[Histonet] Santa Cruz Antibodies

Hello Histonet, I'm hoping someone will be able to clear something up for me. I've seen several articles in the news recently regarding the revocation of Santa Cruz' animal dealer license, the closing of their research lab, and no longer being allowed to sell antibodies past December 31 of this ye

[Histonet] Samples not staining with Hematoxylin or primary

Thanks so much to everyone who responded! A couple people have suggested that this issue may be due to a disruption to the charge on the slide (due to high temp baking, water bath additives, etc) which causes a hydrophobic barrier to form and prevents reagent from reaching the tissue. This sounds v

[Histonet] IHC antibody/assay recommendations for IL-4, IL-13, IL-4R, and IL-13Ra1

Just wondering if anyone has had good luck with any of these antibodies (IL-4, IL-13, IL-4R, and IL-13Ra1) and would be willing to share vendor info and/or assay conditions they've tested? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/

[Histonet] Samples not staining with Hematoxylin or primary

Hello Histonetters, I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with ei

[Histonet] Suggestions for staining bacteria in FFPE decaled growth plate

Happy Friday, Histonetters! I have a puzzle that I could really use your expert knowledge on this morning. One of our pathologists has a decalcified FFPE mouse bone embedded longitudinally and has found some bacteria located in the growth plate. The bacteria has been confirmed with fluorescence, b

[Histonet] HTL Career Path

Hello Histonet, I have been following this listserve for a couple years now and have found it to be a very interesting and helpful forum for sharing ideas and information among individuals with varied career histories in the histopath field. I sort of fell into histology work while working on my M

[Histonet] Air bubbles in Coverslipper

Hi Histonetters! We have a Leica CV5030 coverslipper in our lab and we regularly (~ once a week or so) have trouble with large air bubbles showing up in a significant number of our slides. We have flushed the hose from the mounting medium through the needle with xylenes which sometimes works to c

[Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain

Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour