Re: [Histonet] Tissue falling off slides

Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks that I cut and stain

Re: [Histonet] polyomavirus antigen immunostain in urine cytology specimens

Yes, what would you like to know? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax

Re: [Histonet] Shipping Slides

We put our send-out slides in the 5 slide plastic holders then place them in Jiffylite Sealed Air cushioned mailers (there are different sizes available). If we are sending out a large quantity of slides we will use FedEx's Padded Pak, but it will cost more than a FedEx envelope. Richard

[Histonet] Question - OR specimens to Pathology

How are other hospitals transporting fresh tissue specimens from operating rooms to the Pathology Frozen Section room for an intraoperative consultation? Occasionally, we get specimens delivered on towels and I don't think this is appropriate. I think they should be in a container that can be

Re: [Histonet] 88363 cpt code

Is there a Technical charge (TC) for 88369 or is it only a Professional charge that the pathologist bills? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

Re: [Histonet] DAB enhancer

I don't know why anyone would want to use a DAB enhancer for slides prepared on Leica-Microsystems' Bond Max platforms. The resulting immunoreactivity is exceptionally Robust in my experience when using their Bond Polymer Refine Detection kit. Could this request be for a protein target

[Histonet] HHV-8

Is anyone doing PCR for HHV-8 on formalin-fixed, paraffin-embedded tissue? We have a very interesting autopsy case where we would like to test brain tissue. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

Re: [Histonet] Adenovirus Tissue Controls Needed

I do IHC for Adenovirus in my laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax

Re: [Histonet] p16 on the Bond

We dilute the CINtec p16 RTU antibody (Ventana/Roche) 1:10 (15' primary antibody incubation) and use high pH retrieval (H2) for 10' on the Bond Max. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] Re: [IHCRG] p40 staining for breast myoepithelial cells

We have not validated it for this purpose, but it does label myoepithelial cells in breast tissue very nicely. FYI - We are using BioCare's RTU p40 on Leica's Bond Max. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

[Histonet] Question - MDM

Is anyone using a commercially available antibody (not RUO) for the immunohistochemical detection of MDM in liposarcoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

[Histonet] Re: [IHCRG] p40

I have been very impressed with BioCare's p40 monoclonal predilute. We are able to use it a 1:5 dilution on our Bond Max platform. Excellent immunoreactivity so far! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant

Re: [Histonet] IHC (DAB) after HE?

No. Re-hydrating the tissue will remove most (if not all) of the eosin and the hematoxylin will serve as your counterstain (although you may need to counterstain again at the end of you protocol). If you use antigen retrieval for an unstained slide, you will have to use it on the HE-stained

Re: [Histonet] FW: Primera Printer

Can this printer be interfaced with CoPath? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax

Re: [Histonet] Glutamine Synthetase and HSP 70 IHC test availability

We are doing these markers, but, to be perfectly honest, I am not very impressed with their results. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street

Re: [Histonet] Antibody IDH1

We run it on Leica's Bond Max with good results. We use the mouse mAb (Clone H09) from dianova at a dilution of 1:50 (15' incubation) following low-pH retrieval. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant

[Histonet] Help!

I need to speak with someone who has experience with doing (and interpretating) in situ hybridization for EBER. Please contact me directly. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

Re: [Histonet] Process and hold?

I'm surprised that laboratories are not validating extended fixation times for their breast specimens rather than jumping through all these hoops. There are now several published articles demonstrating no reduction in immunoreactivity for ER, PR, and HER2 in breast specimens kept in formalin

Re: [Histonet] P16 on Leica Bond

When we obtained the p16 antibody from mtm Laboratories we used it a dilution of 1:10 on the Bond Max. I hope that the concentration will not change now that Ventana is selling it. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Assistant Director, Anatomic Pathology

RE: [Histonet] Help with CPT code 88363 for archived tissue retrieval

I'm not 100% sure, but I don't think this CPT code has a Technical component. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860)

Re: [Histonet] Helicobater Pylori QC block and/or slides

I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard

[Histonet] Argene Inc.

Does anyone know if Argene/Biosoft (France) still exists and, if so, do they still sell products here in the USA? They had a number of interesting infectious disease monoclonal antibodies. Thank you! Best wishes to everyone for 2013! Richard Richard W. Cartun, MS, PhD Director, Histology

Re: [Histonet] CAP and negative IHC controls

That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a Negative Reagent Control for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent

[Histonet] PAI-1

Does anyone do IHC for PAI-1? Thank you. Happy Holidays to all and best wishes for 2013. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT

Re: [Histonet] Renal biopsies and muscle biopsies

I have been involved in the immediate adequacy assessment and triage of renal biopsies for a very long time here at Hartford Hospital. We now have a team of two pathologists and myself that are called to interventional radiology and our children's hospital for renal biopsy procedures. I also

RE: [Histonet] Stain for HP

For labs that are staining ALL gastric biopsies, Are you charging patients for this when there is no inflammation present?. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital

[Histonet] Fwd: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

FYI Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax ___

Re: [Histonet] Breast processing

My personal experience is that formalin fixation for 72 hours (and longer) does not affect immunoreactivity for ER, PR, and HER2 protein overexpression as long as the tissue does not dry-out before being placed in formalin and thin (2-3 mm) sections are submitted for fixation and histologic

[Histonet] RE: Negative Controls

The Evidence should come from your own laboratory. Just because we don't run Negative reagent controls in my laboratory doesn't mean it's appropriate for you to discontinue their use for your laboratory. Your laboratory Medical Director needs to make this decision based on what he/she sees

[Histonet] RE: Antibody validation

There is no one right answer for the number of cases needed to validate an antibody for diagnostic immunohistochemistry. That decision must be made by the laboratory's medical director, not an outside organization. Many of the primary antibodies available today have proven track records and

[Histonet] Question

I was asked by a colleague if there is testing for fibrinogen storage disease that can be performed on fixed tissue. I told her that I did not know, but I would ask the experts. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen

Re: [Histonet] PAN CKMNF116, picking up dendritic cells?

It should label dendritic cells since it reacts with cytokeratin 8. These cells have been shown to contain cytokeratin protein by immunoblotting. I use this reactivity as an internal positive control. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director,

Re: [Histonet] charge code for send out's?

We now request a FedEx or UPS account number for all outside requests for pathology materials from our Department. It is the responsibility of the requesting hospital/laboratory/company to provide this since they will be providing a consultation, performing IHC/molecular testing, or conducting

Re: [Histonet] charge code for send out's?

Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Richard Cartun rcar...@harthosp.org 9/9/2012 10:16 AM We now request a FedEx or UPS account number for all outside requests for pathology materials from our

[Histonet] Question regarding retrospective testing

I know that this had been discussed before, but I believe regulations have changed and I need to find out what other laboratories are currently doing when it comes to retrospective testing (histochemical stains, IHC, molecular testing, etc.) on cytology and surgical pathology specimens that

Re: [Histonet] p16

In order to troubleshoot, one would need to know the following: Test tissue Fixation Antibody clone, dilution, and incubation time Detection system Antigen retrieval Positive control tissue Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection

[Histonet] Negative Reagent Control

Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last

Re: [Histonet] congenital syphilis

Immunohistochemical testing for Treponema pallidum is the preferred method of identification. There are labs (including my own) that offer this. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] GBS IHC

Does anyone know of a good antibody to Group B Streptococci (GBS) that works on formalin-fixed, paraffin embedded tissue? I had a wonderful mAb from a company in Canada, but they went out of business. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology

Re: [Histonet] Renal Core Biopsy

Are you referring to biopsies done for medical renal disease or tumor? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596

[Histonet] Lab Office position

I know someone here in Connecticut who is thinking about re-locating to the Melbourne/Palm Bay area of Florida. She currently works in a busy Anatomic Pathology office in a hospital doing specimen accessioning, consult accessioning, slide(s) send-outs, slide filing, etc. Are there any jobs in

[Histonet] IHC for p21, p27, and RB

What has been people's experience with immunohistochemical staining for p21, p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection

Re: [Histonet] Antibody titer

Yes, I always want to see the immunoreactivity drop-off (or even disappear) with a higher dilution(s). Otherwise, you might be wasting antibody by using a dilution that is too concentrated. Also, be careful with the recommended dilution. That dilution is only applicable if you are using the

[Histonet] Old cryostat for parts

We have an old Miles Tissue-Tek II cryostat that is going to be discarded unless someone would like it for parts. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

Re: [Histonet] cryptosopridia

Yes, most testing is performed in the Microbiology Laboratory; however, I have an IHC test for formalin-fixed, paraffin-embedded tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

[Histonet] Who pays ......

for the express shipping when another medical institution requests a patient's pathology slides and/or unstains or paraffin block for additional testing? In my opinion, the requesting institution should provide a FedEx, UPS or other account number for this. After all, they will be billing the

[Histonet] Biospecimen collection

This inquiry applies to those of you collecting human tissue for research. I am being asked to develop pricing for the following activities regarding collection of biospecimens from surgery, image-guided biopsies, endoscopic biopsies, bone marrow biopsies, and body fluids: 1. Collection of

Re: [Histonet] Leica Peloris Processor

In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital

Re: [Histonet] Batch Controls

As the laboratory Director, I can sign-off on the positive control slides or my staff can do it since I have delegated this responsibility to them. We all use a different color pen to mark the slide(s) O.K. so we always know who signed-off on the control slide. All positive controls are filed

[Histonet] know error system - prostate biopsies

I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the know error system for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

Re: [Histonet] Aetna and In-Office Lab Accreditation

This was released today. Richard Statline Special Alert: New Evidence Links Self-Referral Labs to Increased Utilization, Lower Cancer Detection Rates Study co-funded by CAP Appears in April 2012 Issue of Health Affairs April 9—Self-referring urologists billed Medicare for nearly 75% more

Re: [Histonet] AFB by IHC

Yes, you can do IHC for mycobacteria; however, there are issues with specificity depending on the primary antibody that you use. I have been using a polyclonal antibody from Dako (no longer available) for many years and although it is very sensitive for identifying mycobacteria, it does

[Histonet] IHC for interferon gamma

Has anyone succeeded in doing IHC for interferon gamma on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

Re: [Histonet] Fixatives for breast

1.) Formalin, 2.) Formalin, and 3.) Formalin. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax

Re: [Histonet] IHC + control question

I know that many labs run Vimentin for this, but please give consideration to running a nuclear marker as well especially since some of our predictive markers (ER and PR) are nuclear proteins. In my opinion, Vimentin is very robust and may show-up even when the tissue is not adequately fixed

Re: [Histonet] Cytokeratin AE1/AE3

It may be specific labeling of extra-follicular dendritic cells that contain low molecular weight cytokeratin and, if your IHC assay is truly optimized, you won't be able to get rid of it. I use the LMW CK mAb clone 5D3 that labels CK8/18 and I see this reactivity all the time. I like it

[Histonet] mAb to Serotonin

Does anyone have a good monoclonal antibody to Serotonin that they would recommend for diagnostic immunohistochemistry performed on formalin-fixed, paraffin-embedded human tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen

Re: [Histonet] (no subject)

Complete loss. You can see patchy immunoreactivity in tumors with these markers. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102

[Histonet] Brucellosis

I have a consult (transplant kidney biopsy) that I believe is infected with Brucella. My IHC test is positive, but I have never seen a human case before. Can someone familiar with this disease forward me a photo or a good reference that shows the typical morphology of this organism in human

Re: [Histonet] H. pylori

Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen

[Histonet] Question - Pancreatic CA Research

We have a patient with pancreatic cancer that is close to death. The patient has requested that her body be donated for pancreatic cancer research. Is there anyone in the Histonet Community that is working in pancreatic cancer research that can help me with this patient's request? Thank you.

RE: [Histonet] RAC Medicare Audits - PAs

] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Tuesday, January 31, 2012 7:19 PM To: Histonet Subject: [Histonet] RAC Medicare Audits - PAs Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents

[Histonet] RAC Medicare Audits - PAs

Is anyone familiar with the new requirement effective January 1st, 2012 that states that Pathologists' Assistants can no longer teach residents unless a pathologist is present? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

Re: [Histonet] clostridium and bacterial spores in tissue sections

Hi Liz: I have used IHC to identify Clostridia on formalin-fixed tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860)

Re: [Histonet] new anitbody

You need to prove that the antibody labels its intended target and that there is no cross-reactivity with unrelated proteins. The number of cases needed to do this will depend on the antibody's track record and its purpose. For example, antibodies used to identify predictive targets (ER, PR,

[Histonet] Antibody to Cryptococcus

Unfortunately, we have used the last micro-liter of our Cryptococcus antibody that we obtained from DAKO years ago. Since DAKO no longer sells this antibody I am wondering if anyone is using another commercially-available antibody to label Cryptococcus in formalin-fixed tissue with

Re: [Histonet] control block log

I use FileMaker Pro software to keep track of our IHC controls. I created a control tissue file that includes the following: Control #, Case #, Tissue site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks submitted, and then a Comment field for the immunoreactivity for

[Histonet] IF on lung tissue

Does anyone still do direct immunofluorescence on frozen lung tissue looking for immune-complexes, and do you find it helpful clinically? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] Question - CPT Coding 88321

I'm confused (what's new!). I'm being told there is no technical component (TC) for 88321 (slide consultation). Is that correct? There has to be a technical component to cover accessioning. Thanks for your help. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology

[Histonet] Pathologists' Assistants - Survey

I need your help. This question is for Anatomic Pathology Laboratories located in hospitals where the pathologists are in private practice. Do you employ Pathologists' Assistants in your Anatomic Pathology Laboratory? If so, are they employed by the hospital or do the pathologists employ

Re: [Histonet] release of tissues back to patient

In the state of Connecticut, the only agency licensed to handle human tissue or remains (other than hospitals) is a funeral home. Therefore, our policy is to only release tissue or remains to a funeral home after the patient or family has made the appropriate arrangements with them. Richard

RE: [Histonet] SALARY

I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like Free Agency in baseball; if you want a good player, you need to put the money on table. Histotechnologists are the most valuable employees in the

[Histonet] Question - Prostate biopsy reports

How many of you who are reading prostate biopsy specimens in a hospital laboratory incorporate a diagram of the prostate in your report that shows the anatomical location of benign, PIN, and adenocarcinoma pathology in each quadrant? Thanks. Richard Richard W. Cartun, MS, PhD Director,

Re: [Histonet] Slide/Block Retention

We currently keep slides and paraffin blocks (surgical pathology) for 17 years; I would keep more, but laboratory administration keeps tell me to discard because we don't have sufficient storage space. It's not unusual for me to receive a request for a block(s) from the 1990's for IHC,

Re: [Histonet] Block age/labile epitopes - UPDATE

An interesting article on this subject came out earlier this year from Stephen Hewitt's work group at the NCI. Their work shows that the loss of antigenicity in archival formalin-fixed, paraffin-embedded tissue sections is due to the presence of water, both endogenously (poor processing) and

[Histonet] IHC for TSPY

Does anyone offer immunohistochemical testing for the Y-encoded TSPY protein in testicular biopsy tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80

[Histonet] Antibody to Cryptococcus

For years (maybe decades) I have used Dako's rabbit polyclonal antibody to Cryptococcus for IHC staining of human tissue. The antibody is no longer available and our last remaining vial is almost empty. Can anyone recommend another antibody that works on formalin-fixed tissue? Thank you.

[Histonet] Immunotech

Does anyone know if Immunotech (France) is still around and, if so, do they have a distributor here in the US? We obtained an antibody to CD1a (clone O10) from them years ago through a distributor in Maine whose telephone number is no longer active. Thank you. Richard Richard W. Cartun, MS,

Re: [Histonet] cam 5.2

I have used CK-CAM5.2 for years with excellent results. However, for the past several years I have been using Leica-Microsystems' low molecular weight CK antibody, clone 5D3, that labels cytokeratins 8 and 18. I highly recommend it. Richard Richard W. Cartun, MS, PhD Director, Histology

Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections

Re: [Histonet] IHC for H. pylori

We only run IHC for H. pylori when the appropriate inflammatory background is present or when the patient has tested positive in the past and we receive a follow-up gastric specimen where bugs are not identified on HE. Running H. pylori IHC on every gastric biopsy is uncalled for and borders on

[Histonet] Question - Anatomic Pathology Manager

How many Hospital-based Pathology Departments with 55,000 surgical specimens, 100,000 PAPs, and 6,000 non-GYN cytology cases have separate managers for Cytology and Histology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection

[Histonet] Dpc4 IHC

Is anyone doing IHC for the Dpc4 gene product for adenocarcinoma in biopsy specimens of the pancreas and bile duct and, if so, do you find it useful? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant

[Histonet] HCV testing

Is anyone doing PCR testing for Hepatitis C Virus on formalin-fixed, paraffin-embedded tissues? If so, can you find the virus in tissues other than liver? Maybe in blood within vessels? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen

Re: [Histonet] question on H pylori

We use IHC on cases that show the appropriate inflammatory background. Please note that histochemical stains may not identify intracellular H. pylori. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] Submission of tonsils to pathology

What is your policy on the submission of tonsils to pathology for examination (gross or microscopic)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

[Histonet] Submission of tonsils to Pathology - #2

I apologize; I should have stated my question differently. Our Children's Hospital wants to stop sending tonsils to Pathology unless the surgeon requests pathological examination. An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.) concluded that pathologic

[Histonet] Re: Her2 CAP survey

Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic

Re: [Histonet] Her/2

Yes, our slides look weak. I did slides for another hospital and they look weak as well. I have already contacted the CAP to see if they have received any other complaints. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

Re: [Histonet] IHC validation

In my opinion, only if it affects immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860)

Re: [Histonet] Controls for IPs

This response is late due to the overwhelming time commitments of NCAA Basketball Tournaments. The only multi-tissue control we use is for HER2 protein overexpression. In my opinion, multi-tissue controls are completely unnecessary for every-day use for the majority of IHC tests that we do.

[Histonet] IDH1 R132H IHC

Is anyone doing IHC for the IDH1 R132H point mutation for astrocytoma and oligodendroglioma in formalin-fixed, paraffin-embedded brain tissue using the H09 clone? If so, how do you get the antibody? Directly from Germany or is there someone here in the USA that carries it? Thank you.

Re: [Histonet] use of destained sections in IHC?

Yes, you can use HE-stained slides for IHC; however, if your lesion/tumor is negative and there is no internal positive control, then you may be dealing with a false negative result. Having said that, we have had good luck with the majority of proteins we have tested. Richard Richard W.

Re: [Histonet] CAP question ANP.22970

Here's an excellent reference - Ross JS: Saving Lives with Accurate HER2 Testing Am J Clin Pathol 2010;134:183-184. I quote, A number of experts in the field have now agreed that a laboratory performing HER2 testing in the US patient population should have a HER2+ rate of approximately 16%

RE: [Histonet] CPT code 88363

not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent

[Histonet] CPT code 88363

Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection

[Histonet] Consult accessioning question

When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new Date-of-service? If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday? Thank you.

[Histonet] C3d

Is anyone using a Commercially-available C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant

Re: [Histonet] RE: decalcifying bone marrows after processing

Hi Becky: How long is the specimen in formalin before you put it in Cal Rite decal? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596

Re: [Histonet] Need help with p16 pricing

We use the mtm predilute mAb with excellent results. Due to the sensitivity of our detection (Leica-Microsystems' Bond Polymer Refine kit), we are able to dilute it to 1:10 and still obtain outstanding immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology

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