it or dial any phone number contained within. Instead, look up the company's contact information (in a phone book or via a search engine) and use the publicly available contact information. Adam On Thu, Nov 12, 2009 at 11:04 AM, Rene J Buesa rjbu...@yahoo.com wrote: Akemi

Re: [Histonet] Green Fluorescent Protein

. Adam On Tue, Oct 27, 2009 at 8:50 AM, Paula Pierce cont...@excaliburpathology.com wrote: Hello, I routinely use Invitrogen's Rabbit anti-GFP on mouse and rat FFPE tissue. Paula From: Melanie Black melanie.bl...@uct.ac.za To: histonet

[Histonet] Isotype background

, whole molecule from Jackson. Some people have suggested that I just do away with isotypes altogether and use a no primary control instead. I think there is some merit to this idea, but I still think my issue might be indicative of a larger technical problem in my staining protocol. Thanks, Adam

[Histonet] Going from immmunohistochemistry to immunofluorescence

together, I would welcome those suggestions as well. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Staining with two primary antibodies from same host

this during your long weekend, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Re: anti-GFP antibody

, and 1:2000. I should've titered it even further down. Thanks for all your help, Adam On Thu, Aug 20, 2009 at 1:38 PM, Hobbs, Carl carl.ho...@kcl.ac.uk wrote: I am sure someone has a cunning combination! I use Abcam's ab13970 chicken anti GFP in Pwax sections after HIER. For me, it is very

Re: [Histonet] uneven alternating sections on cryostat

and causes the chuck to wobble. For some reason, this causes your problem. Now I make sure that everything is tightly secured, and I rarely have this problem. Good luck, Adam On Thu, Aug 27, 2009 at 4:47 PM, Nathan Cramer natec...@gmail.com wrote: Thanks, Robyn... I'll make sure the holder

[Histonet] Cleaning oil off objectives

? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] anti-GFP antibody

-on-mouse issue. Alternatively, I could buy two antibodies (rabbit and goat) and use them in the respective assays. Or is there another way that's non-obvious? Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] 4F:1G

I'm new to the field of histology and want to use 4F:1G to fix fish ovaries for later histological examination under light microscopy. Following fixation, I plan to rinse the ovary samples in an ethanol series (50-95%). First, what is the minimum time needed to fix 10 g of tissue? Second, I

[Histonet] Cryoprotection Issues with Unfixed Mouse Hearts

Does anyone have any suggestions or protocols for cryoprotecting unfixed mouse heart tissue? Some of the ones I have been cryosectioning lately have been damaged, I think because of improper cryoprotection. Thanks! Adam baza0...@umn.edu

[Histonet] UC Berkeley students in need of help

. There are 11 short questions- please help if you have time. Link to online survey: http://www.surveymonkey.com/s.aspx?sm=X3ssptgNq4oh7o0vwy1gCg_3d_3d Please contact me with any questions. Thanks, Adam Anthony adam_anth...@mba.berkeley.edumailto:adam_anth...@mba.berkeley.edu

[Histonet] HIF-1alpha or Ref-1 immunohistochemistry on mouse FFPE tissues

Does anyone have any suggestions or a working immunofluorescence protocol for staining mouse FFPE tissues with HIF-1alpha rabbit polyclonal or Ref-1 rabbit polyclonal? Both from Santa Cruz. Thank you, Adam Adam Bazama baza0...@umn.edu Lillehei Heart Institute Histology Microscopy

[Histonet] Cutting fresh-frozen brains from 1 week old rat pups

cohort of animals with PFA and then a 30% sucrose step to see if that helps, but I was hoping that someone out there would have some tips on cutting these immature brains. Thanks, Adam. Adam Galle, Neuropharmacology and Brain Injury Lab Department of Pharmacology School of Medical Sciences

[Histonet] sirius red connective tissue stain[Scanned]

Hi Dave, I noticed that you posted a request in march of 2004 on histonet for a Sirius red fast green staining protocol. If you do have a protocol, would you mind sending it to me. I have had a hard time finding one to compare to mine and my lack luster results. Thank you, Adam