[Histonet] Precision in IHC validations

Thanks to everyone that has responded to my question. We specifically interested in the procedure for this CAP question: COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/ approved Tests and LDTs Prior to clinical use of each modified FDA-cleared or approved test

[Histonet] How are you documenting precision in IHC validations

We are working on IHC antibody validations and I want to know if there is a minimum number of slides you must run to demonstrate precision? I have not been able to find a definitive number or procedure. What is everyone doing for this? Thanks in advance for everyone's help. Martha Ward

[Histonet] Bone marrow clot IHC tissue sections washing

It was brought to my attention that we had significant washing on 3 of 8 bone marrow clot sections the other day; this is not the first time so we would like to get to the bottom of this. We use positively charged slides and all 8 cases were cut and run the same morning but allowed to air

[Histonet] antibody protocol for CXCL13

Our heme path docs are interested in this antibody and I wondered if anyone has a protocol they can share?We have the Leica Bond 3 as our staining platform/detection system. Thanks in advance for your help. Martha Ward MT(ASCP) QIHC Manager, Molecular Diagnostics Lab Atrium Health Wake

Re: [Histonet] HSV1 and HSV2

Thank you for the information. I will look into your antibody source. Martha -Original Message- From: Cartun, Richard Sent: Tuesday, June 15, 2021 3:26 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: HSV1 and HSV2 Hi Martha: We are using

[Histonet] HSV1 and HSV2

We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help.

[Histonet] Filed slides sticking together

I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha Ward, MT ASCP

[Histonet] Antibody validation question

When doing an initial antibody validation CAP requires at least 90% concordance between the new test and the comparator test or expected values.Are there specified requirements for sensitivity, specificity and accuracy? Thanks in advance for any help provided! Martha Ward, Manager,

[Histonet] Question about accessioning outside consult cases

We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20- verses

[Histonet] "Floaters" in surgical or cytology specimens

I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance

[Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab. She is working on a plan for a new tissue processor validation, including special stains, and would like to get input from anyone that has already gone through it.This is the first time anyone here has ever been involved in

[Histonet] IHC validation after a new tissue processer is installed

Hello all, After a very long time our histology department is getting a new tissue processor, and hopefully several more in the near future. Of course for IHC that means a revalidation/reverification of our IHC stains. Since I have not had to do this before I wanted to get some guidance on

[Histonet] Mounting media for immunofluorescence slides

This may be an odd request but I am hoping someone out there can help us with this problem. Our institution is purchasing new digital scanners (Olympus 200) to scan all our pathology case slides. It has come to our attention that the mounting media we have all been using for cover

[Histonet] PPE for the Histology lab

Our department is looking at safety issues and one suggestion from our CLIA director is that everyone in the lab (our lab performs IHC only) should wear gloves, a gown and eye or face protection at all times and requiring it for anyone that enters the lab. We have task specific PPE

[Histonet] RUO vs IVD antibodies in immunofluorescence procedures

We are looking for IVD antibodies for C1q and C4d for use on frozen tissues (renal). What vendors are folks using for these direct immunofluorescence stains? Thanks in advance for your help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157

[Histonet] Looking for vendor for Zeus Wash solution

I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies.Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks,

[Histonet] Vendors closed on Presidents Day

Am I the only one that finds it odd and frustrating that some vendors are closed on Presidents Day? I just tried to call the technical service number for Dako about a problem and the message said to call back tomorrow! Hospital labs don't close and I would have thought they would at least

[Histonet] Reproducibility in IHC validations

My medical director and I are working on our procedure for reproducibility in our IHC validations and would like to know how others are handling this. How many slides do you run and over how many days/runs?If someone would share their procedure with us that would be wonderful. Thanks in

[Histonet] HPV testing on head and neck tumors and reimbursement

Hello, My billing group wanted me to ask if anyone is seeing denials for reimbursement for HPV testing on male Medicare patients when it is performed on ffpe for head and neck tumors? We have seen a few denials recently and they wanted to see how everyone is handling the coding. This is

[Histonet] Recycling formalin

I am posting this for our Histology lab manager: Is anyone recycling formalin and if so, exactly what are you using the recycled formalin for?Putting it on processors, adding to specimens, etc? Apparently our EHS department is pushing this. Also thank everyone for their help with our

[Histonet] Prostate needle biopsies

I am posting this question for our Histology manager.For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut? Thanks in advance for everyone's help.

Re: [Histonet] Beaker with or without Vantage

"They" aren't the ones that will have to use it.therein lies the rub! I have not heard many positive things about AP Beaker.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890  

[Histonet] Looking for a C4d ffpe control block

Hello everyone, I am trying to find someone who can share a C4d ffpe control block with us. Ours is almost gone and I don't have access to any suitable tissues in house. If anyone has a block they are willing to share please contact me. I can try to help out with any controls that you may

[Histonet] Merkel cell polyomavirus antibody

Good morning, I have had a request for this antibody but I am having trouble locating a vendor for it.If anyone has any information on vendor, etc. , I would appreciate it. We have Leica Bond 3s. Thank you in advance for your help. Martha Ward Wake Forest Baptist Health

[Histonet] New IHC instrument validation process

How is everyone handling new instrument validation processes? We are looking at getting a new immunostainer.Beyond the varication process that the installers do what are our responsibilities from a lab stand point? We have a large menu of antibodies.Would it be sufficient to run one

[Histonet] Looking for a maintenance form

Does anyone have a specific form that they use to indicate that a particular instrument is functioning as expected after service repairs or PMs? We need to document this information and I would like a simple form to fill out and include with the service reports.Just trying to not have to

[Histonet] Dako Herceptest Link kit question

We just installed a new Dako Link Autostainer and have started using the Herceptest Link kit. We had been using two drop zones with our other, older instrument but that was applying only 100 uL in the 2 drop zones. With this new program we set it up to apply two zones of 200 uL but

[Histonet] PLA2R assay question

I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure.I would prefer to do this

Re: [Histonet] Blog Post Not lab related

I agreethe delete button is very easy to use. -Original Message- From: Jennifer MacDonald via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, April 14, 2016 12:16 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not

Re: [Histonet] Billing

Yes that is how we do it as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: Vickroy, James

Re: [Histonet] IHC billing question

That's what we do as well. Martha Ward Wake Forest Baptist Health From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Friday, May 01, 2015 9:13 AM To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing

[Histonet] RE: IHC Billing Question

We have not been charging for the negative control, assuming that it was just a cost of doing business. I would be interested to hear if anyone has been charging for their negative controls as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \ 

[Histonet] Her2 IHC validation

In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates.We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have

RE: [Histonet] p16 Antibody

We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message-

[Histonet] back orders from various vendors

I just need a moment to ventis it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not

[Histonet] RE: back orders from various vendors

for the inconvenience. Argg -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Tuesday, December 02, 2014 10:59 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: back orders from various vendors Happens every year at this time with various

[Histonet] RE: EDTA decalcification solution follow up questions

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, December 01, 2014 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution I am asking this question for our Histology Lab. They are being asked about

[Histonet] EDTA decalcification solution

I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure

[Histonet] RE: questions

Sounds like we are all working at the same place! LOL I hear exactly the same questions.is it something they teach to all pathology residents   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f

[Histonet] RE: rolling sections

We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890  

RE: [Histonet] Re: Negative Controls

So do we.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] CAP question ANP.22978 - Her2 assay validation

Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into

[Histonet] RE: Instrument Verification

I'm with you. There really appears to be no value to this particular requirement.I would only be concerned with it if I had just purchased it, or moved it into our lab from another location.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \ 

RE: [Histonet] RE: Negative Controls for IHC

We discontinued them over a year ago.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Turn Around Time

on our PCR testing as those are batched. More of an editorial comment that anything else Martha From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Monday, February 03, 2014 4:38 PM To: Martha Ward-Pathology; Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Turn Around Time

RE: [Histonet] Turn Around Time

While I recognize the need for a quick result in some cases, I also subscribe to the theory that speed kills. I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.However that is another discussion altogether. That said, our institution

[Histonet] RE: HER2/NEU

That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest.I would be interested in hearing others responses as well. Thank you for asking the question.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center

[Histonet] Vendor suggestions for IgG subclass antibodies

I am looking for a vendor for FITC labeled IgG1, IgG2 IgG3 and IgG4 antibodies for use in our immunofluorescence panels when staining renal biopsies. We get most of our FITC antibodies for our panel from Dako but I didn't see these others. Any suggestions are appreciated. Thanks in

[Histonet] cell block staining issues

We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).The

[Histonet] RE: cell block staining issues

the same doctor/clinic and see if they are doing anything different. Martha -Original Message- From: Tom McNemar [mailto:tmcne...@lmhealth.org] Sent: Thursday, October 31, 2013 9:17 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: cell block staining issues We have

[Histonet] RE: Submitting Questions

I have had the same problem over the past few months...   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From:

[Histonet] Minimum sample size for breast markers

Is there a minimum number of cells that should be present when testing for ER, PR and Her2? I have looked on the CAP website but cannot see anything about this. We are constantly getting these small biopsies or FNAs for testing and I sometimes wonder what the lowest threshold is for

RE: [Histonet] Minimum sample size for breast markers

Thanks everyone From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, September 12, 2013 10:56 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Minimum sample size for breast markers No, there are no written instructions on the subject. You just

RE: [Histonet] Re: Minimum sample size for breast markers

Thanks to everyone that responded to my question. It has encouraged a nice discussion and I have learned from it.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu      

RE: [Histonet] EPIC Beaker

I would be interested in any information as well. We do not plan to implement for a year or more but one of our hospitals is working on Beaker now and finding that it does not adapt to AP as well as it does CP.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center

[Histonet] Immunofluorescence on paraffin sections

We do quite a number of renal biopsies and sometimes the frozen tissue is not sufficient for the immunofluorescent panel and we have to use paraffin sections for our fluorescence staining. It has never really worked all that well and so we are looking for alternative methods. We get all

[Histonet] Unstained slides

We are looking at ways to improve our work processes, save time and labor and reduce costs, all while maintaining patient quality...as we all are of course. During our conversations the subject of cutting unstained slides has come up and we are looking for bench marking data to see if we are

[Histonet] DAB enhancer

I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and what are the pros and cons of its use? Thanks!   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab

[Histonet] DAB enhancer

Sorry but I cannot get my messages to go through to the Histonet so I am piggybacking onto a previous message: I have been asked by a new pathologist to look into a DAB enhancer for use on our Bond III instruments. Are many of you using this on the Bond, or any other staining platform and

RE: [Histonet] Billing ???

At our institution the hospital bills the technical and the pathologist bills the professional.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original

[Histonet] p16 on the Bond

I am having trouble posting to the histonet for some reason. I am looking for condition and incubation times for p16 on the Bond. Our new pathologist says our p16 looks overstained to him and not at all what he is accustomed to seeing and I am trying to get the staining more to his liking,

[Histonet] RE: negative controls

We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run 10 negative slides.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890  

RE: [Histonet] Negative Reagent Control

What wonderful news   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Beaker module in EPIC

Again, we would be interested in any replies as well. Our institution is going live with Epic this fall but the labs will not be using Beaker until later on. We are still trying to figure out how all this will work and welcome all information. Paula, how soon will Duke be up and using

RE: [Histonet] Beaker module in EPIC

Mihalik [mailto:m...@pathview.com] Sent: Friday, July 13, 2012 2:03 PM To: Martha Ward-Pathology; 'Paula Sicurello'; 'HistoNet'; microsc...@microscopy.com Subject: RE: [Histonet] Beaker module in EPIC I'm curious. Are ANY labs using Epic for their APLIS? The last I heard was a sales

RE: [Histonet] Epic Beaker module

We would interested in any replies as well.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890   mw...@wakehealth.edu       -Original Message- From:

[Histonet] Napsin A

Hello. I was wondering if anyone had used the NapsinA from BioCare on the Bond III and if so, would you mind sharing your protocol? Thanks in advance for any advice you can give. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist

RE: [Histonet] NCCI policy on IHC billing

From what we understand from the new policy the cocktailed antibodies, such as PIN4, are not to be charged separately. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104

[Histonet] RE: Epic LIS

I would also be interested in any replies concerning EPIC-beaker. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From:

[Histonet] RE: NCCI policy update

Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From:

[Histonet] C4d immunofluorescent positive controls

We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest

[Histonet] PAX 2 antibody

Hello all, I am still hunting for a source for the PAX 2 antibody.Is this antibody currently available? I have heard in the past that there was a manufacturing issue. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical

[Histonet] MHC-class 1 antibody

Thank you to the people who responded to my inquiry about the IDH1 antibody. I think we have found one to try. I have also have a request for the MHC-Class 1 antibody for muscle biopsies. Could anyone performing this suggest a vendor. So far I am coming up with antibodies for frozen

[Histonet] IDH1 and IDH2 antibodies, MHC-Class I

Hello all, I have had a request from our neuropathologists to bring these antibodies in house but I am having some trouble finding vendors for them. I would appreciate any information. Thanks! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest