[Histonet] Kappa/Lambda bone marrow

Does anyone out there have a Kappa and Lambda protocol for ISH on the Ventana BenchMark Ultra? Was also wondering what type of decal should be used for ISH? Thanks! Brandy Burnett BS,HTL(ASCP) QIHC bburn...@capecodhealth.org ~~

Re: [Histonet] Ventana LCS (Liquid Cover Slip)/ non-specific staining

Expert physicians. Quality hospitals. Superior care. -Original Message- From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] Sent: Thursday, July 02, 2015 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues

[Histonet] Ventana LCS (Liquid Cover Slip)

Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Hi

[Histonet] RE: Nuclear "Artifact"

We are having similar issues with our tissue. Any troubleshooting insight would be greatly appreciated! Thanks! From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sue [suetp...@comcast.net] Sent: Tues

[Histonet] RE: IHC Billing Question

We recently added HER2 IHC testing in our lab which we are required to use a negative reagent control For each case. Is there a cpt code for negative reagent control reimbursement? Any information on this Would be much appreciated! Thanks Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathol

[Histonet] tissue fallin off CAP slides

Has anyone out there had any issues with tissue falling off of the CAP slides for ER/PgR surveys? I recently found out that they are embedding their cores in a paraffin with a much higher melting point than ours. Could the incomplete removal of paraffin cause the tissue to fall off the slides

RE: [Histonet] p16 Antibody

We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists

[Histonet] HER2 IHC controls

Are any of you making your own HER2 IHC control slides from patient tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed.

[Histonet] HER2 IHC score averages

Would anyone happen to know the overall scoring averages for HER2 IHC for each score (0, 1+, 2+, 3+)? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity

[Histonet] HER2 scoring exercises for breast cases

Does anyone know of a good website for practicing HER2 scoring on breast tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addre

RE: [Histonet] Re: Papillary Vs Reticular Dermis.

You can use Smooth Muscle Actin, Calponin, Myosin and CD34 to stain the vessels. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cesar Francisco Romero [chesar...@hotmail.com] Sent: Friday, Septembe

[Histonet] RE: Frozen sections for Immunoflouresence

Are you using pre-cut control slides? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [vickroy@mhsil.com] Sent: Tuesday, September 02, 2014 10:51 AM To: histonet@lists.utsouthwester

RE: [Histonet] IHC QC forms:

Cape Cod Hospital From: Sebree Linda A [lseb...@uwhealth.org] Sent: Thursday, August 21, 2014 5:19 PM To: Burnett, Brandy; 'Morken, Timothy'; 'Jb'; 'Histonet' Subject: RE: [Histonet] IHC QC forms: Brandy, We are completely pape

RE: [Histonet] IHC QC forms:

Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? T

[Histonet] tissue falling off slides

I forgot to mention that we are using a 3 in one HIER for target retrieval, instead of running down (xylene-ETOH). Could this also contribute to tissue falling off? ~~ This email and any files transmitted with it are confiden

[Histonet] RE: Tissue falling off

atory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the pa

[Histonet] tissue falling off slides

Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated

RE: [Histonet] RE: p16

Do you know what the dilution is from Biocare, and how many tests per vial? From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Wednesday, August 06, 2014 5:02 PM To: 'Ronda Mire'; Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu;

[Histonet] RE: p16

I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. Thanks! Brandy Burnett, HTL (ASCP) Cape Cod Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf

[Histonet] Calponin

Hi All, I was wondering what stain protocols are being used for Calponin to minimize cross-reactivity with myofibroblasts? We are using a Dako concentrate antibody. I recently re-optimized the stain using a 1:100 dilution and changed the pre-treatment from low pH HIER, to Protease only. This

[Histonet] ER+/PR- in elderly patients

I was wondering if anyone is seeing a trend in ER+/PR- tumors more frequent in older patients? If so, do you have any information regarding this? Thanks~ Brandy Burnett, HTL ~~ This email and any files transmitted with it ar

[Histonet] Cytokeratin AE1/AE3

Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this is