, are the biopsy and the mouse tissue fixed and processed identically?
Geoff
Edwards, Chris wrote:
Hello. Our lab has had highly variable results using toluidine blue to
stain 1.5 micron thick cross sections of plastic embedded nerve tissue
from both mouse and human nerve biopsy. It seems like
,
ie. the brain is too cold. We always wait 30 minutes or longer.
Store sections at -80, -20 is not sufficient.
Sharpen the knife and check the angle.
Geoff
Guillermo Palchik wrote:
Dear Histoneters,
I am looking for help regarding flash freezing of rat pup brains
(Postnatal day 8). We need
as important. I'm
sure there is a professional level camera store (not Best Buy) in Dallas
that can help you make a decision.
Geoff
Reuel Cornelia wrote:
Hello histonetters,
I am looking for a good digital camera for gross photography. Any
recommendations that works with your lab
this purpose.
Geoff
TF wrote:
Hi, i dont want to use PFA for the brain fixation (rat).
Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I
do post-fixation at room temperature for 24 hours.
I also tried saline perfusion, then I directly put the whole brain into 70%
alcohol
in
alcohol will wash all of the stain out.
5. Clear in xylene as usual. Use fresh xylene, not solutions containing
carry-over alcohol from other procedures.
The second advantage of acetone dehydration is that metachromasia of
cartilage, mast cells of some species, etc. is preserved.
Geoff
with either.
Geoff
Stella Mireles wrote:
All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any
other company that I should consider. I plan to purchase an Ultra and a
Histo knife.
Thank You
Stella
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Hi Sara:
I, and it seems others, disagree with the idea that alcoholic formalin
is the best fixative for CNS. Buffered formalin is the fix of choice for
animal and human CNS.
Geoff
Breeden, Sara wrote:
Yes, I'm back. I need the expertise of this group.
I am trying to convince my
.
Geoff
u know wrote:
Dear Listers,
If you ever opened any strange attachments from this list, you should really
consider scanning your Hard Disk for spyware. There is a great free anti-spyware
program we use, which you can get at http://tinyurl.com/ynupj4 that is a
virus-proof link
Scientific, I have been a loyal customer since grad
school. Maybe someone had a bad day or was interrupted during the mixing.
Mixing buffered formalin in you lab is so easy and you retain control of the
most important step in histology.
Geoff
Randolph-Habecker, Julie wrote:
Folks,
I need some
It is getting difficult to remember when an item that actually dealt
with a histotechnology issue was posted.
This is how worthwhile discussion groups fail, endless prattle followed
by endless prattle about the endless prattle.
Geoff
--
**
Geoff
Looked like a scam to me so I hit Delete immediately.
No real name, no real address = scam
Geoff
Mark Ray wrote:
What I got was a Rick Roll music video on Youtube. If aa aa is a
spammer, this was a slick way to attract our attention. Anybody else?
aa aa wrote:
Dear Histotech Experts!
I
barrier means. The bbb
is a very complex entity with both physical and metabolic components.I
suspect the person who told you this is not well informed.
Geoff
Boma Fubara wrote:
Can someone explain to me the mechanism by which glutaraldehyde would
disrupt the blood brain barrier if the brain had
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Geoff
--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane
A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner
and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's
HISTOLOGY, the
third edition by Ross, Romrell and Kaye is much better and might be cheaper.
Geoff
Smith, Allen wrote:
For an atlas, Ross and Pawlina's
Try a university library, OSU comes to mind. If you are close to a state
univ. that has this in their collection you could go there and copy it.
Otherwise they will probably charge you $1-2 per page to copy it.
Geoff
Suzanne Bruce wrote:
I keep seeing references to this article, but am
Try embedding the spine in the buttocks of the person who embarked on
this project without consulting you first.
Geoff
Elizabeth Earle wrote:
Any ideas about how to get a section from FFPE cactus spine? They have
not been decalcified; seem to have been just dropped into formalin
@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
fine.
Geoff
TF wrote:
Hi all, i amusing Feulgen staining to visualize the apoptotic cells on 20 um
brain frozen sections.
The procedure is 5N HCl room temperature for 1.5 hour, wash in cool 1N HCl,
wash in water, Schiff staining room temperature 1 h, wash throughly in
distilled water
I was taught 4 changes of 50% EtOH, 1-1.5 hours each. The goal is to get
most of the picrates out of the tissue for long-term storage (of blocks)
I don't know if one can ever get all of the yellow color out.
Geoff
Jennifer Anderson wrote:
Hello.
I am looking for information regarding
Hi TF:
Your problem should not be happening.
Sounds like poor perfusion followed by immersing the whole brain (with
the wound deep in the interior) in a small volume of fix at 4 degrees C.
What fixative are you using?
Geoff
TF wrote:
Hi, I just want to fix the damaged brain tissue (2-48
it just does not matter.
Geoff
Pat Flannery wrote:
Please humor me on this if it's obvious (to everyone but me): why do
we use paraformaldehyde (which is so inconvenient to make up) rather
than buffered formalin or just diluted formaldehyde itself?
It seems that around here, some folks
Does not matter. The only error I see people making is spending a lot of
time on the washout step. You will never get all of the blood out so
there is no reason to try. Given a 250 g rat, I would use 25-30 ml of
washout instilled in 15-20 seconds, then switch over to fix.
Geoff
Neil
Or like an occasional chair?
Geoff
Joe Nocito wrote:
is a casual histo tech like a casual end table? What is it the rest
of the time?
Oh, yeah, we not only have a great cents of humor, we're real cut ups to.
JTT
- Original Message - From: Dianne Holmes
[EMAIL PROTECTED
worthy projects to fund and cannot
fund them all.
Geoff
Emily Sours wrote:
Isn't the election over?
Griping will do you no good, spambot!
So, being in grant funded research, I hope our new president will put some
money back into NIH.
(I was hoping that no matter who won, so my political views
Hi Betsy:
Wow, a real histology posting! If it is any comfort I have had the
same problem with mouse heart after Zenker's fix (I wanted nice
intercalated disks). I think the solution is to use NBF.
Geoff
Molinari, Betsy wrote:
Hi,
I processed some mouse hearts that were in Z-Fix for 24
but NOT after glutaraldehyde fixation.
Geoff
TOJO (Torben Seested Johansen) wrote:
Hi,
I do not seem to be able to achieve acceptble morphology of perfused rat liver. I seems
as if some of the cytosol of the hepatocytes are washed away as seen in
toluidine blue stained cryo-sections (see image18.jpg
You can paint stainless steel if you prepare the surface and prime
correctly. Call the help line of Benjamin Moore, Sherman-Williams,
Zinsser or ?? and tell them what you need.
Geoff
DiCarlo, Margaret wrote:
Hello Histonetters,
Working in a Bone Lab, I cut large sections which often
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