Hello everyone, hope this Monday is treating you well. I am in dire need of
some assistance. We are a dermpath lab in Knoxville, TN using the Leica Bond
for our IHC. We have been unable to acquire our Refine Red Detection Kits due
to some technical difficulty. I am looking for anyone who curre
Good morning histonetters,
Is there anyone out there who is currently doing ISH HPV probes on the Leica
Bond? If so, where are you getting your tissue for positive controls? Any
help would be greatly appreciated. Thanks
SheilaKnoxville, TN
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Histo
Hello Histonetters,
I need your help. I have a doc looking for someone to perform an IHC stain for
us. It's a p41/38 moab antibody for HHV-6. If anyone knows of someone doing
this stain who also accepts outside cases, I would really appreciate the
contact info.
Thank you and have a wonderful Mo
Good afternoon Histonetters!
Does anyone out there know of a good antibody for Bartonella? I would
appreciate any information you could give me. Thank you for your knowledge and
willingness to share information.
SheilaKnoxville, TN
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Hi Claire,
I may be grasping at straws here, but I went to an NSH workshop once where this
very question was posed. You won't believe what the cause was. They had a bad
lot of blades. The whole lot was nicked, so changing to a new knife or a new
box of knives did not help the problem. Chan
Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond. I am
using a Biocare primary antibody. I've tried ER1 for 20 min. with 15 min. Ab
with no luck. Does anyone do this, and do you think increasing the primary Ab
time would help? I've used this same Ab before o
Good morning Histonetters,
Can anyone tell me if you are using a particular company's reagents for your
H&E staining that are pre-made and meant to work together? Also, is the cost
significantly more than buying separate reagents and making up your own?
Thanks,
Sheila, HT (ASCP)
Knoxville, T
? You use regular superfrost plus slides for temperature verification of the
thermal pads? The slides to use are the superfrost plus slides from Ventana
that have a qc'd strip on them that will turn black if the temperatures of
pads are at acceptable levels. How do you prove that with a plain
sup
Sally,
This is a little different from what I understood yesterday. I thought that
it was stated you could only bill once per part, such as an S-100 on part
A,B,C would be fine, but not on A1,A3, and A5.
I did not read anything about only being allowed to order one antibody. I
thought panels wer
Happy Holidays Histoland,
Does anyone out there have a good working protocol on the Ventana Ultra for
MiTF. I have tried several but the docs still aren't happy with it. Could
you share any info you may have regarding vendor, system, protocol, etc.?
Much thanks!
Sheila, HT (ASCP)
KDL
Would the person asking about a used Biocare Decloaker please contact me.
We have one that is like new. No longer use it since all retrieval is online
with automated stainer.
Would be willing to sell.
Thanks,
Sheila
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His
WELL SAID!!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Wednesday, December 07, 2011 5:47 PM
To: Rene J Buesa; joelle weaver; histonet@lists.utsouthwestern.edu; Nicole
Tatum
Subject:
Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath
specimens, and who is your vendor? Do you like the products, and what
platform are you using?
Thanks for any information you may have. These are new requests from our
pathologist.
Sheila
__
Histonetters,
Is anyone out there using the EBER ISH probes from Ventana on the Ultra
Platform? I have a few questions. Did you need to get new software
installed? Was there any training involved? And do you have successful
protocols that you are willing to share?
I have asked the quest
Sandy,
I don't think it is a "standard" procedure. However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted. We are a
dermatopathology lab. When it is requested, we cut the whole case
12:16 PM
To: Sheila Fonner
Subject: RE: [Histonet] IHC H.P. Negative Control
Sheila,
Cap requires a negative with each block of patient tissue being stained for
immunos, However they also state that if more than one block from the same
patient is being tested that only one of the blocks is
CAP guidelines clearly state that you must run a negative control for each
patient tissue. However, if you are running a large amount of a particular
antibody, you may validate your procedure to run only one positive control.
However, it is just as easy to run a positive control for each patie
I know that I have requested this information once before but am in need of
it again. Does anyone know of a company that tests for Rocky Mt Spotted
Fever? We are having a hard time finding someone that still carries an
antibody that tests for this.
Any and all info greatly appreciated.
S
Can anyone tell me what is a good Ab to use for staining myeloblasts in bone
marrow specimens? I would appreciate any input.
Thanks
Sheila
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listin
: Sheila Fonner
Subject: Re: [Histonet] Frozen Tissue Protocol
You are seeing endogenous phosphatase, not endogenous peroxidase. Use 10%
acetic acid for 1-2 minutes.
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513
Hi everyone,
Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster. I appreciate everyone's help. Now I am back for more info. I
deleted the depar. and cell conditioning steps and I got specific staining
with little background. There was just one problem.the p
Jan,
Thanks, I really appreciate the help. This is my first attempt, so we'll
see how it turns out. :)
Sheila
-Original Message-
From: Jan Shivers [mailto:shive...@umn.edu]
Sent: Tuesday, August 16, 2011 9:21 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
S
lto:r...@wchosp.org]
Sent: Tuesday, August 16, 2011 7:54 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue
Sheila,
The cell conditioning step is for retrieval. It will depend on what antibody
you are going to run. If you use it for Depar. you will also use it
wiit
Does anyone else leave out the cell conditioning step?
-Original Message-
From: Yahoo [mailto:kim.tourn...@yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
I would leave out the pretreatment step as well i.e. Enzyme, CC1
Thank you.
-Original Message-
From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org]
Sent: Tuesday, August 16, 2011 7:02 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue
You are correct, leave out the depar. step.
Brett Mierow, HT (ASCP)
Histology
Good morning everyone,
I was wondering if anyone could help me with a protocol change for the
Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, and
Zoster Virus on frozen tissue that was received for IF studies. I know it's
not Monday, but it is early, and I was hoping for
Gayle,
We just use small disposable weigh boats to catch our drippings and toss
them when full.
Sheila
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Monday, August 08, 2011 10:56 AM
T
Since I haven't seen anyone talk about this yet, I feel obligated to tell
you all that there is a handy dandy little "melter" out there for getting
the excess paraffin off the blocks.
You just run the edges of the block over it and voila...trimmed.
When you have up to 600 blocks a day, this thing
You sure can't beat an old AO!
The old Leitz microtomes with the wheel on the front were awesome too.
Ahhh memories.
Sheila HT, ASCP
KDL Pathology
Knoxville, TN
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behal
Jo-Ann,
I have seen this problem many times over the years. Nearly 100% of the time
it is due to the level of the alcohol after the eosin being too low.
Whether you water rinse or go straight into a 95% etoh, the level of your
first alcohol after eosin MUST be covering your entire slide. If not,
and could you give me any advice?
All help would be greatly appreciated. Can I make a 50/50 mixture of the
RTU antibodies and use my longest protocol? We are using the Ventana Ultra.
Thank you so much.
Sheila Fonner, HT (ASCP)
KDL Pathology
Knoxville, TN
We get ours from Ventana.
Sheila Fonner, HT (ASCP)
KDL Pathology
Knoxville, TN
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne
Sent: Monday, May 23, 2011 1:46 PM
To: 'Joanne Clark'
Hi everyone,
One of our pathologists is wanting to know if anyone has an antibody they
are using for rickettsia (rocky mountain spotted fever). We used to send
this out but our supplier no longer offers it and did not know of anyone
else doing it.
I would greatly appreciate any information
SAME HERE!!! I would love some help with this. We are going to start doing
the EBER with Ventana and have no idea where to start.
Thanks in advance for ANY suggestions or help you could give.
Sheila, HT (ASCP)
KDL Pathology
Knoxville, TN
From: ih...@googlegroups.com [mailto:ih..
stick to your labcoat sleeve, your
hand, almost anything that passes over that open cassette. Try to find the
source of your problem and you will be able to fix it!
Regards,
Sheila
-Original Message-
From: Bouchal, Rena L [mailto:bouch...@wvuhealthcare.com]
Sent: Thursday, March 17, 201
You can use any cassette you like if you put a blue sponge inside of it.
Some people use two sponges, one on top and one on bottom, or you can use
just one and fold it in half. Another thing you might want to try are the
small biopsy bags. They are like tiny tea bags and you just pour the
content
Hello everyone,
I have an issue that I would like some help with. We are currently running
SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the
regulations being so strict regarding the processing times of breast
specimens, how are you all dealing with that when it comes t
Amanda,
You will lose antigenicity on the spirochete control slides after that long
of a period. We usually cut no more than 10 in advance, and we keep them in
the refrigerator. Hope this helps.
Sheila HT (ASCP)
Knoxville Dermatopathology Lab
Knoxville, TN
-Original Message-
From: his
I am really interested in this also. And in addition to the Simplex I and
II, does anyone know of a good antibody for Zoster (Varicella)?
Thanks,
Sheila Fonner HT (ASCP)
KDL IHC
Knoxville, TN
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
.
Didn't mean to offend anyone. I REALLY was just trying to help and give my
opinion based on my own personal use.
Sheila
From: Victoria Baker [mailto:bakevicto...@gmail.com]
Sent: Friday, February 25, 2011 10:18 AM
To: Greg Dobbin
Cc: Sheila Fonner; histonet@lists.utsouthwester
, February 25, 2011 9:31 AM
To: Sheila Fonner; histonet@lists.utsouthwestern.edu; 'Cynthia Pyse'
Subject: RE: [Histonet] IHC Equipment
The Bond does do ISH. In fact it utilizes the same detection kit so you have
only to buy the probes, not the additional detection kit that could
(dependi
Cindy,
We used to use the Dako stainer, and we still have it as a back-up if
necessary, but we have recently (in the last year) bought a Ventana Ultra.
You can put 30 slides at a time on it, but you do not have to batch the
slides. It is a continuous feed machine, which means that as soon as a
sl
Liz,
You can perform IgG and IgM on FFPE tissue samples. We do them through IHC
on our Ventana Ultra and get the antibodies from Cell Marque. Our
pathologists are happy with them.
Sheila
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.
I would just like to thank everyone for all of your comments regarding the
high complexity testing issue. I obviously opened a can of worms on that
one! I think I understand it a little better now as I have come to the
following conclusion: Although CAP requires strict guidelines and
documentati
Hello All,
I would really appreciate it if anyone has information on whether IHC/ISH
are considered high complexity testing for histotechs. Our pathologist
believes that ALL histology low complexity testing since a "machine" is
doing the work. Can anyone help me out with some guidelines, lite
Does anyone have a current protocol for Factor X111a on the Ventana Ultra
that is working for you? I would appreciate any info or support you could
give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks!
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