Re: [Histonet] A suitable negative control for BAP1

Patrick, We've been studying BAP-1 too and do not have a good answer for a truly negative tissue, except for skeletal muscle, but decided against it. Our current multi control block contains: 1) lung ca, 2) tonsil, 3) colon ca, 4) LN, 5) ovary ca, 6) lung. Our clone specifically stains each

Re: [Histonet] semi-automated/automated TMA instrument to buy?

Hi Adriana, We had a Beecher but it acted stupid, so we got a Pathology Devices semi-automated arrayer. We typically build ~ 100 blocks per year, under a variety of study banners, so it's ideal for

Re: [Histonet] Removing paraffin sections from glass slides

Hi Anna, You could deparaffinize/rehydrate to 10% (or so) bleach. Section(s) comes right off after ~1-60 minutes (or so). Did this by accident (that was a bad day). Deparaffinzation via Rene J Buesa's DWS (http://www.histosearch.com/ADP7HistologyWithoutXylene.pdf) method, or simply

RE: [Histonet] Masson trichrome and H and E

Hi Emily, My $0. 02 by saying it's a challenge for research groups, like ours, to keep quality stains that are not used everyday/week/month. I agree with your intuition. Buy or make new eosin. As for stocking eosin (or hematoxylin), I buy premade by a reputable vendor and always keep an

[Histonet] RE: ASP300S or VIP 6

Hi Jim, Both machines have given us years of service and we (pretty) highly recommend either. However, part of your decision should encompass service. I recommend buying two of the same. Validation was easy (well, sort-of) on either machine. Notes and problems we've encountered with our

[Histonet] RE: Histonet Digest, Vol 127, Issue 9

Hi Ronda Mire, Your best bet is to find someone who discarded an old VIP and has kept their baskets when they purchased new machines, to sell you theirs. Sorry I don't fit this criteria anymore. We have thought about replacement parts too, but have not found baskets that work as well as the

[Histonet] RE: Histonet Digest, Vol 127, Issue 2

Sylvia, I per diem at a lab that has four Microm HM355 S microtomes (previous generation), and they work well; easy to get used to and dependable when serviced properly. Your HM 325 appears to have a similar mechanism that served their needs for years. The Thermo Finesse is also a decent

Re: [Histonet] Cryostat

Tanya and Madeline, Jan Minshew of Leica sent a nicely detailed response to Decontaminating a cryostat in January of 2010. She apparently wrote it to put in a Leica How To manual, although I did not notice it in this form. Everything she states is well documented, although I also

[Histonet] Freezer for tissue storage

Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys

[Histonet] Asbestos microtomy advice?

Hi folks, Odd question (one I never expected until this week): if a researcher was injecting fairly large asbestos loads (~75-100 injections) into research mice, would you use extra precautions while performing microtomy? On thousands of tissue blocks? The injection sites are so numerous, we

RE: [Histonet] Sakura film coverslipper

Marcellyn, We have never owned a film coverslipper because in Hawaii, the only medical center with a film instrument ($) had problems after 5 years. The film pulled off the slides; meaning, they wanted to go back and re-cover tens of thousands of slides, in order to stay compliant.

[Histonet] Histonet

Sophia, Holes like you mentioned, are probably from the polymers or impurities in your embedding wax separating. Gayle Callus mentioned this several times (see histosearch), and recommended Stirring the embedding paraffin every so often (before every use). It could also be your tissue

[Histonet] RE: Histonet Digest, Vol 112, Issue 28

Hi Sophia, Your message is many hours old, and hopefully you figured out something already. Maybe this will help next time. What stains were you going to do with these slides? Our list of Stain removers may not be compatible with your desired stain. 1) 1% Acid (HCL), ~20 minutes, depending

[Histonet] RE: Microtome blades

Laurie, Accuedge can also be found through: VWR (https://us.vwr.com/store/catalog/product.jsp?catalog_number=25608-964) or Electron Microscopy Services http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx I believe we're in a similar situation, as our institution has Favored

[Histonet] BioInventory (Pathology) software recommendations?

Hi all, Can I ask for recommendations for a Tissue block repository software? It needs to incorporate FFPE blocks and their usage in research projects like TMA construction or RNA/DNA testing. We will be most impressed with ease of use and pre-built categories with barcoding. It must be

[Histonet] Tissue Micro Array Devices

Hi Carol Freeman, I work in a TMA lab in Hawaii. We own, and highly recommend, Pathology Devices semi-automated TMArrayer and a Beecher TMA-1. However both are more expensive than the routine pathology lab can easily afford. If you Google TMA arrayer and you will get several

[Histonet] Union positions?

under water). We have federal, state, city/county and Kaiser Permanante, positions that most think of being unionized. Most of us were offered to join, but union tactics and dues were not really what we got into health care for. Hugh Luk, HTL (ASCP) UH Cancer Center- PSR lab manager Honolulu

RE: [Histonet] Manual embedding

Scott, Pacific Southwest Lab Equipment in California has been good to me. Very fair prices compared to new. However, I also must state that two of the four items purchased Used from them no longer work (embedder, cryostat) BECAUSE of the new freon regulations. Be aware that in

[Histonet] How hot is too hot for storing tissue blocks?

Hi folks, We were hoping someone could recommend a temperature range acceptable for storing tissue blocks? Also do you folks use a regular thermometer or a temperature chart recorder (records on graph) to record room temps? We are going to move to a new location and need help with the fine

RE: [Histonet] Unlabeled specimens

Hi Tom, Consider how long it would take to send it back to get a response. Some clients may be far away. If that Non-gyn needs to be processed, we would call them, document the information and contacts, and politely remind them to fill out the forms. Yes, this can be time consuming. In

RE: [Histonet] Diff-Quik stain

How about this from PolySciences Inc. $68. http://www.polysciences.com/Core/Display.aspx?pageId=98categoryId=203productId=2865 Looks compact and transportable. Too small? From: powell...@mercer.edu To: histonet@lists.utsouthwestern.edu Date: Wed, 27 Apr 2011 16:52:55 -0400 Subject:

RE: [Histonet] silver stain on slide-mounted FFPE brain tissues

Dr. Dean, Histotechs are familiar with brain degeneration Silver Stains on FFPE cut sections(slides). Common silver stains include Bielchowsky's method for neuritic plaques and neurofibrillary tangles (most requested by my pathologists for degenerative brain disorders), Bodian's method

RE: [Histonet] Biopsy Run

Hi again Kim, From your previous emails, it seems to be something your tissue processors cannot handle. Review your equipment and consumables. Below, is (almost) everything I think about when I hear Some tissues are staining/processing irregularly: Formalin?

RE: [Histonet] RE: Beecher Instruments

is pricey. However, if you can afford it and make use of it, I recommend it. Sad to hear about Beecher. Have a nice weekend. Hugh Luk Pathology Shared Resources Lab Manager University of Hawaii Cancer Center 1236 Lauhala Street Honolulu, HI 96813 From: hfe...@jhmi.edu

[Histonet] Dr. Tony Manoukian of Maui

Hi histonetters, Some of you may know Dr. Manoukian, then it is my sad duty to inform you of his passing. He worked as a forensic pathologist and was one heck of a great guy. For us in Hawaii, he passed too soon.

RE: [Histonet] Equipment purchases responses

exchange. An example of our 36x24x30 self contained hood is in the link below: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=82010-736inE=1highlight=82010-736 Hope this helps, Hugh Luk, HTL (ASCP) Pathology shared resource lab manager 1236 Lauhala street, Honolulu, HI 96813

[Histonet] Snap freezing using a Dry Shipper?

Hi Histonetters, I need some advice as my group is tasked to procure tissue from surgery sites off-campus. Logistics within those other hospitals do not allow a liquid nitrogen source, and my health and safety folks have asked us to consider using a Dry Shippers or cryoport for snap

RE: [Histonet] Slide Clips for Shandon Linistain GLX

Alan, I use these too, but I'm not sure what is available. I found the web address at: www1.fishersci.com/wps/portal/PRODUCTDETAIL?productId=9097251catalogId=29103pos=25catCode=HC_SCfromCat=yeskeepSessionSearchOutPut=truebrCategoryId=57660hlpi=yfromSearch= is the Fisher Linear stainer (as

RE: [Histonet] I recommend NOT opening that Attachment:

and exclaims, Darn it! Some a$$ has my pen! Respectfully, Hugh Luk, HTL (ASCP) Cancer Research center of Hawaii Pathology shared resources lab manager KP- Hawaii Date: Wed, 8 Apr 2009 21:41:35 -0700 From: u_deserve_2_k...@yahoo.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet

RE: [Histonet] low profile blades and cutting angle

Stacey, Same for me too with Accuedge! But I also like Shandon MX35 Premier. Pack of 50 blades may be over $100 (depends on your Fisher pricing). I use these for both microtome and cyrostat sections. I've also used these for MMA sections. Premier Plus should be good

RE: [Histonet] Dako

I would like to ask how any of you feel about the Dako's Flex system. I'm having a hard time liking a system that is supposed to be a strong polymer, but needs a higher pH retrieval step to work. I also find myself tittering antibodies to a lesser degree. I should explain