[Histonet] Re IHC help

2024-08-08 Thread Catherine Anne Heyward via Histonet
Hi, If HIER at 90 degrees C is causing tissue lifting, you could try a lower temperature for longer. I was recommended 60 degrees overnight for bone/cartilage and have had far fewer problems with this gentler treatment. Perhaps this would suit the skin samples too. Best wishes, Cath Catheri

Re: [Histonet] Jores fluid - Histonet Digest, Vol 238, Issue 10

2023-09-21 Thread Anne van Binsbergen via Histonet
Hello gang Jores has several references found in a Google search, including a post right here on the Histonet from 2002. It contains Chloral Hydrate which is very difficult to find. Do a search and have a look. Several suggestions. AnnieinArabia (now retired in Africa) Sent from my iPhone

Re: [Histonet] Testing for hires

2022-11-16 Thread Anne Murvosh via Histonet
Melt down old blocks, have them embed and cut them. Evaluate their time and technique. You could also do a working interview where a tech shows them around but has them cut and embed a few current tissues while watching. Thanks Anne Anne Murvosh Histology Technician

[Histonet] Resumes for laboratory

2022-10-17 Thread Anne Murvosh via Histonet
I wanted to update my resume just to have in case of emergency. I have not done one in many years, and I know some formatting has changed. Does anyone have a good template that relates to the laboratory? Thanks Anne Anne Murvosh Histology Technician Three

[Histonet] MOHS scheduling

2022-09-12 Thread Anne Murvosh via Histonet
I was wondering, for those doing Mohs cases, how far apart you schedule your patients, and if you do them throughout the day or just in am or pm. Thanks Anne Anne Murvosh Histology Technician Three Locations to Serve YOU! Spokane Valley

[Histonet] Small processors

2022-08-02 Thread Anne Murvosh via Histonet
. Any thoughts. Thanks Anne Murvosh HT HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual

Re: [Histonet] Cryostat anti-roll plate

2022-05-25 Thread Anne Murvosh via Histonet
adjusting. Thanks Anne -Original Message- From: Ken Marzinsky via Histonet Sent: Tuesday, May 24, 2022 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat anti-roll plate Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work

[Histonet] Once-a-month job opportunity

2022-03-01 Thread Anne Murvosh via Histonet
pm.Two years' experience preferable. Thanks Anne Murvosh H. Please Contact: Advanced Dermatology & Skin Surgery, in connection with United Derm Partners Mohs TECHNICIAN-WALLA WALLA, Washington 3RD Friday of every month Please contact: Anne Marie Poole apo...@advancederm.net&l

[Histonet] Stain line order

2021-10-26 Thread Anne Murvosh via Histonet
luing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne Anne Murvosh Histology Technician This electronic trans

[Histonet] Mohs Tech Wage?

2021-09-22 Thread Anne Murvosh via Histonet
We are trying to hire a part-time Mohs technician in Washington state that is a train on the job. I feel like the wage is too low, what are beginning Mohs techs making if they are not a histology tech? Thanks Anne HIPAA Confidentiality Notice: The information and documents accompanying this e

[Histonet] Formalin question

2021-02-23 Thread Anne Murvosh via Histonet
I was wondering if 10% formalin needed to be kept in a flammable cabinet. We never have before, but I wondered if we were doing it wrong or if regulations have changed. Thanks Anne Murvosh HT. HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain

[Histonet] Tissue processors.

2020-09-17 Thread Anne van Binsbergen via Histonet
Hi Histonetters Your best bet is a VIP6. Don’t think the VIP5 is still being manufactured. If it is then grab one. I had 3 VIP5s. The one is still in service after 16 years. A trusty workhorse. Easy to use. Sakura instruments are just amazing. Greetings from Cape Town. Anne Sent from my

[Histonet] Cryostats for Mohs specimens

2020-09-02 Thread Anne Murvosh via Histonet
nd be able to get a lot of tissue on each slide, sometimes the components of the machine get in the way of good tissue pick-up. Thanks Anne Murvosh HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/lis

[Histonet] Mart-1 rapid test for Mohs specimens

2020-08-13 Thread Anne Murvosh via Histonet
t- 1 stain? Thanks Anne Murvosh (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Film coverslip vs glass coverslip

2020-08-05 Thread Anne van Binsbergen via Histonet
Hi all I have used Sakura film coverslippers in several different facilities since 1997. In the ‘early days’ there were some issues with slip detachment but those were smartly sorted out by the manufacturers. 1) Film coverslips dry really fast - no clumps of stuck together slides to soak for

[Histonet] Frozen tissue

2020-07-09 Thread Anne Murvosh via Histonet
or do I need to refrigerate or freeze? Also how long do they last, I can't remember the protocol. Thanks Anne HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Xylene recycle

2020-06-23 Thread Anne Murvosh via Histonet
I'm looking to switch from a disposal pick-up to recycling of Xylene. Can anyone tell me some companies that offer a recycling unit. The catch is we don't have very much so I need something for a small lab. Thanks Anne ___ Histonet ma

[Histonet] Processor

2020-04-13 Thread Anne Murvosh via Histonet
nging out completely? Does it lose its stability? Thanks Anne Murvosh (HT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Formaldehyde test kit question

2020-02-04 Thread Anne Murvosh via Histonet
he instructions say to add 10 drops of a sodium hydroxide solution to the solution being tested, wouldn't that change the Ph and make the results not as accurate. This kit is expensive if regular pH strips are better, let me know that too. Thanks

[Histonet] Xylene and Formalin monitoring

2019-12-11 Thread Anne Murvosh via Histonet
Just need a refresher on formalin and xylene monitoring regulations. Do we still have to do a 15 minute and 8 hour testing on both xylene and formalin, or can we just do the 8 hour. I wasn't sure if the regulations have changed or not. Thanks

[Histonet] Yearly review

2019-09-19 Thread Anne Murvosh via Histonet
Hey everyone, does the yearly employee competency assessments need to be kept for just 2 years or for the entire time an employee is there. I have some old ones I want to toss, but I just wanted to be sure. Thanks Anne ___ Histonet mailing list

Re: [Histonet] Help to interview new employees for the first time

2019-09-13 Thread Anne Murvosh via Histonet
I'm not sure if this is an option, but we used to melt down old blocks and make the HT embed and cut them. This showed us how good and quick they were and if they actually new what they were doing. Anne -Original Message- From: Blanca Lopez via Histonet Sent: Thursday, Septemb

[Histonet] Grossing large ellipse melanomas

2019-01-08 Thread Anne Murvosh via Histonet
suggestions or links to grossing techniques would be appreciated. Also, any ideas on better cutting utensils for these. Thanks Anne Murvosh HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] IDH1 source

2018-12-15 Thread Anne van Binsbergen via Histonet
everyone! Anne Anne S van Binsbergen Supervisor, Histology Laboratory Pathology and Laboratory Medicine Sheikh Khalifa Medical City Abu Dhabi United Arab Emirates Sent from my iPhone > On Dec 15, 2018, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mai

[Histonet] Gout specimens

2018-11-08 Thread Anne Murvosh via Histonet
I haven't done a gout specimen in years, I remember the processing part, not remembering the Staining part. Could someone please send me your gout protocol? Thanks Anne Murvosh HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Kwik Diff

2018-09-07 Thread Anne van Binsbergen via Histonet
Hi Histonetters Try the Kwik Diff from Thermo Shandon. We have been using it for as long as I can remember. I’m sure there must be suppliers in the USA. Annie in Arabia Sent from my iPhone > On 07 Sep 2018, at 18:00, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing l

histonet@lists.utsouthwestern.edu

2018-04-25 Thread Anne van Binsbergen via Histonet
Tim We use Cerner Millennium and are considering adding AB&T. Apart from the modifications and tweaking of the actual system, my concern with a very tight space issue, is the need to have hardware set up at each grossing (2) and microtome station (4) and the obvious addition expense related to t

[Histonet] Processors

2018-01-30 Thread Anne Murvosh via Histonet
Can anyone recommend a processor for a smaller lab. I need one for about 150 to 200 specimens. It can be a floor model or tabletop preferably a used model. Processing skin specimens only, I currently have a leica TP 1020. Venders welcome to reply. Thanks for your input, Anne

[Histonet] Temp employees

2017-09-12 Thread Anne Murvosh via Histonet
Does anyone know of a company that provides temp employees for a Mohs technician? We need to cover someone for a little over a month in February or March of next year. Thanks Anne Murvosh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] CEU's

2017-06-05 Thread Anne Murvosh via Histonet
I was wondering if college classed not related to histology counted for continuing education. I'm going back to school and although I think science classes would count, I wasn't sure about a math or English class. Does anyone know for sure. T

[Histonet] Slide disposal

2017-05-01 Thread Anne Murvosh via Histonet
spose of them properly. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Pathology Ladder System

2016-08-09 Thread Anne Murvosh via Histonet
If you don't already have it you may want to offer a shift differential for very early or late shifts and weekends. This makes it a little easier to hire for and keep people on the harder shifts. Anne -Original Message- From: Steve via Histonet [mailto:histonet@lists.utsouthwester

[Histonet] Formaldehyde and Organic vapor monitors.

2016-07-26 Thread Anne van Binsbergen via Histonet
and I will email you screenshots of the the exact ones we use. Have a fabulous day. Anne Ps Of course we do have excellent ventilation/extraction. It helps Sent from my iPhone > On 26 Jul 2016, at 9:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list

[Histonet] Spill kits

2016-06-14 Thread Anne Murvosh via Histonet
Do spill kits need to be out where anyone can see them, or can I put it in a cupboard with a sign on the door? I need to move mine and there's very little room. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Hoods

2016-05-24 Thread Anne Murvosh via Histonet
We had an inspection from the fire department recently and they said we need to have the exhaust from the fume hood checked annually. Does anyone know who does that? The people that do our P.M. on our machines say they don't, so who would? Thanks

Re: [Histonet] PAS Stain

2016-05-05 Thread Anne Murvosh via Histonet
psied himself and treated it. There's a non-uniform method that saved a lot of suffering. Bravo we crazy scientists. Anne -Original Message- From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet@lists.utsouthw

Re: [Histonet] PAS Stain

2016-05-05 Thread Anne Murvosh via Histonet
d the spit method at the same time and have the doctor pick the best. A fun experiment Anne -Original Message- From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 11:36 AM To: koelli...@comcast.net Cc: Histonet@lists.utsouthwester

[Histonet] Height regulations

2016-01-04 Thread Anne Murvosh via Histonet
Putting in some lower shelving for storage and I can't remember the height regulation for keeping stuff off the floor. I tried to google it, it must be buried in some government doc. Please Help? Thanks Anne ___ Histonet mailing list His

[Histonet] Auto specials

2015-12-29 Thread Anne Van Binsbergen via Histonet
. Consistently good results. The system is user friendly and the staining protocols are easy to tweak and customize. Highly recommended. Regards Anne Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Off topic posts

2015-12-20 Thread Anne Van Binsbergen via Histonet
Dear Lester Raff MD Yes some of us do visit the Histonet over weekends. Your posts are so annoying and self-serving. Please go away! Thanks Annieinarabia Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.uts

Re: [Histonet] Histonet Digest, Vol 145, Issue 7

2015-12-07 Thread Anne Van Binsbergen via Histonet
Hi all Anyone successfully using IDH1? Have been asked to source but am having a hard time finding a supplier. Anyone? Ta Anne Sent from my iPhone > On Dec 7, 2015, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >

[Histonet] IHC breast markers: changing source of NBF

2015-05-10 Thread Anne Van Binsbergen
Greetings esteemed Histonetters We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. We also strictly adhere t

[Histonet] Home made IHC breast markers: home made NBF vs Commercially prepared

2015-05-03 Thread Anne Van Binsbergen
Greetings esteemed Histonetters We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. We also strictly adhere t

[Histonet] RE: NY State Histology License

2015-04-24 Thread Anne Murvosh
There is a grandfather clause if you were a histotech by april 24.1995. I asked ClIA recently when they came to inspect me, as I fell into this category but didn't know if you had to have the licence by then or just have been performing high complexity test at that time (my licence was later th

RE: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread Anne Murvosh
ry its going to be a lot of trial and error. Maybe you can call the previous tech on how they did it there. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April

[Histonet] RE: cleaning glassware

2015-04-02 Thread Anne Murvosh
I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff

[Histonet] RE: Floor scraper

2015-03-04 Thread Anne Murvosh
just a paint scraper at your local hardware store. Some people attach it to a broom handle so they don't have bend down. They may even have a long one. Anne From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwester

[Histonet] RE: embedding

2015-01-21 Thread Anne Murvosh
a bit longer to embed cause the bottom takes longer to warm up so it's a trade off for time. Fun experiment though! Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent:

RE: [Histonet] marking small samples

2015-01-15 Thread Anne Murvosh
I mark shaves and punches with .5% Toludine blue it won't color the skin but will color the rest. And best of all it doesn't show up in any staining its purely for identifying your tissue for embedding purposes. Anne -Original Message- From: histonet-boun...@lists.utsouthw

[Histonet] Dako Artisan Warthin Starry

2014-12-23 Thread Anne Van Binsbergen
We do them successfully all the time. Without pretreatment. AnnieinArabia Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Animal Tissue/Human Tissue

2014-11-04 Thread Anne Murvosh
My first job was at a lab that did both human and animal (till a case got mixed up) the only difference I found was animals were fattier and hairier. Same microtome. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] New Artisan Gram stain kits

2014-09-24 Thread Anne
Hello there you awesome group of histo gurus... We have been running the new improved Artisan Gram kits. They say to use the standard on board staining protocol. This gives us no gram+ orgs. We reset the CV to its max. Still no blue. We used the same new kit reagents manually with the same instr

RE: [Histonet] Mohs

2014-09-24 Thread Anne Murvosh
each time. Time consuming yes. But I find freezing and let sitting I don't fight with it as much when cutting. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of michele carr Sent: Tuesday, Septemb

RE: [Histonet] Practice tissue?

2014-06-04 Thread Anne Murvosh
Contact your local labs and Hospitals, you could just use some already embedded and cut tissue that is about to be disposed of as long as there is no patient info on the blocks it shouldn't be a problem. Anne -Original Message- From: histonet-boun...@lists.utsouthwester

RE: [Histonet] Microtome

2014-06-03 Thread Anne Murvosh
Most companies will let you test run their newest microtomes. Let them come to you and test for a week or so than pick the one you like best. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia

RE: [Histonet] (no subject)

2014-05-30 Thread Anne Murvosh
I just got back from the Mohs conference. The Biocare people have the stain and told me they would come out to help work it up. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bethany Sent: Thursday

RE: [Histonet] antibody vials

2014-05-20 Thread Anne Murvosh
We got some plexiglass dividers at the "Container Store" if you have one near or check online. Can't remember what they were called but they had different sized square compartments that fit various venders antibodies. Anne -Original Message- From

RE: [Histonet] company for antibody HSV(cocktail) on paraffin section

2014-04-28 Thread Anne Murvosh
Biocare has a herpes I&II mix if that's what you're looking for. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 28, 2014 5:27 A

RE: [Histonet] Ventana Vantage and H&E stainer

2014-02-12 Thread Anne Murvosh
spital in Spokane Wa Has the complete system so you could give them a call. 509-474-4108 Thanks Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, February 12, 2014 10

RE: [Histonet] Safety items

2014-01-07 Thread Anne Murvosh
Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] Paperwork

2014-01-06 Thread Anne Murvosh
OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2 years with Clia, like you do CAP specifically Temperature and maintenance charts. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] Helicobacter helmani -- help?

2013-12-04 Thread Anne Murvosh
, it has a lot of papers written on it that might help. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, December 04, 2013 12:19 PM To: histonet@lists.utsouthwestern.e

RE: [Histonet] Rate of microtome injuries in Histotechnology schools.

2013-12-02 Thread Anne Murvosh
If anyone wants to cut down on cutting injuries. Teach the new techs to use two forceps instead of your fingers. I'm too old to change but I know a tech who cuts this way and has never had an injury. It works really well on automated microtomes. Anne -Original Message- From: his

RE: [Histonet] human lymph nodes from a cadaver

2013-10-01 Thread Scanlan, Megan Anne
I Wellman Center for Photomedicine Massachusetts General Hospital 50 Blossom Street, EDW 214 Boston, MA 02114 617-726-6983 (Lab) 726-1206 (fax) -Original Message- From: Britton, Josette C [mailto:jcbrit...@cheshire-med.com] Sent: Tuesday, October 01, 2013 7:41 AM To: Scanlan, Megan Anne S

[Histonet] human lymph nodes from a cadaver

2013-09-30 Thread Scanlan, Megan Anne
Hi all, Does anyone have experience cutting human lymph nodes from a cadaver in paraffin blocks? I am having quite a lot of trouble getting good sections, even using a brand new blade the sections end up with multiple blade marks streaking through the tissue and paraffin. I've contacted the

[Histonet] Flammable Liquid

2013-09-17 Thread Anne Murvosh
Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] Re: Histonet Digest, Vol 118, Issue 24

2013-09-12 Thread Anne
My goodness ignorance abounds...Rene J is one of the most respected voices on this forum. And 'she' is actually a 'he' you dimwits. I would take his advice above almost anyone on this forum. You youngsters should try to emulate rather than denigrate. Rene you rock!!! Annieinarabia Sent from

[Histonet] Re: Histonet Digest, Vol 118, Issue 24

2013-09-12 Thread Anne
Choose the FILM coverslipper. Sakura and DAKO over Leica anytime. Annieinarabia Sent from my iPhone On 12 Sep 2013, at 9:02 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscrib

[Histonet] RE: immunohistochemistry help please

2013-08-09 Thread Tarantelli, Rebecca Anne
67-6600 -Office (724) 494-6166 -Cell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tarantelli, Rebecca Anne Sent: Friday, August 02, 2013 10:30 AM To: Sebree Linda A; histonet@lists.utsouthwestern.edu Sub

[Histonet] RE: immunohistochemistry help please

2013-08-02 Thread Tarantelli, Rebecca Anne
-Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, August 01, 2013 3:50 PM To: Tarantelli, Rebecca Anne; histonet@lists.utsouthwestern.edu Subject: RE: immunohistochemistry help please OK, so I would be very suspect of your xylene. Has anything changed, i.e

RE: [Histonet] immunohistochemistry help please

2013-08-02 Thread Tarantelli, Rebecca Anne
...@gmx.at] Sent: Friday, August 02, 2013 7:40 AM To: Tarantelli, Rebecca Anne Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] immunohistochemistry help please Are all slides from the same tissue sample with the same pretreatment? I can't believe, that you don't have any hematoxyli

[Histonet] RE: immunohistochemistry help please

2013-08-01 Thread Tarantelli, Rebecca Anne
ge- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, August 01, 2013 3:36 PM To: Tarantelli, Rebecca Anne; histonet@lists.utsouthwestern.edu Subject: RE: immunohistochemistry help please To test your deparaffinization Rebecca, I'd try staining a slide with something

[Histonet] immunohistochemistry help please

2013-08-01 Thread Tarantelli, Rebecca Anne
Hi all, I am new to this forum, but a friend recommended I send my issue out to you for help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC) using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked perfectly. Three days later, after 13 beautiful stai

[Histonet] Merry Christmas

2012-12-24 Thread Anne van Binsbergen
Annie in Africa aka ArabianAnnie is back and wishes you all a very Merry Christmas and festive New Year - Happy Holidays -- Anne van Binsbergen Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] unsubscribe

2012-07-24 Thread Lewin, Anne
Unsubscribe, please This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended rec

[Histonet] tissue arrays

2010-08-02 Thread Anne van Binsbergen
se thanks AnnieinArabia -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] assisting with patient bone marrows

2010-07-15 Thread Anne van Binsbergen
nfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histo

Re: [Histonet] (no subject)

2010-04-16 Thread Anne van Binsbergen
take the poor animal to a vet - if it is a BCC or an SCC (which are both CANCER by the way..and it can spread) then it needs proper treatment would you treat your child like this - I hope not and just by the waythis is not a pharmacy listserver On 16 April 2010 20:04, James Ball wrote: >

Re: [Histonet] BREAST IMPLANTS AND HARDWARE

2010-04-07 Thread Anne van Binsbergen
t; disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/hi

Re: [Histonet] Is my Leica CM 1850 cryostat haunted?

2010-03-30 Thread Anne van Binsbergen
ist > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] number of slides

2010-03-25 Thread Anne van Binsbergen
in > error, please notify the sender at the electronic mail address noted above > and destroy all copies of this communication and any attachments. Thank you > for your cooperation. > > ___ &

Re: [Histonet] Thermo Slide and Cassette printers

2010-03-18 Thread Anne van Binsbergen
western.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen

[Histonet] Block patient IDs

2010-03-12 Thread Anne van Binsbergen
-- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] RE: CPT code 88313 on non-gynecological cases

2010-02-04 Thread Anne van Binsbergen
edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Re: Sakura iDent

2010-01-13 Thread Anne van Binsbergen
t-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van > Binsbergen > Sent: Wednesday, January 13, 2010 1:16 AM > To: Denise Van Eaton > Cc: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Sakura iDent > > Hi De

[Histonet] Re: Sakura iDent

2010-01-13 Thread Anne van Binsbergen
me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton > Hi Anne, > > We have been

Re: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome

2009-12-31 Thread Anne van Binsbergen
et > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Hello fellow Histo's , 2 questions for you

2009-12-24 Thread Anne van Binsbergen
//lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

histonet@lists.utsouthwestern.edu

2009-12-10 Thread Anne van Binsbergen
; - 80% EtOH - 1-2min > > - Eosin - 2 min > > - 95% EtOH I - 10sec > > - 95% EtOH II - 10sec > > - 100% EtOH I - 10sec > > - 100% EtOH II - 10sec > > - Xylene I - 2min > > - Xylene II - 2min > > > > 7. Coverslip and let dry overnight > >

Re: [Histonet] Bachelor's Degrees

2009-12-06 Thread Anne van Binsbergen
___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://list

Re: [Histonet] FORMALDEHYDE

2009-12-03 Thread Anne van Binsbergen
information without permission. If you received this > e-mail > > transmission in error, please notify the sender immediately to arrange > for > > return > > or destruction of this information. > > > > ___ > > Histonet mailing list > >

Re: [Histonet] Cerner Millenium

2009-12-02 Thread Anne van Binsbergen
> Skeptic(tm) technology powered by MessageLabs. For more information on MCI's > Managed Email Content Service, visit http://www.mci.com. > __ > > ___ > Histone

[Histonet] Cassette labelling

2009-11-29 Thread Anne van Binsbergen
Tissue-Tek xylene substitute - my xylene is made by Fisher I would love to know if anyone else has had similar issues Yes, Rene, this is the diehard number 1 Sakura fan having issues with a Sakura product!!! hopefully yours (pun intended) AbuDhabiAnnie -- Anne van Binsbergen (Hope) Abu Dhabi UAE

Re: [Histonet] Sakura Embedding

2009-11-25 Thread Anne van Binsbergen
list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Control for Spirochete

2009-11-17 Thread Morin, Mary Anne
stain didn't work. I'm interested in either paraffin blocks or a company that uses Plus slides. Thanks for your help. Marie Anne Morin HT. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fa

RE: [Histonet] B5 alternate

2009-11-13 Thread Morin, Mary Anne
is 1-800-635-4477. Have A Good One!! Marie Anne Morin H.T. ASCP Path Tech Specialist Histology Lab Rhode Island Hospital Office 444-7196 Pager 350-7384 Fax 444-8514 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on

Re: [Histonet] B5 alternate

2009-11-13 Thread Anne van Binsbergen
> > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list >

Re: [Histonet] Procedure for manual C4D immunofluorescence staining

2009-10-15 Thread Anne van Binsbergen
66-6580Fax 414-266-2779 > afos...@chw.org > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >

Re: [Histonet] Automated H&E stainers

2009-10-09 Thread Anne van Binsbergen
rror, please notify us immediately by reply e-mail and > then delete it from your system. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi U

Re: [Histonet] Re: Brain frozen sections

2009-10-07 Thread Anne van Binsbergen
be frezzing artefact I will pass the message to them and then I will run and hide!! LOL ;D thanks greetings from the desert Anne 2009/10/7 Tim Wheelock > Hi: > > There is one thing you might try to make frozen brain sections more than > acceptable. > You can freeze the tissue in

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