Hi,
If HIER at 90 degrees C is causing tissue lifting, you could try a lower
temperature for longer. I was recommended 60 degrees overnight for
bone/cartilage and have had far fewer problems with this gentler treatment.
Perhaps this would suit the skin samples too.
Best wishes,
Cath
Catheri
Hello gang
Jores has several references found in a Google search, including a post right
here on the Histonet from 2002.
It contains Chloral Hydrate which is very difficult to find.
Do a search and have a look. Several suggestions.
AnnieinArabia (now retired in Africa)
Sent from my iPhone
Melt down old blocks, have them embed and cut them. Evaluate their time and
technique. You could also do a working interview where a tech shows them around
but has them cut and embed a few current tissues while watching. Thanks Anne
Anne Murvosh
Histology Technician
I wanted to update my resume just to have in case of emergency. I have not done
one in many years, and I know some formatting has changed. Does anyone have a
good template that relates to the laboratory? Thanks Anne
Anne Murvosh
Histology Technician
Three
I was wondering, for those doing Mohs cases, how far apart you schedule your
patients, and if you do them throughout the day or just in am or pm. Thanks Anne
Anne Murvosh
Histology Technician
Three Locations to Serve YOU!
Spokane Valley
. Any thoughts.
Thanks Anne Murvosh HT
HIPAA Confidentiality Notice: The information and documents accompanying this
e-mail may contain confidential information that is legally privileged and
protected by federal and state law. The information is intended for use only by
the entity or individual
adjusting. Thanks Anne
-Original Message-
From: Ken Marzinsky via Histonet
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate
Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat?
Does it work
pm.Two years'
experience preferable. Thanks Anne Murvosh H.
Please Contact:
Advanced Dermatology & Skin Surgery, in connection with United Derm Partners
Mohs TECHNICIAN-WALLA WALLA, Washington
3RD Friday of every month
Please contact: Anne Marie Poole
apo...@advancederm.net&l
luing, rinse, clearing. I
don't remember which is first. Actually do you need a bluing if using tap water
since that blues better then DI water? Thanks for your input, it's been 10
years since I had to think about this. Anne
Anne Murvosh
Histology Technician
This electronic trans
We are trying to hire a part-time Mohs technician in Washington state that is a
train on the job. I feel like the wage is too low, what are beginning Mohs
techs making if they are not a histology tech? Thanks Anne
HIPAA Confidentiality Notice: The information and documents accompanying this
e
I was wondering if 10% formalin needed to be kept in a flammable cabinet. We
never have before, but I wondered if we were doing it wrong or if regulations
have changed. Thanks Anne Murvosh HT.
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Hi Histonetters
Your best bet is a VIP6. Don’t think the VIP5 is still being manufactured. If
it is then grab one. I had 3 VIP5s. The one is still in service after 16 years.
A trusty workhorse. Easy to use. Sakura instruments are just amazing.
Greetings from Cape Town.
Anne
Sent from my
nd be able to get a lot of
tissue on each slide, sometimes the components of the machine get in the way of
good tissue pick-up. Thanks Anne Murvosh HT
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t- 1 stain? Thanks Anne Murvosh (HT)
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Hi all
I have used Sakura film coverslippers in several different facilities since
1997.
In the ‘early days’ there were some issues with slip detachment but those were
smartly sorted out by the manufacturers.
1) Film coverslips dry really fast - no clumps of stuck together slides to soak
for
or do I need to refrigerate or freeze?
Also how long do they last, I can't remember the protocol. Thanks Anne HT
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I'm looking to switch from a disposal pick-up to recycling of Xylene. Can
anyone tell me some companies that offer a recycling unit. The catch is we
don't have very much so I need something for a small lab. Thanks Anne
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nging out completely? Does it
lose its stability? Thanks Anne Murvosh (HT)
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he instructions say to add
10 drops of a sodium hydroxide solution to the solution being tested, wouldn't
that change the Ph and make the results not as accurate. This kit is expensive
if regular pH strips are better, let me know that too. Thanks
Just need a refresher on formalin and xylene monitoring regulations. Do we
still have to do a 15 minute and 8 hour testing on both xylene and formalin, or
can we just do the 8 hour. I wasn't sure if the regulations have changed or
not. Thanks
Hey everyone, does the yearly employee competency assessments need to be kept
for just 2 years or for the entire time an employee is there. I have some old
ones I want to toss, but I just wanted to be sure. Thanks Anne
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I'm not sure if this is an option, but we used to melt down old blocks and make
the HT embed and cut them. This showed us how good and quick they were and if
they actually new what they were doing. Anne
-Original Message-
From: Blanca Lopez via Histonet
Sent: Thursday, Septemb
suggestions or links
to grossing techniques would be appreciated. Also, any ideas on better cutting
utensils for these. Thanks Anne Murvosh HT
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everyone!
Anne
Anne S van Binsbergen
Supervisor, Histology Laboratory
Pathology and Laboratory Medicine
Sheikh Khalifa Medical City
Abu Dhabi
United Arab Emirates
Sent from my iPhone
> On Dec 15, 2018, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
>
> Send Histonet mai
I haven't done a gout specimen in years, I remember the processing part, not
remembering the Staining part. Could someone please send me your gout protocol?
Thanks Anne Murvosh HT
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Hi Histonetters
Try the Kwik Diff from Thermo Shandon. We have been using it for as long as I
can remember. I’m sure there must be suppliers in the USA.
Annie in Arabia
Sent from my iPhone
> On 07 Sep 2018, at 18:00, histonet-requ...@lists.utsouthwestern.edu wrote:
>
> Send Histonet mailing l
Tim
We use Cerner Millennium and are considering adding AB&T.
Apart from the modifications and tweaking of the actual system, my concern with
a very tight space issue, is the need to have hardware set up at each grossing
(2) and microtome station (4) and the obvious addition expense related to t
Can anyone recommend a processor for a smaller lab. I need one for about 150 to
200 specimens. It can be a floor model or tabletop preferably a used model.
Processing skin specimens only, I currently have a leica TP 1020. Venders
welcome to reply. Thanks for your input, Anne
Does anyone know of a company that provides temp employees for a Mohs
technician? We need to cover someone for a little over a month in February or
March of next year. Thanks Anne Murvosh
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I was wondering if college classed not related to histology counted for
continuing education. I'm going back to school and although I think science
classes would count, I wasn't sure about a math or English class. Does anyone
know for sure. T
spose of them
properly. Thanks Anne
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If you don't already have it you may want to offer a shift differential for
very early or late shifts and weekends.
This makes it a little easier to hire for and keep people on the harder shifts.
Anne
-Original Message-
From: Steve via Histonet [mailto:histonet@lists.utsouthwester
and I will email you screenshots
of the the exact ones we use.
Have a fabulous day.
Anne
Ps Of course we do have excellent ventilation/extraction. It helps
Sent from my iPhone
> On 26 Jul 2016, at 9:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
>
> Send Histonet mailing list
Do spill kits need to be out where anyone can see them, or can I put it in a
cupboard with a sign on the door? I need to move mine and there's very little
room. Thanks Anne
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We had an inspection from the fire department recently and they said we need to
have the exhaust from the fume hood checked annually. Does anyone know who
does that? The people that do our P.M. on our machines say they don't, so who
would? Thanks
psied himself and
treated it. There's a non-uniform method that saved a lot of suffering. Bravo
we crazy scientists. Anne
-Original Message-
From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 12:31 PM
To: histonet@lists.utsouthw
d the spit method at the
same time and have the doctor pick the best. A fun experiment Anne
-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 11:36 AM
To: koelli...@comcast.net
Cc: Histonet@lists.utsouthwester
Putting in some lower shelving for storage and I can't remember the height
regulation for keeping stuff off the floor. I tried to google it, it must be
buried in some government doc. Please Help? Thanks Anne
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His
. Consistently good results.
The system is user friendly and the staining protocols are easy to tweak and
customize.
Highly recommended.
Regards
Anne
Sent from my iPhone
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Dear Lester Raff MD
Yes some of us do visit the Histonet over weekends.
Your posts are so annoying and self-serving.
Please go away!
Thanks
Annieinarabia
Sent from my iPhone
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Hi all
Anyone successfully using IDH1? Have been asked to source but am having a hard
time finding a supplier. Anyone?
Ta
Anne
Sent from my iPhone
> On Dec 7, 2015, at 10:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
>
> Send Histonet mailing list submissions to
>
Greetings esteemed Histonetters
We use commercially prepared NBF in pre-filled containers for collection and
submission of all tissue.
We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on
the gross station and in all our tissue processors.
We also strictly adhere t
Greetings esteemed Histonetters
We use commercially prepared NBF in pre-filled containers for collection and
submission of all tissue.
We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on
the gross station and in all our tissue processors.
We also strictly adhere t
There is a grandfather clause if you were a histotech by april 24.1995. I
asked ClIA recently when they came to inspect me, as I fell into this category
but didn't know if you had to have the licence by then or just have been
performing high complexity test at that time (my licence was later th
ry its going to be a lot of trial and error.
Maybe you can call the previous tech on how they did it there. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rachel M
Gonzalez
Sent: Monday, April
I always used to rinse the containers I used with alcohol and let them dry
before doing the stain. Some people use an acid alcohol rinse. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff
just a paint scraper at your local hardware store. Some people attach it to a
broom handle so they don't have bend down. They may even have a long one.
Anne
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwester
a bit longer to embed
cause the bottom takes longer to warm up so it's a trade off for time. Fun
experiment though! Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha
M.
Sent:
I mark shaves and punches with .5% Toludine blue it won't color the skin but
will color the rest. And best of all it doesn't show up in any staining its
purely for identifying your tissue for embedding purposes. Anne
-Original Message-
From: histonet-boun...@lists.utsouthw
We do them successfully all the time. Without pretreatment.
AnnieinArabia
Sent from my iPhone
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My first job was at a lab that did both human and animal (till a case got mixed
up) the only difference I found was animals were fattier and hairier. Same
microtome. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
Hello there you awesome group of histo gurus...
We have been running the new improved Artisan Gram kits.
They say to use the standard on board staining protocol. This gives us no gram+
orgs.
We reset the CV to its max. Still no blue.
We used the same new kit reagents manually with the same instr
each time. Time
consuming yes. But I find freezing and let sitting I don't fight with it as
much when cutting. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of michele carr
Sent: Tuesday, Septemb
Contact your local labs and Hospitals, you could just use some already
embedded and cut tissue that is about to be disposed of as long as there
is no patient info on the blocks it shouldn't be a problem. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwester
Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
I just got back from the Mohs conference. The Biocare people have the stain
and told me they would come out to help work it up. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bethany
Sent: Thursday
We got some plexiglass dividers at the "Container Store" if you have one
near or check online. Can't remember what they were called but they had
different sized square compartments that fit various venders antibodies.
Anne
-Original Message-
From
Biocare has a herpes I&II mix if that's what you're looking for. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Pathology-Histology Sr. Supervisor
Sent: Monday, April 28, 2014 5:27 A
spital in Spokane Wa Has the complete system so
you could give them a call. 509-474-4108 Thanks Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Wednesday, February 12, 2014 10
Don't forget to make sure you have a spill kit and its labeled well with
easy access, Inspectors will ask employees if they know where it and
your fire extinguisher and alarm are located. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2
years with Clia, like you do CAP specifically Temperature and
maintenance charts. Thanks Anne
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, it has a lot of papers
written on it that might help. Anne
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Wednesday, December 04, 2013 12:19 PM
To: histonet@lists.utsouthwestern.e
If anyone wants to cut down on cutting injuries. Teach the new techs to
use two forceps instead of your fingers. I'm too old to change but I
know a tech who cuts this way and has never had an injury. It works
really well on automated microtomes. Anne
-Original Message-
From: his
I
Wellman Center for Photomedicine
Massachusetts General Hospital
50 Blossom Street, EDW 214
Boston, MA 02114
617-726-6983 (Lab)
726-1206 (fax)
-Original Message-
From: Britton, Josette C [mailto:jcbrit...@cheshire-med.com]
Sent: Tuesday, October 01, 2013 7:41 AM
To: Scanlan, Megan Anne
S
Hi all,
Does anyone have experience cutting human lymph nodes from a cadaver in
paraffin blocks? I am having quite a lot of trouble getting good sections,
even using a brand new blade the sections end up with multiple blade marks
streaking through the tissue and paraffin.
I've contacted the
Can anyone tell me how many gallons of Flammable liquid can be stored in
a space per CLIA regulations. There website is useless. Thanks Anne
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My goodness ignorance abounds...Rene J is one of the most respected voices on
this forum.
And 'she' is actually a 'he' you dimwits.
I would take his advice above almost anyone on this forum.
You youngsters should try to emulate rather than denigrate.
Rene you rock!!!
Annieinarabia
Sent from
Choose the FILM coverslipper.
Sakura and DAKO over Leica anytime.
Annieinarabia
Sent from my iPhone
On 12 Sep 2013, at 9:02 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscrib
67-6600 -Office
(724) 494-6166 -Cell
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tarantelli,
Rebecca Anne
Sent: Friday, August 02, 2013 10:30 AM
To: Sebree Linda A; histonet@lists.utsouthwestern.edu
Sub
-Original Message-
From: Sebree Linda A [mailto:lseb...@uwhealth.org]
Sent: Thursday, August 01, 2013 3:50 PM
To: Tarantelli, Rebecca Anne; histonet@lists.utsouthwestern.edu
Subject: RE: immunohistochemistry help please
OK, so I would be very suspect of your xylene. Has anything changed, i.e
...@gmx.at]
Sent: Friday, August 02, 2013 7:40 AM
To: Tarantelli, Rebecca Anne
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] immunohistochemistry help please
Are all slides from the same tissue sample with the same pretreatment?
I can't believe, that you don't have any hematoxyli
ge-
From: Sebree Linda A [mailto:lseb...@uwhealth.org]
Sent: Thursday, August 01, 2013 3:36 PM
To: Tarantelli, Rebecca Anne; histonet@lists.utsouthwestern.edu
Subject: RE: immunohistochemistry help please
To test your deparaffinization Rebecca, I'd try staining a slide with something
Hi all,
I am new to this forum, but a friend recommended I send my issue out to you for
help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC)
using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked
perfectly. Three days later, after 13 beautiful stai
Annie in Africa aka ArabianAnnie is back and wishes you all a very Merry
Christmas and festive New Year - Happy Holidays
--
Anne van Binsbergen
Abu Dhabi
UAE
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thanks
AnnieinArabia
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Abu Dhabi
UAE
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take the poor animal to a vet - if it is a BCC or an SCC (which are both
CANCER by the way..and it can spread) then it needs proper treatment
would you treat your child like this - I hope not
and just by the waythis is not a pharmacy listserver
On 16 April 2010 20:04, James Ball wrote:
>
t; disclosure, copying, or distribution of this message, or the taking of any
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t-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van
> Binsbergen
> Sent: Wednesday, January 13, 2010 1:16 AM
> To: Denise Van Eaton
> Cc: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Sakura iDent
>
> Hi De
me that the print does not wipe off with xylene -
which will be hard to do seeing that i wiped it off myself and saw with my
own eyes that xylene removes the ink!!
Sakura - if you are reading this - i am not a happy camper
Annie
2010/1/12 Denise Van Eaton
> Hi Anne,
>
> We have been
et
>
>
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; - 80% EtOH - 1-2min
> > - Eosin - 2 min
> > - 95% EtOH I - 10sec
> > - 95% EtOH II - 10sec
> > - 100% EtOH I - 10sec
> > - 100% EtOH II - 10sec
> > - Xylene I - 2min
> > - Xylene II - 2min
> >
> > 7. Coverslip and let dry overnight
> >
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Tissue-Tek xylene substitute - my xylene is made by Fisher
I would love to know if anyone else has had similar issues
Yes, Rene, this is the diehard number 1 Sakura fan having issues with a
Sakura product!!!
hopefully yours (pun intended)
AbuDhabiAnnie
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UAE
list
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stain didn't work. I'm interested in
either paraffin blocks
or a company that uses Plus slides. Thanks for your help.
Marie Anne Morin HT. ASCP
Path Tech Specialist
Histology Lab
Rhode Island Hospital
Office 444-7196
Pager 350-7384
Fa
is 1-800-635-4477.
Have A Good One!!
Marie Anne Morin H.T. ASCP
Path Tech Specialist
Histology Lab
Rhode Island Hospital
Office 444-7196
Pager 350-7384
Fax 444-8514
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on
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U
be frezzing artefact
I will pass the message to them and then I will run and hide!! LOL ;D
thanks
greetings from the desert
Anne
2009/10/7 Tim Wheelock
> Hi:
>
> There is one thing you might try to make frozen brain sections more than
> acceptable.
> You can freeze the tissue in
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