As an instructor for histology, I would completely agree with Renee. If you
plan to become certified you should take the ASCP exam for HT/HTL(depending on
your degree). States certifications differ from real educational requirements
to nothing more than a license to perform your duties. You may
Sorry I don't not have more info on the DAPI user. Unfortunately...or rather
fortunatelyI have never personally had this problem. This is a corporate
user, we have done some HE's for, on FS with no issues. This is just not a
problem I have ever had. I will pass these on to him; they are
Someone sent me a question regarding DAPI staining ( I generally use Hoechst
)...but anyway, they are having smearing of nuclear contents. I would guess
that is from rupturing of the nuclear membraneover fixation? Over air
drying? They are fixing 10 mins in cold acetone and 10 mins to air
Histonetters,
Anyone out there still doing ATPase's on muscles these days? Where are
you ordering your sodium barbital from? We have always used Spectrum.
They no longer carry it. If we cannot find another vendor, is there an
alternate protocol someone might share with us? Thanks guys!
Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
years (on my Perk and Elmer)...Know there must be some great new
reagents and kits out there...opinions? Send to cbar...@nemours.org. Thx
CB
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HistonettersWe have received some dextran beads (coated with cells)
that are snap frozen...for cutting.
We have done these beads before in histogel/paraffin...but are curious
as to how best to handle them snap frozen. We have a nice bead with no
cells on our first to snap frozen sample
Histonetters: Looking for Annexin V Ab porcine reactiveanybody out
there used Annexin V on piggies for histology? What is the scoop? I'd
rather not re-invent the wheel. I am sure, someone has done ths.CB
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I am actively seeking an Immunohistochemistry Vendor to display and
distribute information on their products at a INBRE CORE Facilities Open
House event, Friday April 22nd Christiana Care Hopital - Ammon Ctr. -
Wilmington, Delaware. Event: Each Core is requesting a supporting Vendor
to display
Satelite cells indicate regeneration.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Saturday, April 02, 2011 1:01 PM
To: histonet@lists.utsouthwestern.edu
Histonetters...It has been a longtime concern of mine that the only
company offering TMA instrumentation was Beecher Instruments.and the
wait list for those, was totally unacceptable. It came to my attention
today, from a reliable source at a university that Beecher has gone
out of
HistonettersI am looking for the person who wrote History of
Histology. I believe the author's name was either Mitchell or Michell?
I found it on the web once, but have lost my copy? Anyone out there
remember this article? CB
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Hello Histonetters: We presently have a position available for a HT/HTL
(certified, or eligible) in our clinical muscle enzyme histochemistry
lab (within the Department of Biomedical Research). We request 3 yrs
experience minimum in EHC... and skills for manual IF, as well. If you
meet these
Hello Histonetters: We presently have a position available for a HT/HTL
(certified, or eligible) in our clinical muscle enzyme histochemistry
lab (within the Department of Biomedical Research). We request 3 yrs
experience minimum in EHC... and skills for manual IF, as well. If you
meet these
YOU ARE INVITED!!!
For those of you from Region II, who will be attending the NSH S/C in
Seattle Sept 24 -30th. I would like to invite you to a special meeting
for Virginia, W. Virginia, Washington, D.C.members and any others
interested, from Region II...on re-establishing the By-laws for the
HistonettersWhat are your thoughts:
We are getting ready to purchase a labeling system for our Core
Facility. We have limited $'s and have demo'ed three systems. We have
many different projects,with varying numbers of samples, blocks and
slides. We would like to cover the widest amount of
Nemours is in-search of an HT or HTL certified, or eligible - tech for
Histotechnology Core Facility, Wilmington Delaware. Position 50%
clinical/50% research. Background in enzyme histochemistry, IF and IHC
required. Molecular histology, LMD or Imaging a plus. contact:
cbar...@nemours.org
We use a modified Gomori's one stepwith excellent results on frozen
muscle. You can find the protocol in Muscle Pathology and
Histochemistry, Harvey Sarnat. It is an excellent stain...and if you
have a limited amount of tissue, it gives you the most information on a
single slide(and if the
Histonetters:Here are the sessions open for your interest - Region II
Symposium:
* Thursday June 10: Region II Registration opens for registration
at 12:30: Session 1: Trouble shooting Histology Stains, Session 2
Manager's Forum, Friday June 11, AM -Session 1: VIR Seminars, Session 2:
Region II symposium: Download a meeting brochure from the NSH website
under state meetings at www.nsh.org/content/region-ii-meeting.
June 10,11 and 12- Atlantic City NJ
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Histonetters...
Good news for you..if you are one of those people who are always late or
procrastinating! Pennsylvania's late reminder ...is your benefit!
Flyers regarding the Region II Symposium in NJ, were just sent to
eastern PA facilitites last week (due to the temporary inactive state
Histonetters- Region II has extended the mail-in registration deadline,
until Friday May 28th...just a few days away! We know some people are
still awaiting travel approvals and we are doing our best to accomodate
everyone who wants to attend and take advantage of the early
registration. I went
Histonetter's: June 10-12, 2010 Clarion Hotel Convention Center ...
Our Featured Speaker this year is Peggy Wenk. She is the Director of the
HT/HTL Program at William Beaumont Hospital in Royal Oak, MI. In 2007
Peggy received the NSH Histotechnologist of the Year Award, is currently
the
Region II Managers on the Histonet:
Opportunity to attend the Managers Forum - Region II Meeting/Atlantic
City West, NJ.
Date: June 10
Time: 1:30-5:00PM
Session Title: Manager's Forum: Roundtable Discussion for Anatomic
Pathology Managers and Core Labs
Panel of Experts:
Carol Barone
I never throw a book away! cbar...@nemours.org
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Saturday, May 01, 2010 1:01 PM
To:
Histonetters familiar with CAP and CLIA compliance issue...I am
requesting a clarification:
Will the new CAP guidelines related to processing and grossing
affect CLIA regulations? I understand they will certainly affect CAP
labsbut do they impact CLIA labs as well? Is there any
grandfathering
Hello histonetter's...I come one again to the experts in the field.
I have been give two HE slides from an autopsy case (have no idea how
old)...and have been asked to retieve this tissue into an eppy with
lysis buffer for DNA. Does anyone out there have an existing method for
doing this? I
Histonetters- Back to these DRGs again:
We are looking for a better way to embedd DRGs from neo-natal mice, for
cryotomy.. We normally...using a dissecting scope, remove the DRG from
the eppendorph tube with a small spatula from ...touch the drg to some
OCT (colored) and then touch the OCT
Histonetter's I only received one reply on my question regarding what
everyone is doing for proficiency testing of enzyme HC staining for
muscle...espeically for CLIA.compliance, now that I will lose my
indepent reviewer.
My thanks to Jan Minchew for her reply. I am working from that for now,
I am looking for proficiency testing for my staff for enzyme
histochemistry. Presently we have been using a pathologist (not related
to our organization). He is planning on retiring in June. We are a CLIA
lab. I have noted EHC is not a speciality offered by HQIP. What are
other EHC labs doing if
Histonetter's, Members of Region II and beyond.: Save the date for the
2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic
City, N.J Come for a day. Come for the weekend. For more information
about registration and accomodations contact Michele French:
609-818-3278 /
Histonetter's, Region II up-date: Save the date for the
2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic
City, N.J ...Come for a day... or come for the weekend.
Excellent Speakers, new workshops, vendor fair and of course...there is
always the beach.
..(for studying, of
DRG's = Dorsal Root Ganglion... To clarifyhealthcare is safe from these.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Saturday, February 20, 2010
Histonetter's...we received a boat-load of mouse DRGs that had been
prepared in histogel and are cutting...well..not so good.
We normally do DRGs from FS and get beautiful results.
We have used histogel before with other small sample and have never had
issues... not sure if it is the Histogel
Thanks you to the attendees of last Saturdays, very successful VA
mini-LEARN and EARN in VA Beach. For those who attended, and wish color
copies of the handouts... send a request for the site posting:
cbar...@nemours.org
Congratulations to Virginia Society ... for your efforts in
re-activating
Virginia Histo-nettersI am trying to re-locate the person from
VA Tech.edu...who e-mailed me and was interested in being part of the
new VA Histology Society?... or anyone at VA Tech...
No matter where you are, anyone, ...if you would be interested in
paticipating. contact me at:
Thank you.
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The most in-expensive way to earn your needed CEU'sdidn't make it to
Birmingham? Come to Virginia Beach, Virginia This week-end! A day to
learn and earn CEU's, for as little as $35.00. Open registration at the
door.Virgina and surounding states ...reminder of the fall
Histonetter's in the VA, MD and DC area...reminder of the fall
mini-LEARN and EARN to be held this Saturday, November 7th 2009, at the
Virginia Beach Higher Education Center. Six (6) CEU's available, Two
Speakers: Carol Barone HT(ASCP) BA - Frozen Sections: Materials ,
Methods and Mastery (3
Histo-netters? Looking for the best method to process some mouse eyes in
paraffin. Let me know about the fix, too...(.if it is something exotic?)
I have a project coming my wayand I am sure there is someone out
there who has done this a million times...and I don't need to re-invent
the wheel
I agree 100%. Beecher is the worst vendor I have ever dealt with...and
unfortunately for Tissue array equipmentyour choices are pretty much
Beecher, Beeecher or Beecher..Chemicon had one, at a time...but, Beecher bought
it from them. Isn't there some laws about monopolies? They seem to have
Have also been doing them since 1982know what you mean about the artifact.
We store in Bell-Art containers (availavle thru Fisher)..We have found no
others to have the ability to storewithout cracking for so long.
We do place about 100 ul of water in the storage container...and place it in
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 68,
Containers: Fisher # NC9283918 $32.90/6.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, July 29, 2009 1:01 PM
To:
Histonetter's:
#1- What are your thoughts on the many new sites for Histology
assistantsthey come very close to sounding like Histotechnicianswhich
they are not. I am not 100% on allowing another category for assistants...but
, would like a true differentiation between assistant,
VirginiaHistonetter' s: Spread the word Walk-ins will be welcome!
Learn and earn 5 CEU's in a single day at Old Dominion Higher Education
Center, Virginia Beach, VA this Saturday , May 16th. Pre-registrations has
closed...but, same-day registrations will be held on site for
Hello-o-o-o Histonetter' s. I have had several calls and e-mails from
those wishing to attend this Saturday's Seminar's at Old Dominion Higher
Education Center, Virginia Beach but, can't get their checks in time to get
the $10 registration fee waived. If you have an approval..please
What? One day/2 seminars/ 5 CEU's in relevant area of IHC. When? May 16,
2009, Where? Old Domininion University Higher Education Center. Virginia Beach
VA.
Who? Hosted by the Regional states of DE, MD, NJ and PA...and supported by
Biocare. For further information and registration:
Histonetter's: Looking to get those needed CEU's or just want to learn
something new? The states of PA, MD, NJ and DE are hosting a regional one day
event on Saturday,May 16th, 2009 ...for Virginians and state and NSH members,
at the Old Dominion University Higher Education Center, in Virginia
Histo-netter's:
Earn 5 CEU's in one Saturday at the beach!. Virginia Beach, Virgina. Hosted by
the states of DE, MD, NJ and PA...this is not a Region II meeting, rather a
meeting hosted by sister states to help the Virginia Society back up to it's
feet...while you earn CEU's in one dayand
Is this OK for the Histonet:
ATTENTION!:VA, PA, MD, NJ, DE, W. VA, and DC
Earn CEU's at a state hosted regional meeting to be held in Virginia Beach,
VA. Saturday, May 16th. Hosted by NJ,DE,MD and PA...One day event/ earn up to
5 CEU's. Hosted by PA, MD, DE, and NJ. Sponsored by Biocare. For
Histonet: I have a couple questions I would like to throw out to the experts:
1. Do you think higher yields of DNA /RNA in LMD are related to protocol,
method differences from one instrument to another ( i.e. catapult verses,,
gravity drop, verses,
arcturus melt system...or more related to
, FREE MEDICAL BENEFITS!
(Alyssa Peterson)
10. Frozen section (Martin, Gary)
11. RE: Frozen section (McNabola, Angela)
12. Re: Current books for Histotechnology (Geoff McAuliffe)
13. RE: Frozen section (Weems, Joyce)
14. RNA and DNA yields from Laser Microdissection (Barone, Carol
Hitech'er's: Is there a fluorescent system (chemluninescent or bead system) for
quantitatative reading of fluorescently prepared tissue sections? reader/
Scannerand used with what kind of detection? Where do I start?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hello colleagues of the tissue world!
I am working on developing (revising, actually) up-dated standards for CLIA
proficiency testing in my lab. We are only doing Histochemistry, Immuno
fluorescence for dystrophin and dystrophin assoicate proteins and
Immunofluorescence for both kidney and
Histonetters: I have been doing muscle enzyme histochemistry since 1984 and
have yet to find anything that works as well, with consistant results, as
isopentane. Using it safely under a hood and kept in the explosion proof frig
(no exposed electric parts that could arc) can be accomplish. I am
-Original Message-
From: Barone, Carol
Sent: Thursday, November 20, 2008 3:17 PM
To: '[EMAIL PROTECTED]'
Subject: help
Importance: High
Histo friends...I am working on a five year strategic plan for a Research
Histotechnology Core Lab with very diverse
???
Any recommendations for either of these:
1.Processing and cutting of longitudnal sections of PVFD fibers...sometimes
called hollow fibers. We are having issues keeping the sections on the
slides, no matter what slides we use/ or coat?
2.Di I staining...who has a histology protocolfor
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