Jay,
I would think that the alkaline properties of the ammonia slow the swelling of
the tissues unlike plain water.
Sincerely,
Toysha N. Mayer, DHSc, MBA, HT (ASCP)
Asst Professor/ Assoc Program Director
HTL Program
UTMDACC
tnma...@mdanderson.edu
off cell: 832-710-1837
off: 713-563-3481
* My w
Hi Ken,
Try mushrooms.
Sincerely,
Toysha N. Mayer, DHSc, MBA, HT (ASCP)
Asst Professor/ Assoc Program Director
HTL Program
UTMDACC
tnma...@mdanderson.edu
off cell: 832-710-1837
off: 713-563-3481
Today's Topics:
1. Aspergillus (Ken M)
-
Charles,
I am going to date myself and tell you that we placed the glass coplin jar on
the back of the embedder (or in the water bath) added a bankers light and
wrapped it with foil for 1 hr.
Sincerely,
Toysha N. Mayer, DHSc, MBA, HT (ASCP)
Asst Professor/ Assoc Program Director
HTL Program
UT
Have HVAC check the temp in the center of the room as well as by the vents. We
had a problem that the equipment produced so much heat that it caused the
ribbons to stick together.
Using regulations for instrument and reagent storage can validate your request.
I would also take into account the
Another thing that can be done is to donate them to your closest histology
program. If they have a student lab, blocks are always needed.
If you facility is a teaching hospital, you may already be covered under the
initial waiver for uses for teaching, or whatever it's called. Some programs
wi
I would check the roller to see if it is making good contact and the slide
holder track as well. Not sure it that is what it is called, but where the
slide is pushed under the roller.
Sincerely,
Toysha N. Mayer, DHSc, MBA, HT (ASCP)
Assistant Professor/Associate Program Director
HTL Program S
Hi Jordan,
So wow! You are doing a Jones with an H&E.
What you are seeing is common when using a water bath and preheating. While
you can perform the stain with this many sections, I would start off with 1-3
slides until you have perfected the technique.
I know that makes you have multiple run
Hey Betsy,
It could also be the woodstain scarlet and the saffron. I would omit the 5%
acetic after the phosphotungstic.
Take care,
Toysha Mayer
Message: 1
Date: Fri, 7 May 2021 22:11:30 +
From: John Kiernan
To: "histonet@lists.utsouthwestern.edu"
,Betsy Molinari
Hi Jay,
It did not take the full 45 days for my students when they applied in June.
But those who applied later did have to wait longer than those who applied
earlier. I don't any of them took 45 days though. It may just be the review
of your submitted information.
Toysha
Message: 1
Date
Vikki,
I teach students that they should not have any air bubbles in their blocks.
This means those under the lip of the cassette as well as surrounding the
tissues. Air bubbles can cause instability in the block especially if the
tissue is hard or very small. When they go to a clinical rota
Pam,
My advice would be to remember that they should first master their entry-level
competencies before focusing on advanced techniques. The ability to master
those techniques prove invaluable when taking the BOC and maintaining
employment.
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Assistant Pr
Laurie,
The warm water helps the color of the Schiff develop in a more vibrant pattern.
If you allow the slides to sit in warm water for 2 min, then rinse you also do
not have to use the metabisulfite rinses to remove excess stain.
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Assistant Professor/Edu
Tasha,
Are you using the PolySci kit k037? Did they change the formulation of the
Bouins? They do make one that has no picric acid, and doesn't last long. If
so, add a some saturated picric acid to the Bouins to see if that helps.
Are you using the Bouins again after microwaving? If so, that
Beth,
We sure could have used the actual article in a lab I know of. The person with
the highest authority, removed them from a lab, and did not want to listen to
what the supervisor had to say. Without the actual article, nothing could
change her mind.
It is common to have spider plants, and
You could boil molds in soapy water, cool, remove paraffin, rinse and dry.
You could place them in the processor on the clean cycle.
You could soak them in xylene, rinse in 100% etoh.
Milestone has a paraffin removal instrument that can be used as well. We just
got one for our student lab and it
We have a few questions to ask:
In your H&E protocols, what is your set up after eosin: 70, 95, 100, 100,
xylene x 3or 95, 100, 100, 100, xylene x 3, or another variation?
What brand of hematoxylin do you use? Eosin?
Do you filter daily or at all? Eosin?
How often do you change the hematoxylin? E
In areas where there are schools, usually there is no shortage. Like here in
Houston, we have 2 programs so no shortage.
In other areas it is challenging to find a tech.
Canada does not seem to have a shortage (that I have heard of), but I know that
it can be hard for areas in the Caribbean to f
Michael,
We just use one of the small desk lamps at a cutting station, the back of the
embedder, and foil to reflect. It works great. Just place the coplin jar with
the slides on top of the control panel of the embedder. Adjust the lamp to the
top of the jar and wrap it in foil. The heat pe
We got some organisms from the microbiology lab and some fresh lung from
autopsy. When the organisms were ready, we 'stuck' the lung with the
inoculating loop and let them incubate for a few days. Then we grossed and
processed the lung. We were given separate specimens for GMs, AFB, and gram.
Well I 'sold' my plasma in the early 1990's. I was in college and needed the
money for gas and such.
When I went to donate my blood, I was suspected of having Hep C, so I stopped
selling my plasma and retested fine.
The instruments looked clean, and there was no debris in the parking lot. But
The Dako is easier and provides better quality. It allows for more user input
and if it goes down, the stains can be completed easily due to the reagent
packs.
The Ventana, in my opinion is for the more novice user. Inexperienced techs,
with a mid level volume can easily operate this unit. It
One technique I use to cut uterus is to face the blocks at room temp, or even a
little warmer. Then I place them on my ice tray and allow them to chill and
soak up a lot of water. Then they are cut last in my set. This allows them to
"cut like butter". My micrometer is set at 3, not 4, and I
Jim,
HT/HTL certification, education level (just because someone has an HT doesn't
mean they have a degree), minimum experience, supervisory or mgt experience,
and intermediate computer knowledge. Graduation of an approved program is not
necessary, if they hold HT/HTL cert. CLIA eligible t
Sandra,
On some holders you can easily convert back and forth from low to high. If
your service person does not know anything about that, I would ask Leica.
Specifically ask for a tech rep with the most microtome experience (in years).
That person should be able to tell you what part you nee
Charles,
The Peloris is great!
The advantages is that it is very user friendly. You can run two different
protocols at the same time, due to the dual retorts. It conserves reagents,
because it calculates based on the number of cassettes you tell it you ran. It
automatically rotates the reag
To help this you could place the cassettes in the other processor in the last
100% for about 5 min, just to freshen them up. Then proceed with the remainder
of the process as usual.
FYI, this will be one of our discussion questions this year in our HTL program.
T
Toysha N. Mayer D.H.Sc., MBA
I have used fabric softener to surface soften nails. It worked ok, but took a
lng time.
Quality was pretty good.
Toysha Mayer
Message: 1
Date: Thu, 13 Apr 2017 11:33:56 -0700
From: Angela Lamberth
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fabric softener to "decalcify" bon
In my opinion a stainer would come first. As it has already been stated,
stainers provide consistency and reliability in the lab. You can always find
someone to assist with coverslipping, but not everyone can perform the stain
just right.
Newer coverslippers are wonderful, they last and work g
I agree with Rene. To discredit the pathologist theory show if all of the
specimens from the run are not fixed properly. Then show if it is just the
LEEPs. Then show if it is that particular clients specimens? Then onto that
client's LEEPs.
That should prove your problem lies with the clien
We use chux here as well. The cotton in them can bunch up, also they don't lay
completely flat. We also use the thin lab mats. Cardinal carries them as does
fisher. They can be bought in bulk, have an absorbent side and a fluid
resistant side. Since we are on a tight budget here, and studen
Pam,
I worked with our microbiology department and had them to obtain some positive
organisms and with our surgery and morgue for fresh tissue. We then inoculate
the tissue and process it. Positive blocks are kept on hand and used. We also
pass a copy of the slides back to the microbiology de
Stephen,
This question is being asked all over the country. Your suggestion of having a
clear cut career ladder is good. Add to that the opportunity to cross train in
other areas such as POCT testing, molecular, and digital imaging. Today's
techs want opportunities to advance and most of them
Try DR Instruments for a decently priced knife, less than $10 each. They have
the older thick, metal handled scalpel that we get for our students. They dull
pretty quickly and are easy to keep up with. The thin ones can hurt your
hands. They sell to the public, and I just googled them.
Other
Lisa,
I have used all of the previously mentioned methods for cleaning molds. It
really depends on the facilities that you have access to. Boiling in hot soapy
water is great for deep cleaning, but you may not have enough pots or beakers
to clean them all at once. Xylene, followed by 100% is
Kathleen,
I would suggest you contact one of the Veterinary Schools for assistance. I
remember that we had some when I was at LSU Vet School, but not much else.
Also try one of the Marine Biology programs down in Florida, they may be able
to help.
Sincerely,
Toysha N. Mayer, D.H.Sc., MBA, HT
Lester,
For safety's sake, as well as laymen peace of mind, I would place them in the
normal tissue disposal biohazard boxes. Notify the proper areas as to what
they are so that they can be aware. Most other areas in the hospital will feel
better, but the number crunchers may be upset because
This is very interesting. I have worked in the field for over 20 yrs, and was
pregnant while working in a small lab. Mostly everything was manual, and I did
not use all of the safety precautions I should have (my fault). My son had
severe speech and language delay as well as a language process
I have used the older Dako Artisan and loved it. It is easy to adapt to you
lab protocols and if it goes down, you can easily pick up from where you left
off. The customer service was great (it has been a while since I had an
instrument), and replacement kits and labels were easy to get. The
38 matches
Mail list logo
