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Hi Everyone,
I'm curious about different PMS2 antibodies and staining protocols for the
Leica Bond III Autostainer. I'd appreciate any feedback from individuals
performing this stain on this platform, including antibody clones and staining
protocols.
Thanks in advance!
Roger
Hi Everyone,
I'm curious about what time everyone stops accessioning specimens for the day.
We receive specimens pretty late in the day, and we're evaluating our
accessioning cut-off times to improve the TAT of these late specimens. We're
focused more on biopsies, but we're also curious about
, Meadowbrook, PA
St. Luke's Hospital, Duluth, MN
PRHS, Naples, FL
Roger Heyna rhe...@lumc.edu 12/9/2013 2:47 PM
I am putting together a list of facilities that have discontinued the use of
negative reagent controls for IHC. After the CAP revised the requirement
related to negative controls when polymer
I am putting together a list of facilities that have discontinued the use of
negative reagent controls for IHC. After the CAP revised the requirement
related to negative controls when polymer-based detection systems are used, we
decided to investigate whether discontinuing the negative controls
that the drying times can be
identified?
I think that you should tally this artifact frequency and intensity
against months of the year and referring dermatologists. You may even
end with enough information to write something about this artifact after
all.
René J.
From: Roger Heyna rhe...@lumc.edu
We are experiencing a staining artefact on our HE's, most commonly seen on
skin specimens, but also observed on other specimen types as well.
Within the dermis and subcutaneous regions of the skin specimens, there are
portions of what should be collagen that appear homogenized and stained
run, or something like a rapid run?
On Monday, August 19, 2013, Roger Heyna wrote:
We are experiencing a staining artefact on our HE's, most commonly seen on
skin specimens, but also observed on other specimen types as well.
Within the dermis and subcutaneous regions of the skin specimens
Hi Histonetters:
When a pathologist identifies extraneous tissue on a slide, and histology
determines that the tissue contamination originates from embedding, what is
your procedure for correcting the embedding? Do you remove the extraneous (or
contamination) tissue from the block, recut the
We have been using tissues from an old autopsy case as our adenovirus IHC
controls, and we are nearly out. I am not able to find another adenovirus case
within our institution that we can use for controls.
Does anyone know a vendor that supplies these controls? Also, is there a
reference lab
We use Ventana's HP antibody, and we do see some of the small, non-specific
globs of stain, but the pathologists are comfortable differentiating between
the true and false staining since they are familiar with this artefact.
Roger Heyna
Rae Staskiewicz raest...@grics.net 5/7/2013 12:56 PM
there are no
quality issues on a given day?
We operate in Sunquest CoPath.
Thank you,
Roger Heyna
Histology Supervisor
Maywood, IL
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stain
using an antibody cocktail. I still don't think we're doing it correctly,
because we're billing for three, but at least it sounds like we can bill for
two, which seems logical.
Have others heard something different?
Roger Heyna
Maywood, IL
Bitting, Angela K. akbitt...@geisinger.edu 3
Our lab currently bills for three, and I know of other labs in our area that
also bill for three. I have heard rumors that this may not be allowed in the
near future.
Roger Heyna
Maywood, IL
Debbie Granato debgran...@yahoo.com 3/15/2013 11:00 AM
We have a billing question for the PIN4
Our lab would like to routinely run the Ubiquitin IHC stain on formalin-fixed,
paraffin-embedded brain and liver(mallory bodies), and I was wondering if
anyone could recommend a primary antibody. We are currently using the Ventana
Benchmark XT autostainer.
Thank you,
Roger Heyna
Maywood, IL
minute antibody incubation
A/B Block
I hope this helps!
Roger Heyna
Maywood, IL
Zimmerman, Billie bzimm...@gru.edu 2/22/2013 3:20 PM
Does anyone perform this antibody?? We can't get it to work on the Ventana
Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small
size
and managing the cover tiles?
Are the three black slide drawers durable?
Thank you,
Roger Heyna
Maywood, IL
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carbonate.
Roger Heyna
Maywood, IL
Johnson, Kevin kjohn...@med.miami.edu 2/8/2013 12:00 PM
Dear all,
During an overnight tissue processing cycle, a malfunction occurred such that
the sample basket was suspended in mid-air for several hours at probably the
worst spot in which to do so
Cox, HTL/SCT(ASCP)QIHC
Message: 7
Date: Wed, 06 Feb 2013 13:15:35 -0600
From: Roger Heyna rhe...@lumc.edu
Subject: [Histonet] Leica Bond III Opinions
To: histonet@lists.utsouthwestern.edu
Message-ID: 51125777022300049...@gwgwia1.luhs.org
Content-Type: text/plain; charset=us-ascii
Could I get
on the Bond III?
Are there antibodies that you were not able to run on the Bond III?
Is the machine reliable, or does it break down often, requiring technical
service?
How arduous is cleaning and managing the cover tiles?
Are the three black slide drawers durable?
Thank you,
Roger Heyna
Maywood
We've seen this problem before in our lab, and it was traced back to the mold
release, just as Sheila said.
Roger Heyna, BS, HTL
Loyola University Medical Center
HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca 1/29/2013 5:08
PM
Are you using mold release? Sometimes that can cause
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