[Histonet] Precision in IHC validations

2024-04-16 Thread Martha Ward-Pathology via Histonet
Thanks to everyone that has responded to my question. We specifically interested in the procedure for this CAP question: COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/ approved Tests and LDTs Prior to clinical use of each modified FDA-cleared or approved test

[Histonet] How are you documenting precision in IHC validations

2024-04-16 Thread Martha Ward-Pathology via Histonet
We are working on IHC antibody validations and I want to know if there is a minimum number of slides you must run to demonstrate precision? I have not been able to find a definitive number or procedure. What is everyone doing for this? Thanks in advance for everyone's help. Martha Ward

[Histonet] Bone marrow clot IHC tissue sections washing

2021-09-23 Thread Martha Ward-Pathology via Histonet
of positive charged slide with good results for several years and rarely have issues with other tissue types unless they are particularly bloody. Thoughts or suggestions are greatly appreciated. Martha Ward MT(ASCP) QIHC Atrium Health Wake Forest Baptist

[Histonet] antibody protocol for CXCL13

2021-08-24 Thread Martha Ward-Pathology via Histonet
Our heme path docs are interested in this antibody and I wondered if anyone has a protocol they can share?We have the Leica Bond 3 as our staining platform/detection system. Thanks in advance for your help. Martha Ward MT(ASCP) QIHC Manager, Molecular Diagnostics Lab Atrium Health Wake

Re: [Histonet] HSV1 and HSV2

2021-06-15 Thread Martha Ward-Pathology via Histonet
Thank you for the information. I will look into your antibody source. Martha -Original Message- From: Cartun, Richard Sent: Tuesday, June 15, 2021 3:26 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: HSV1 and HSV2 Hi Martha: We are using

[Histonet] HSV1 and HSV2

2021-06-15 Thread Martha Ward-Pathology via Histonet
. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Filed slides sticking together

2021-04-16 Thread Martha Ward-Pathology via Histonet
I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha Ward, MT ASCP

[Histonet] Antibody validation question

2021-03-01 Thread Martha Ward-Pathology via Histonet
When doing an initial antibody validation CAP requires at least 90% concordance between the new test and the comparator test or expected values.Are there specified requirements for sensitivity, specificity and accuracy? Thanks in advance for any help provided! Martha Ward, Manager

[Histonet] Question about accessioning outside consult cases

2020-12-10 Thread Martha Ward-Pathology via Histonet
S20-? We currently assign them as just another surgical case but I know some places do have outside consult designations. What is everyone doing? Thanks in advance for your input. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston

[Histonet] "Floaters" in surgical or cytology specimens

2020-12-04 Thread Martha Ward-Pathology via Histonet
for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue processor validation

2020-09-02 Thread Martha Ward-Pathology via Histonet
in validating a new processor. Current ones are 25+ years. Any help or advice would be appreciated. Thanks in advance! Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] IHC validation after a new tissue processer is installed

2020-09-01 Thread Martha Ward-Pathology via Histonet
with this. Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mounting media for immunofluorescence slides

2020-03-06 Thread Martha Ward-Pathology via Histonet
on the slides? We plan to test a few control slides to see how they look. Thanks in advance for any advice you can give me. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet

[Histonet] PPE for the Histology lab

2019-09-09 Thread Martha Ward-Pathology via Histonet
on this issue. Martha Ward Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RUO vs IVD antibodies in immunofluorescence procedures

2019-04-08 Thread Martha Ward-Pathology via Histonet
We are looking for IVD antibodies for C1q and C4d for use on frozen tissues (renal). What vendors are folks using for these direct immunofluorescence stains? Thanks in advance for your help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157

[Histonet] Looking for vendor for Zeus Wash solution

2019-03-21 Thread Martha Ward-Pathology via Histonet
, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Vendors closed on Presidents Day

2019-02-18 Thread Martha Ward-Pathology via Histonet
have someone staffing the number to cover issues that come up. Thankfully my call isn't urgent but it could have been and then I would be stuck. Martha Ward Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Reproducibility in IHC validations

2018-11-16 Thread Martha Ward-Pathology via Histonet
in advance for your help. Martha Ward Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HPV testing on head and neck tumors and reimbursement

2018-01-22 Thread Martha Ward-Pathology via Histonet
. This is for CPT code 87624. Thank you in advance for any input you may have on this issue. Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Medical Center Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Recycling formalin

2017-12-20 Thread Martha Ward-Pathology via Histonet
questions about unstained slides for prostate biopsies.It was very helpful. Thanks in advance for your help with our recycled formalin question. Martha Ward Wake Forest Baptist Health 336-716-2109 ___ Histonet mailing list Histonet

[Histonet] Prostate needle biopsies

2017-12-15 Thread Martha Ward-Pathology via Histonet
. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 25157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Beaker with or without Vantage

2017-11-28 Thread Martha Ward-Pathology via Histonet
"They" aren't the ones that will have to use it.therein lies the rub! I have not heard many positive things about AP Beaker.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716

[Histonet] Looking for a C4d ffpe control block

2017-03-29 Thread Martha Ward-Pathology via Histonet
be in need of as well. Thanks in advance for everyone's help. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 mw...@wakehealth.edu<mailto:mw...@wakehealth.edu> ___ Histonet mailin

[Histonet] Merkel cell polyomavirus antibody

2017-01-27 Thread Martha Ward-Pathology via Histonet
Good morning, I have had a request for this antibody but I am having trouble locating a vendor for it.If anyone has any information on vendor, etc. , I would appreciate it. We have Leica Bond 3s. Thank you in advance for your help. Martha Ward Wake Forest Baptist Health

[Histonet] New IHC instrument validation process

2016-11-16 Thread Martha Ward-Pathology via Histonet
of each antibody to demonstrate that the new instrument compares to the old.The detection kits, retrieval solutions, antibodies, etc. would all remain the same. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157

[Histonet] Looking for a maintenance form

2016-10-03 Thread Martha Ward-Pathology via Histonet
to reinvent the wheel and would like to see what others are doing. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 336-716-2109 ___ Histonet mailing list Histonet

[Histonet] Dako Herceptest Link kit question

2016-09-07 Thread Martha Ward-Pathology via Histonet
concerned about the reagents spreading evenly across the slide. What is everyone else's experience with this issue? Thanks in advance for your help! Martha Ward Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] PLA2R assay question

2016-05-02 Thread Martha Ward-Pathology via Histonet
this via traditional IHC (we have Bond 2 stainers).Is anyone doing this way? If so, could you provide some tips on getting it to work successfully? Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC

Re: [Histonet] Blog Post Not lab related

2016-04-14 Thread Martha Ward-Pathology via Histonet
I agreethe delete button is very easy to use. -Original Message- From: Jennifer MacDonald via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, April 14, 2016 12:16 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not

Re: [Histonet] Billing

2015-05-29 Thread Martha Ward-Pathology
Yes that is how we do it as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: Vickroy, James [mailto:jvick

Re: [Histonet] IHC billing question

2015-05-01 Thread Martha Ward-Pathology
That's what we do as well. Martha Ward Wake Forest Baptist Health From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Friday, May 01, 2015 9:13 AM To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing

[Histonet] RE: IHC Billing Question

2015-04-16 Thread Martha Ward-Pathology
We have not been charging for the negative control, assuming that it was just a cost of doing business. I would be interested to hear if anyone has been charging for their negative controls as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard

[Histonet] Her2 IHC validation

2015-01-22 Thread Martha Ward-Pathology
been unable to find one in my reading. Thank you in advance for your help.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu

RE: [Histonet] p16 Antibody

2015-01-14 Thread Martha Ward-Pathology
We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message

[Histonet] back orders from various vendors

2014-12-02 Thread Martha Ward-Pathology
expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems . Thanks for letting get this off my chest.I will resume my frantic search for another antibody vendor now. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab

[Histonet] RE: back orders from various vendors

2014-12-02 Thread Martha Ward-Pathology
for the inconvenience. Argg -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Tuesday, December 02, 2014 10:59 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: back orders from various vendors Happens every year at this time with various

[Histonet] RE: EDTA decalcification solution follow up questions

2014-12-02 Thread Martha Ward-Pathology
? Thanks again for any information   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun

[Histonet] EDTA decalcification solution

2014-12-01 Thread Martha Ward-Pathology
about how you use itlength of time in solution, etc. Thanks in advance for your help.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu

[Histonet] RE: questions

2014-08-07 Thread Martha Ward-Pathology
Sounds like we are all working at the same place! LOL I hear exactly the same questions.is it something they teach to all pathology residents   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f

[Histonet] RE: rolling sections

2014-06-12 Thread Martha Ward-Pathology
We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw

RE: [Histonet] Re: Negative Controls

2014-04-30 Thread Martha Ward-Pathology
So do we.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] CAP question ANP.22978 - Her2 assay validation

2014-04-29 Thread Martha Ward-Pathology
on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 ___ Histonet mailing list Histonet

[Histonet] RE: Instrument Verification

2014-04-03 Thread Martha Ward-Pathology
I'm with you. There really appears to be no value to this particular requirement.I would only be concerned with it if I had just purchased it, or moved it into our lab from another location.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard

RE: [Histonet] RE: Negative Controls for IHC

2014-02-12 Thread Martha Ward-Pathology
We discontinued them over a year ago.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Turn Around Time

2014-02-04 Thread Martha Ward-Pathology
on our PCR testing as those are batched. More of an editorial comment that anything else Martha From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Monday, February 03, 2014 4:38 PM To: Martha Ward-Pathology; Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Turn Around Time

RE: [Histonet] Turn Around Time

2014-02-03 Thread Martha Ward-Pathology
shoots for the 80% in 24 hours as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: HER2/NEU

2014-01-15 Thread Martha Ward-Pathology
That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center

[Histonet] Vendor suggestions for IgG subclass antibodies

2013-11-19 Thread Martha Ward-Pathology
in advance for your help!   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       ___ Histonet mailing list Histonet

[Histonet] cell block staining issues

2013-10-31 Thread Martha Ward-Pathology
in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list

[Histonet] RE: cell block staining issues

2013-10-31 Thread Martha Ward-Pathology
the same doctor/clinic and see if they are doing anything different. Martha -Original Message- From: Tom McNemar [mailto:tmcne...@lmhealth.org] Sent: Thursday, October 31, 2013 9:17 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: cell block staining issues We have

[Histonet] RE: Submitting Questions

2013-09-24 Thread Martha Ward-Pathology
I have had the same problem over the past few months...   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun

[Histonet] Minimum sample size for breast markers

2013-09-12 Thread Martha Ward-Pathology
...20 tumor cells, 50 tumor cells Any information would be appreciated. Thanks in advance for your help.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu

RE: [Histonet] Minimum sample size for breast markers

2013-09-12 Thread Martha Ward-Pathology
Thanks everyone From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, September 12, 2013 10:56 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers No, there are no written instructions on the subject. You just

RE: [Histonet] Re: Minimum sample size for breast markers

2013-09-12 Thread Martha Ward-Pathology
Thanks to everyone that responded to my question. It has encouraged a nice discussion and I have learned from it.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu

RE: [Histonet] EPIC Beaker

2013-09-04 Thread Martha Ward-Pathology
I would be interested in any information as well. We do not plan to implement for a year or more but one of our hospitals is working on Beaker now and finding that it does not adapt to AP as well as it does CP.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center

[Histonet] Immunofluorescence on paraffin sections

2013-08-28 Thread Martha Ward-Pathology
give me. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Unstained slides

2013-08-28 Thread Martha Ward-Pathology
are never used and we are trying to find out if that is high, low or about what other institutions are seeing. If anyone has any data they could share with us we would appreciate it. Thanks in advance for your help. I can share what I find out if others are interested. Martha Ward, MT

[Histonet] DAB enhancer

2013-07-11 Thread Martha Ward-Pathology
I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks!   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab

[Histonet] DAB enhancer

2013-07-11 Thread Martha Ward-Pathology
and what are the pros and cons of its use? Thanks! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message

RE: [Histonet] Billing ???

2013-06-21 Thread Martha Ward-Pathology
At our institution the hospital bills the technical and the pathologist bills the professional.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original

[Histonet] p16 on the Bond

2013-06-21 Thread Martha Ward-Pathology
, without much success. Thanks in advance for the help.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       ___ Histonet

[Histonet] RE: negative controls

2012-08-17 Thread Martha Ward-Pathology
We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run 10 negative slides.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw

RE: [Histonet] Negative Reagent Control

2012-07-18 Thread Martha Ward-Pathology
What wonderful news   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Beaker module in EPIC

2012-07-13 Thread Martha Ward-Pathology
Beaker? Martha Ward Wake Forest Baptist Health 336-716-2109 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, July 12, 2012 4:05 PM To: HistoNet; microsc...@microscopy.com Subject

RE: [Histonet] Beaker module in EPIC

2012-07-13 Thread Martha Ward-Pathology
So far we have not had any responses so I assume the answer is no.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: Michael

RE: [Histonet] Epic Beaker module

2012-07-12 Thread Martha Ward-Pathology
We would interested in any replies as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun

[Histonet] Napsin A

2012-04-24 Thread Martha Ward-Pathology
Hello. I was wondering if anyone had used the NapsinA from BioCare on the Bond III and if so, would you mind sharing your protocol? Thanks in advance for any advice you can give. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist

RE: [Histonet] NCCI policy on IHC billing

2012-01-05 Thread Martha Ward-Pathology
From what we understand from the new policy the cocktailed antibodies, such as PIN4, are not to be charged separately. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104

[Histonet] RE: Epic LIS

2012-01-04 Thread Martha Ward-Pathology
I would also be interested in any replies concerning EPIC-beaker. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun

[Histonet] RE: NCCI policy update

2011-12-30 Thread Martha Ward-Pathology
Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun

[Histonet] C4d immunofluorescent positive controls

2011-12-29 Thread Martha Ward-Pathology
We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest

[Histonet] PAX 2 antibody

2011-12-12 Thread Martha Ward-Pathology
Hello all, I am still hunting for a source for the PAX 2 antibody.Is this antibody currently available? I have heard in the past that there was a manufacturing issue. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical

[Histonet] MHC-class 1 antibody

2011-11-23 Thread Martha Ward-Pathology
tissue but they are looking for an antibody for paraffin embedded tissues. Any help would be appreciated. Hope everyone has a good Thanksgiving holiday. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem

[Histonet] IDH1 and IDH2 antibodies, MHC-Class I

2011-11-21 Thread Martha Ward-Pathology
Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest

[Histonet] RE: Leica Bond III

2011-09-02 Thread Martha Ward
We sometimes have issues with EBER and more often, with the Kappa and Lambda ISH on the Bond 3, more specifically on our tissues that were fixed in B Plus rather than formalin. I would interested in the responses as well. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept

[Histonet] Test email after email address change

2011-09-01 Thread Martha Ward
Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] C FLIP AND NKF Beta antibodies

2011-07-19 Thread Martha Ward
I have been asked about a research project involving these two antibodies and we are having some trouble finding sources that are appropriate for formalin fixed, paraffin embedded tissues. Does anyone have experience with these antibodies? Martha Ward, MT (ASCP) QIHC Assistant Manager

[Histonet] RE: Lab humidity range

2011-07-14 Thread Martha Ward
We chose 10 - 90% as our range after reading what few instrument manuals even addressed the issue. I can only wish we could get to 17% humidity here in NC. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center

[Histonet] Langerin antibody

2011-07-12 Thread Martha Ward
you would need. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list

[Histonet] cost to produce one block

2011-05-19 Thread Martha Ward
is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct.Any responses will be kept confidential. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept

[Histonet] Thanks for the hair processing information

2011-05-10 Thread Martha Ward
Thanks so much to the folks that responded to my request for information. I forwarded them on to my colleague. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104

[Histonet] Processing hair

2011-05-09 Thread Martha Ward
I am posting this question for a colleague who has been approached to do a research project involving processing human hair. Has anyone done this, and if so, could you provide the procedure. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics

[Histonet] Direct costs for small biopsy processing

2011-03-04 Thread Martha Ward
to accession the case, the tissue processing, embedding, microtomy, staining the HE, coverslipping and reviewing the slide. We know that reagents and salary costs will vary but she just wants a ball park figure for comparison.Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC

[Histonet] SOX11 antibody

2011-03-03 Thread Martha Ward
. Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet

[Histonet] Her2 requests on decal specimens

2011-02-10 Thread Martha Ward
this? Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] IHC validation

2011-02-09 Thread Martha Ward
What a sensible process! I agree with your approach. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun

RE: [Histonet] High Complexity Testing

2011-02-08 Thread Martha Ward
I completely agree!!! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] RE: Manual Coverslipping Safety Issues

2011-01-04 Thread Martha Ward
a world of difference. Good luck! Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Fisher MicroProbe manual staining system

2010-12-22 Thread Martha Ward
Does anyone have one of these systems that they would be willing to donate? One of our pathologists is going on a mission trip to Honduras and would like to take one of these down there with him. We used to have the set up but recently got rid of it. (Timing is everything!!) Martha Ward, MT

[Histonet] RE: Physician Signatures

2010-12-17 Thread Martha Ward
Like everyone else we are struggling with how to get everyone to comply. If anyone has any plans I think the whole group could benefit. Right now our institution is getting together to figure out a way to get the information out to the surgeons, etc but no concrete plans. Martha Ward, MT

RE: [Histonet] CAP question ANP.22970

2010-10-21 Thread Martha Ward
We are doing it by hand as well, into a spread sheet, and then doing the calculations. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 -Original Message- From: histonet-boun

[Histonet] paperless requisitions

2010-10-21 Thread Martha Ward
reference labs. Is anyone out there currently paperless? We would be interested in seeing how you accomplished it, any pitfalls, examples of your on-line req, etc. Thanks in advance for any responses. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University

[Histonet] C4d on paraffin sections

2010-08-13 Thread Martha Ward
! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Phospho-Histone 3 (SER10) antibody

2010-07-12 Thread Martha Ward
. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] p63 antibody

2010-05-18 Thread Martha Ward
This may have been discussed before butnow that Dako is no longer offering p63, which vendor are most of you switching to? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104

RE: [Histonet] p63

2010-05-18 Thread Martha Ward
Thanks but the memo I received dated May 10th stated that Dako's license to sell M7247, p63 antibody, was due to expire on 31 May 2010. That was why I was looking for a new vendor. Thank you to everyone who responded to my request. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular

[Histonet] IgG4

2010-03-01 Thread Martha Ward
Hello all, I am looking for a lab that offers this antibody on ffpe tissues. One of my Pathologists is interested in it and has a surgical case she has been asked to stain for IgG4. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab

[Histonet] FITC for IgG 1,2,3,4

2010-02-19 Thread Martha Ward
I have a request from a pathologist for these antibodies. I have been unable to locate a vendor in my usual places. Is anyone doing these antibodies, and if so, could you let me know a vendor (s) to check out? Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager

[Histonet] tech schedules

2009-12-01 Thread Martha Ward
responses to him. Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathologyreports

2009-11-20 Thread Martha Ward
We use the statement for all reports that any IHC/ISH, regardless if they are ASRs or not. Martha Ward Wake Forest University Baptist Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Foshey

  1   2   >