Interesting comments. The use of this sample depends on if the antigen
survives fixation and paraffin embedding. About 45-55% of our work is done
with immunofluorescence on FFPE slides. we quench the autofluorescence with a
CuSO4 treatment.
James Watson HT ASCP
GNF Genomics Institute of
http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19930073077.pdf
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: Bitting
NASA did a lot of studies on this.
http://en.wikipedia.org/wiki/NASA_Clean_Air_Study
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From
I see that advertising is now allowed on the Histonet?
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: scott munday [mailto:mundaysc
I see that advertising is now allowed on the Histonet?
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: Klaus Dern [mailto:klaus.der
you have multiple slides to repair then they can be stacked.
Apply weight to the top of this and let stand overnight.
9. Soak the dry slide in Xylene until the Mount-Quick is removed and
coverslip as usual.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
% sucrose
first since brains flushed with PBS can have up to 20% shrinkage.
I have not heard of a good theory behind body stiffness after perfusion yet,
pretty sure it is not the effect of actual cross linking since that takes time.
Is someone knows the cause please let me know.
James Watson
whole brain once we
got near the cassette we would re-embed the brain so the reminder was out of
the cassette.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
Researchers
Should Know
http://www.clinchem.org/content/56/11/1675.full.pdf
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun
Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all
the labs at AFIP.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
antibodies?
Jamie
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel858-332-4647
Fax 858-812-1915<mailto:858-812-1915jwat...@gnf.org>
jwat...@gnf.org
___
Histonet m
switch the
containers to straight 100% reagent alcohol for them. For fat we have a longer
processing schedule and for cell pellets we have a short processing cycle.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858
This is at least the third time I have seen this message. Is this a form of
advertisement on the Histonet?
Grumpy in San Diego because it is supposed to rain today.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.e
g the whole counter
and wall catch fire.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwester
We use the plastic freezer boxes also and have our machine shop take out some
of the dividers to store the larger vials in.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Friday,
] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 1:57 PM
To: 'Emily Brown'
Cc: Histonet@Lists. Edu
Subject: RE: [Histonet] Ebola
Back in the 80 NIH did extensive studies on CJD, their protocol requires 24
hours in formalin, 24 hours in formic acid, followed by 48 hours i
publications I will
forward them.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
ierre E. Rollin, Special Pathogens Branch,
NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600
Clifton Road, Atlanta, GA 30333 (p...@cdc.gov).
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 8
Sorry forgot to add loner HIER damaged the Caspase 3 antigen.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun
needed FC receptor block
and permeablization. Ki67 needed longer HIER time than Caspase 3 so we did a
second short HIER after the Caspase 3 was labeled with DAB.
Jamie
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel
for 5 minutes in distilled water.
(7) Rinse in PBS 2 times for 10 minutes each.
(8) Coverslip slides with appropriate mounting media.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
uorescence. We use life
Technologies "Alexa Fluor" secondary antibodies.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
We have been using this Rat antibody in mouse tissue with both fluorescence and
Chromogen detection, The monoclonal antibody NIMP-R14 is highly specific for
murine Ly-6G and Ly-6C.
Neutrophil
Abcam
ab2557
http://www.abcam.com/neutrophil-antibody-nimp-r14-ab2557.html
James
With slides this old you can usually spray the back of the slide with freeze
spray and the coverslip will pop off. Then soak in xylene and once any
remaining permount is gone, re-coverslip. We did this routinely at AFIP, in
the old days.
James Watson HT ASCP
GNF Genomics Institute of the
. Do not use acetone or xylene,
they will dissolve the cement that holds the lens in place.
Jamie
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original
We are pharma research, about 40-45% of our slides are fluorescent. With 1
scanner.
201317,096 slides scanned
201320,501 slides scanned
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel
, very fast scanning with high quality scans. 60 slides per
hour.
There are many others on the market for smaller workloads.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel 858-332-4647
Fax 858-812-1915
jwat
not recycling
waste you are not practicing "waste management".
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
, the hanging
plants are doing quite well, so they make up for not seeing the light of day
somewhat.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun
… 0.0203 gm.
Manganese Chloride… 0.0125 gm.
Distilled Water……….…. 100.0 ml
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun
Olympus BH2 in
our Microtomy area.
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
Ventanas have worked great for us, but like all stainers they do have their
little idiosyncrasies.
Look at what fits your needs and look closely at the cost of running a system
vs. efficiency.
Jamie
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858
agarose using the modified spatula and wrap in lens paper.
3. Process the pellet on the normal mouse cycle on the tissue processor
using the 100% ETOH without the 5% glycerin.
4. Embed the pellet with the cultured cells at the bottom of the mold.
James Watson HT ASCP
GNF Genomics
tumor tissue, samples from benign lesions and normal tissues
should be frozen.(ABSTRACT TRUNCATED AT 250 WORDS)
PMID:
1342956
[PubMed - indexed for MEDLINE]
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
emission signal
noticeably
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On B
James Watson HT ASCP
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent
I was just informed that On Line registration is now working through our
website.
http://www.californiahistology.org/
I have also been told this can be accessed by smartphone, I-pad. also
payment can be made through PayPal or Credit Card.
James Watson HT ASCP
GNF Genomics Institute
coming from all over the
country with a wide variety of backgrounds and subjects. We are hoping to have
another great meeting. See you there.
Visit our website at to see the program and mail in registration form.
http://www.californiahistology.org/
James Watson HT ASCP
GNF Genomics
and he informed me that I
cost the navy about a half a million dollars in a lawsuit.
After that the pathologist insisted that all tissue was fully fixed and he did
not care how long he had to wait for his slides, he wanted them all perfectly
cut and stained perfectly.
James Watson HT ASCP
focus frequently. We are working on our mounting
technique to see if this can be corrected by modifying our coverslipping
technique.
Any suggestions would be appreciated. This does not make your fluorescence
permanent since most fluorochromes fade with age.
James Watson HT ASCP
GNF Genomics
whole dog heart cross
sections that took 48 hours to process. 2 minor problems with this: 1) does
not work on fat, so we keep extra containers of 100% ETOH to switch to when
processing fat. 2) we have to rinse our xylene recycling container and recycler
monthly with 100% ETOH.
James Watson HT
Our last order to dianova was switched to their new US distributor:
http://www.histobiotec.com/home.php?cat=
check with them
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
derly.
7. Operate Slide scanning Instrumentation.
Interested candidates please send a copy of your CV with a summary of research
experience, and three references.
Job Code: JW10-013
Contact:E-mail: j...@gnf.org<mailto:j...@gnf.org>
James Watson HT ASCP
Facilities Manager of Hi
ly.
7. Operate Slide scanning Instrumentation.
Interested candidates please send a copy of your CV with a summary of research
experience, and three references.
Job Code: JW10-013
Contact:E-mail: j...@gnf.org<mailto:j...@gnf.org>
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomic
Testing other messages have not been posted
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel858-332-4647
Fax 858-812-1915<mailto:858-812-1915jwat...@gnf.org>
jwat...@g
I just got some of this antibody in an will be setting it up on the Ventana
Discovery Thank you for the information on your protocol. I will let you know
how it goes.
Jamie
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel
We routinely quench RBC auto-fluorescence with the following procedure:
10 mM Copper Sulfate
10 mM Copper Sulfate
Cupric Sulfate...1.25 gm
50 mM Amonium acetate (pH5)500.0 ml
Adjust ph to 5.0 with 1.0 M NaOH
50 mM Ammonium acetate (pH5)
Here is a link to a list based on a NASA studies for plants that absorb
benzene, formaldehyde, trichloroethylene, xylene, and toluene.
http://en.wikipedia.org/wiki/List_of_air-filtering_soil_and_plants
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the
E
Please submit your CV and any supporting documents to:
Genomics Institute of the Novartis Research Foundation Job Code: JW09-025
10675 John Jay Hopkins Drive
San Diego, CA 92121
Fax: 858/812-1670, or submit online to j...@gnf.org<mailto:j...@gnf.org>
(subject line must include
water.
8. Rinse in PBS 2 times for 10 minutes each.
9. Coverslip slides with appropriate mounting media.
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915
jwat...@gnf.org
-Original
Formaldehyde Fixation0022-1554/85/83. 30. The. Journal of Histochemistry
and Cytochemistry. Copyright ... U.S.A.. Formaldehyde. Fixation. Review.
Article. CECIL. H. FOX, ...
www.jhc.org/cgi/reprint/33/8/845.pdf - Similar pages
by CH Fox - 1985
-Original Message-
From: histonet-boun...@list
Would you like to spend the summer in Sunny San Diego Ca.?
Job Description
GNF is currently seeking a temporary Scientific Associate to join the
Histology group (End of June 2009 through beginning of October 2009).
Job Summary
Performs routine, special staining and complex procedures nec
We use Zamboni's fixative and are able to use paraffin sections. We do
use 5% glycerin in our 100% denatured alcohol so that might be
protecting the GFP from being damaged during dehydration.
jwatson
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Emily
they would make
by short dating expiration dates on an antibody. This idea of a "stability
guarantee date" might relieve the manufacturer of responsibility and place it
on the technician, pathologist and lab.*
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Instit
2, 2008.
R.S.V.P. [EMAIL PROTECTED]
If you are interested in becoming a member of the San Diego chapter,
please contact me.
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel858-332-4647
Fax 858-812-1915 <
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