Re: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining?

Hi Cheryl, I have heard of people using cucumber and processing it, but I have not actually tried it out myself! One of my R&D friends recommended it to me when trying to DIY some tumor microarrays. You might be able to try it out and see if it stains well - I have never actually seen it in person

[Histonet] Leica Slide Printer

Hi all, I am looking to see if anyone here uses the Leica HistoCore PERMA S Slide printer/s in their lab? The integration with the Bond III IHC stainer makes them highly appealing to my team since it will allow us to error proof the IHC staining process. However, my finance/business folks are worr

[Histonet] Dako PD-L1 questions

Hi all, I am looking to connect with people that stain PD-L1s on either of the Dako Autostainers. My company has been asked by a research/pharma partner if we would switch our IHC staining system to this brand. We currently use the Leica Bond IIIs. What I am curious about is how long a run takes,

Re: [Histonet] Human Tissue Blocks

Hi Chakib, I am not sure what kind of budget you have for control tissues, I work in the private sector unaffiliated with any one medical center so we routinely have to buy tissue for control blocks and validations of new antibodies/assays. We use two different suppliers - RS Diagnostics

Re: [Histonet] Help to interview new employees for the first time

Hi Blanca, I think practical skills tests are a great idea. BUT I also think you need to make sure that having someone that isn't an employee doing tasks in the lab is a responsibility your lab would willingly take on if something were to happen. If the candidate cut themselves and was litigious t

[Histonet] Tissue scrolling

Hello Histonetters, I am curious if any of you perform scrolling of paraffin embedded tissue for molecular studies? If so, what precautions do you take to minimize contamination? I am asking specifically about at the cutting station as that is my area of expertise. But we are looking to pilot this

Re: [Histonet] Antibodies

Hi Laura, What stainer did you previously have? I might be interested if it is from either the Roche or Leica platforms as we have both currently at my lab. Also what antibodies? Thank you, On Thu, Aug 22, 2019 at 8:22 AM Jones, Laura P. via Histonet < histonet@lists.utsouthwestern.edu> wrote:

Re: [Histonet] Use of Freeze Spray in a Cryostat

Hi Jennifer - It was my understanding that the recall was particularly related to the flammability of the freeze spray. If you can find a spray with out a flammability hazard you can keep using the spray. I don't have a lot of frozen sections in my current job. But in my last job, and my experienc

Re: [Histonet] What would your advice be for a New Grad? I speak

I agree with what Toysha said, and also don't take your more experienced techs for granted! There is great value in techs that have been at this for a long time. They may not seem tech savvy, or fast to you now, but some of them have been at this longer than us younger techs have been alive! Some o

[Histonet] RUO antibody

Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showi

[Histonet] LDT question

Hi All, I have a validation question - we are looking into buying an RUO rated IHC stainer for a future multiplexing project. Roche's Discovery Ultra to be specific. I have a pathologist that is convinced that we can run our clinical antibodies on it as an LDT and still bill for the work. The mat

[Histonet] Molecular Testing

Hi All, When cutting for NGS testing what additional measures do you take to minimize contamination? We are a pretty clean outfit here, but still, have some trace sample carryover occurring in cases we cut in-house. We already use a fresh blade with every case, use tools like forceps instead of fi

[Histonet] Whole Slide Imaging

Hi All, I am curious if any of you have used a whole slide imager, and if so how did you structure your validation plan? Our lab does not make primary Dx, we review samples for tumor content to use for NGS testing. We record images so we can keep a record of the tissue once we send the slides back

[Histonet] Suggested Requirements for new uncertified HTs

Hi All, I work at a Start-Up and I was our first hire. I have been practicing since 2011 when I got my certification. However, we are looking to expand, what would you suggest our baseline technical competencies be for a position that focuses exclusively on cutting and IHC. In an interview it is h

[Histonet] HPV DNA Probe question

I am curious what people are using for HPV probes. We recently stumbled upon the fact that our current probe choice is RUO - to avoid a sticky validation and LDT process I would like to replace that with an ASR or IVD ideally. I cannot seem to find one, however. Additionally, our Pathologists pref