Diff Quick appears to be a modified Field's stain. The blue dye in the
methanol is one of the modifications. Field's stain gives much the same
staining, so using plain methanol should be of no concern. The simplest
way to find out is surely to try it and see.
http://stainsfile.info/stain/micro
Hi,
Try the method given in StainsFile at:
http://stainsfile.info/stain/metallic/jones.htm
Bryan Llewellyn
Hood, Jordan via Histonet wrote:
Hello,
I'm new to histology (and new to histonet), and I work in a small histology lab
specializing in animal tissues that receives requests/submissions
Use surface decalcification:
Surface the block, then remove from the microtome. Do not use the
microtome for another block, so as to avoid adjusting the position of
the block face to the knife.
Place the block face down in 4% nitric acid for 2 hours or longer.
Rinse the block and cool down
StainsFile is still available at http://stainsfile.info
In the downloads section there is a scanned, corrected and reformatted
copy of the Microtomist's Vade Mecum, 7th edition. This text covers
other areas as well as medical and is in the public domain in the US and
Canada, and likely elsewhe
We used to have a form with two sheets. The front sheet was a "magic
carbon" so the patient information was automatically transferred. During
booking in of the specimen, we separated the sheets. The back sheet
accompanied the specimen for the gross description and the top sheet
went directly to
I have sectioned both standing and sitting, and much prefer sitting.
However, due to back problems I found it necessary to have a stand built
for the microtome so that when I was sctioning I reached slightly
upwards rather than crouched over. This all but eliminated the strain on
my back. The s
I suspect disposal might vary depending on the State. I live in British
Columbia and we had permission from out city (Prince George) to use a
dribble tank with lots of water and flush them into the local river (The
Fraser), but I opted to collect all the toxic chemicals and ship them
periodical
Hi,
I presume you are talking about light microscopy. Glutaraldehyde is
often used for electron microscopy. If that is the case, ignore this.
Glutataraldehyde fixes similarly to formalin, so the morphology should
not be much different. However, it leaves the tissue with free aldehyde
groups a
If you can't find suitable pin out boards, they can be made easily
enough. Obtain some suitably sized plastic containers with lids and fill
them an inch deep with used, filtered, paraffin wax that would otherwise
be discarded. After using, just remelt, filter if needed, then harden again.
Brya
Cold acetone, and cold ethanol, were used to fix tissues because they
left enzymes unaffected and still demonstrable. This was in the early
days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and
applied,3rd edition, volume 1, page 85 discusses it. I could send a scan
if you want
All of the Picro-Mallory variants are trichrome stains. An explanation
of how they work is here:
http://stainsfile.info/StainsFile/theory/tri_gen.htm
http://stainsfile.info/StainsFile/stain/fibrin/fibrin.htm
Follow the links as well for added information.
Bryan Llewellyn
Пешков Максим via H
1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works,
as does 1% safranin. Both are stable, although should be filtered from
time to time. Stain 1 minute, water rinse, ethanol, xylene and mount
should work. I had one pathologist who preferred a very light
progressive H&E. She
Go to http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm
Bryan Llewellyn
Tyrone Genade via Histonet wrote:
Hello,
Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections?
Does anyone have a protocol?
I want to examine hematopoietic tissue of fish, i.e. the head
Sorry!
That should be TSP - trisodium phosphate - not TCP, which might make it
worse.
Bryan
Bryan Llewellyn wrote:
This used to be a common problem years ago. It is due to crud buildup on
the metal. Boil them with TCP for half an hour, then thoroughly wash
them in cold water. Coat them with
This used to be a common problem years ago. It is due to crud buildup on
the metal. Boil them with TCP for half an hour, then thoroughly wash
them in cold water. Coat them with a VERY light smear of glycerol before
you use them, preferably each time. That should help.
Bryan Llewellyn.
Diane S
Please read the article at
:http://stainsfile.info/StainsFile/stain/elastic/elastic.htm
There are numerous alternatives discussed there. I particularly like the
Humberstone variant of iron resorcin fuchsin.
Bryan Llewellyn
Nirmala Srishan via Histonet wrote:
Histonetters,
Is there someone
You can use either the sodium or potassium salt. Both can also be used
to make Schiff's reagent as well. In fact, many histotechs leave out the
sulphite rinse step and simply rinse off in water and wash well with tap
water. It seems to work just as well.
Bryan Llewellyn
Angela Lamberth via H
I played around with it many years ago and found no difference except
for the bluer tone. Simply substitute it for eosin Y ws gram for gram,
and use the same technique.
Bryan Llewellyn
Bob Richmond via Histonet wrote:
Gudrun Lang (in Austria) asks:
I wonder how widespread the usage of eos
Aniline oil is more commonly known just as aniline. Sigma list it for
sale at:
http://www.sigmaaldrich.com/catalog/search?term=aniline&interface=All&N=0&mode=match%20partialmax&lang=en®ion=CA&focus=product
Bryan Llewellyn
Susan Bachus via Histonet wrote:
I'm trying to locate aniline oil for
I think it is rather unfair to accuse Dr. Raff of harassment or misusing
this forum. I recall that when he first posted references to his blog he
asked for the members' permission to do so, and he was given that
permission. Rather than abusing the privilege of posting to Histonet, I
think he sh
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