Tissue that has been properly processed, cut thin and dried well should not be
falling off.. We have never needed any additive in our water baths but if we
have tough bones or very bloody tissue we use plus slides. PS: we soak tough
or bloody tissue in soapy water with some ammonia as it REALLY
When you open nylon bags they sometime "pop" the biopsy out and you can lose
it. Tiny tissue gets stuck in the the seams of the bags. Blue sponges made
artifact indents in tissue. We use round Obex papers and have never lost a
biopsy. They are wonderful! You can fold them like filter paper ,us
Also heard" Histotechs are good embed!!!" LOL
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histone
For me 36years..will not be 37, I retire in 3 weeks! Off to visit my son
and family in England!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Friday, August 08, 2014 11:01 AM
To:
Over the years we saved interesting gallstones, all shapes and sizes...when it
came time for the head Doc to retire we glued them on a black background board
in an interesting swirl.. had it framed on a really nice shadowbox.. It looked
beautiful.. Weird but beautiful!
-Original Message
Leica 2125
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome
Leica all the
Amen!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham,
Andrea L - (algranth)
Sent: Wednesday, May 21, 2014 4:23 PM
To: Clements, Mary Ann; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE:
Why do they always want to put us in the basement? We have a lot of the
hospitals explosives and flammables??
Still, as long as they have a good ventilation (you often need a roof or
outside wall to do this) it might be ok. I hope they have good environmental
engineers.
-Original Message---
Our req's come over the computer , not with specimen. We then check the req
against the label on the specimen.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Monday, March 24, 2014 5:20
10 turns of the wheel is fine for most tissue however we often get FNA's that
are so tiny that several turns of the wheel and you will be completely through
it. It is often a judgment call by the tech.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-b
We do 3 levels on all..taking hp immuno on 2nd level of gastrics and a couple
of extra on esophs. ( in case of alcian blues or pas/fungus).
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
imhype...@aol.c
We use the low profile for everything...Accu-Edge. They are superior.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor
Jordan
Sent: Friday, March 07, 2014 4:22 PM
To: histonet@lists.utsouthwest
We like Fisher's Red Control Slide with Adhesion #22-042-910
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, February 28, 2014 9:58 AM
To: histonet@lists.utsouthwestern.edu
Sub
Incredible! Lee Luna (who wrote the book on histology) always said" rehydrate!
rehydrate, rehydrate" Where are these so called supervisors coming from???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of b4
Right, it's the only way to be sure bone is really decaled enough. We also used
to use it to find calcium in lumpectomies. I am sure we were not doing proper
monitoring of it though as it was in our dept. and radiology never checked it.
-Original Message-
From: histonet-boun...@lists.ut
I had a pathologist who said we were all " laboratory scientists", now if the
pay reflected that
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Wednesday, September 11, 2013 9:58 AM
You can shop all the blades in the world( and we have tried many) but if you
care about quality sections the Accu-edges are the only game in town.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Ja
You need to tell your manager that you cannot do your job without proper tools.
Only the tech cutting knows how many blades he or she needs to cut a days work.
These micro managers need to do some bench work and get a reality check.
Unbelievable!
-Original Message-
From: histonet-boun.
We tried them but they have a tendency to warp and you will not get a flat
surface for cutting.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, May 09, 2013 2:04 PM
To: Myer
The problem seems to be that the clamp screw handle is welded on and that is
the weakness. On the old tall Leicas (does anyone remember them?) the screw-in
lever was solid and we never had this problem. Also the springs get rusty
inside the clamps probably from corrosive surface decal. It seems
PS.. Even with the clamp problem I think the Leicas are superior.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth
A (NIH/NIDCR) [E]
Sent: Friday, March 08, 2013 2:01 PM
To: 'Weaver, Stephan
Many vendors are pushing different blades...but the only blade that is superior
is the Accu-edge. If you are using any other you will very likely get inferior
sections. That is 30 years of experience using Accu-edge blades always trying
others and always going back to the best.
-Original Me
We put in a procedure stating that should we change the schedule we will test a
variety of tissue (10 different types) and have the pathologist assess the run.
We have not changed a schedule in years but we seek to comply...
-Original Message-
From: histonet-boun...@lists.utsouthwestern.
I would have to consider the tech who is working continuously as senior. That
is the tech that I would rely on the most.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, Februa
We have the clinical lab check our friges and freezers on the
weekends..everything else is off.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan,
Beatrice
Sent: Friday, February 08, 2013 7:52 AM
T
70,80 95,95,100,100
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PRESZLER,
JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH
Sent: Thursday, February 07, 2013 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject
We've always used 30 minutes and it has never failed.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey
Sent: Thursday, December 06, 2012 8:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Hi
VIP always!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, December 04, 2012 3:57 PM
To: Tim Wheelock; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Processor
We put our molds in the VIP before running the cleaning cycle daily. Then we
dip them in alcohol containing mold release..air dry and store.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Se
Ice is still the best coolant because it adds moisture. As Lee Luna used to
say" Rehydrate, rehydrate, rehydrate" For tough blocks, face it then add soapy
water to ice with some ammonium hydroxide. Like magic!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:hi
We never had a problem until they changed AB's on us. We sent them slides
procedures etc to test but in the mean time we tried Cell Marques AB and it was
clean. Problem solved. Now we all need to demand that Ventana switch back to
Cell Marques H pylori so that we do not have to pay for those #@*
I was also tired of digging bone marrow particles and biopsies out of the
stitching.
Some people like them because they can just dump tissue in them but they do not
have to fight with them when embedding. Biopsy cassettes can trap air and
float. The best all around product is Obex round papers.
Boy, they sure like to put us in the middle of what should be their own
problem. Thank heavens I only work now for one Dr at a time now but when I was
at a larger place they rotated. They still used to tell us to give them
particular cases when it was not their turn so that we got the flak when
Does anyone know a retic stain that does not use uranyl nitrate?
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I read an article once that said spider plants absorb formalin fumes we have
kept them in the histo lab for years.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Friday, May 11, 2012
I'm Frisbeetarian, we believe when you die your soul goes up on the roof and
you can't get it down. :)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
(SCHN)
Sent: Tuesday, March 27, 2012 7:
Overnight processing is usually optimal for average size tissue. Larger tissue
may not get optimal processing and biopsies may be a little over processed. It
is the nature of tissue processing in a general lab that does not have several
processors with different programs. A solution is to rehyd
Workhorse...has all the requirements we need.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Giracello,
Darlene
Sent: Thursday, February 16, 2012 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [H
Time off is one thing but holidays are another. Holidays need to be rotated.
Seniority should apply to things like choice of hours but not holidays.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela B
We usually go by "first asked" days off. (We keep a calendar for this purpose)
But for holidays we rotate. If someone had the day after Thanksgiving off last
year they let the next in line have the choice.( I keep the previous years
calendars for checking) If no one else wants it they can then h
When tissue falls off the slide it is usually poorly cut sections or a problem
with under processing or bad tissue ( bones) GI biopsies should not be falling
off if cut well and processed correctly. Keep an eye on how she is cutting, she
may have a problem with her microtome ( are all screws tig
If you use Clearium mounting media from Surgipath you can coverslip out of
isopropyl alcohol.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Tuesday, September 27, 2011 11:35 PM
We are a small hospital and though we no longer do in house autopsies we still
had an autopsy assistant.(most prefer to be called this) We always had a pool
of people available to do this job. Training as a histotech does not include
this job and I have always refused to do it. I know there are
Would you ask a surgeon to speed up his procedure? We are also using sharp
blades and cutting off the tip of a finger is a real outcome to speed cutting.
What we do effects patient outcome and quality must always come first. Speed
come gradually after. When you treat patient tissue on a assembly
Not spit ,saliva and we leave it on for 15/20 minutes and then rinse well. I
have had techs who did not like doing it so we bought the enzyme and after all
the hassle she found it did not work as well...now we all think about chocolate
and salivate. :)
-Original Message-
From: histonet-
We have always used water with liquid soap and some ammonia to soften blocks.
It has had no effect on IHC.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, December 27, 20
We use about the same amount but in our last 100% Alc.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Thursday, October 21, 2010 4:40 PM
To: Scott, Allison D
Cc:
Subjec
Leica Auto Stainer is the only way to go. It does all that you ask for and ours
is old and still a work horse.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Tuesday, October 19, 2010
Ours go on the VIP during the cleaning cycle. Then we dip them in a bucket of
alcohol containing mold release and spread them out to dry on a clean towel.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of We
Everyplace I have worked it was done by hematology. When hemo tried to dump
this job on us the pathologist stepped in to remind them that this is their
area of expertise. We do not expect hemo to do frozen sections and we do not do
bone marrows. After they have made their smears and sent the flo
Take some fresh tissue (we use umbilical cord) to micro and let them incubate
in for a few days in gram + and/or negative. Then fix it and you will have good
controls.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
We use 5 minutes on FG.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu
Sent: Thursday, April 08, 2010 6:42 PM
To: HistoNet
Subject: [Histonet] Masson Trichome Staining: Can I use Fast Gree
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