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Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
I would not use liquid nitrogen. The safest way to do this is to put the
blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove
caps, wrap in paper towels and hit with a hammer. Be sure to wear protective
clothing, eye covers and gloves. When the glass has broken
I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia
Water, wash for 5 minutes, dehydrate clear and mount if it is DAB. If it is
AEC I do the same except I rinse in distilled water before mounting with
aqueous mount.
Frances L. Swain HT(ASCP) A. A. S.
Special
I use Trypsin only as a last resort. I use Pepsin because it is more gentle
(does not chew up the sections). I do not use Proteinase K unless I absolutely
have to and for just a few minutes as it will eat the sections off of the
slides. If I use Citrate Buffer I heat it up in the microwave
What I do Kristen if I use it infrequently is I get a large brown bottle, label
it with working hematoxylin. After I use the hematoxylin for whatever I am
using it for I pour it in the brown bottle, cap it tightly and store it on the
shelf or in a cabinet. Whenever I need it again I get it
Hi Jenee: You need to check the histonet archieves. For the last two weeks
there has been an on going conversation in regard to disposal of microscopic
slides. Since I am in a COR lab most of my slides are sent to the PI's that
order the slides therefore I do not have to deal with this as of
Usually the cryoprotection is carried out after the specimens are fixed and
before they are frozen. If you have a sample you can spare you might try
making up some 20% Sucrose placing the frozen sample in it. putting it in the
refrigerator and letting it stay in the 20% sucrose until it drops
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfranc...@uams.edu email
-Original Message-
From: anh2...@med.cornell.edu [mailto:anh2...@med.cornell.edu]
Sent: Wednesday, March 18, 2009 8:41 AM
To: Swain, Frances L; Dr
There was a discussion about the Stable DAB on the histonet a few weeks ago. I
would suggest you search the archieves of the histonet. The company that has
this product was listed at that time.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic
If you want to remove the MMA you can also use 2-Methoxyethyl Acetate or
Acetone. If you want to decolorize like a hematoxylin, yes you can do that
also.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton
We wrap the bone in saline soaked gauze and freeze at -20 degrees C for bone
strength testing. If we receive bone for histomorphometric analysis we store
our bones in 100% Alcohol. If the Bones are for paraffin sectioning we fix for
at least 3 days wash in running tap water and store in 70%
There has been reference to someone else taking the practical exam instead of
the applicant. Since the written exam has become a computer generated program
what is to stop someone from taking the exam for an applicant. The reasons
given for dropping the exam are very shallow. The idea that
Dr. Chris Van der Loos has a wonderful textbook out that addresses these
issues. You can either buy the book or contact Dr. Van der Loos via the
histonet. He usually monitors this website. He has some excellent protocols
which work really nicely.
Frances L. Swain HT(ASCP) A. A. S.
Special
Hi Paul: If you will add charcoal to access to your Schiff's Solution swirl it
around and let it sit for about 15-20 minutes. Then filter it through a funnel
to which you have filterpaper with charcoal placed in the bottom it should come
out pretty clear. When we made our own we then placed
Sorry Paul I replied to Renee in stead of you. I purchase my Schiff's from
PolyScientific R D. When I made my Schiff's Used essentially the same recipe
that you do. I made mine in a flask stored it in the dark for 24 hours. I
then added activated charcoal to excess and let it sit in the
Joe and Pam have excellent points. We all need to advertise what we do. Here
in Arkansas when you tell the regular folks (non-medical) that you are a
Histology Technician. They ask do you do History research? I know that sounds
ignorant but the public is not very well informed as to the
Our reagent labeling policy is this. When you prepare a reagent for use such
as a buffer. The type of buffer is the first line, with the pH. The second
line tells what date it was prepared, if there was an expiration date and the
third line is the person who prepared it. When we receive new
We have purchased a flammable or explosion proof refrigerator from Fisher
Scientific. The thermostat is on the outside of the cabinent not inside the
cabinet. This is very important for two reasons: 1) if you have flammable or
ignitable reagents and you store then in a frost free refrigerator
Hi Cindy: We use 5% Formic Acid with a chemical end point test to determine
when decalcification has been completed. We make our own 5% Formic Acid but
Newcomer's Supply has some that I have used which worked really well. If you
have any questions please feel free to contact me.
Frances L.
We use the hematoxylin's from PolyScientific R and D from BayTown New York.
They are excellent. We use Harris', Weigert's, Mayer's and Gill's. They are
reasonable in price and the quality is outstanding.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic
You can possibly use it especially if it has been used for IF. What you need
to do is to get a slide that has a known positive for the antibody, try the
dilution for WB with your standard protocol and see if it works. If is too
light or too dark you will have to titer it (which should be done in
I cut MMA sections which are also hard to mount and have them stay on the
slides. I have found that Biocare, Surgipath, Newcomer's Supply and
Scientific Device Laboratory have what they call HIER slides or superplus
slides. All of them are great, the prices are a little steep.
Frances L.
Hi Karen: I have both microtomes. I use them to cut undecalcified MMA
sections. I like the Leica 2255 the best. I have a friend in our business
that uses to cut paraffin sections and thinks it is the best she has ever
used. I am almost in agreement with her although the Microm is also a
If you have a staining problem from the spill a weak solution of bleach
usually clears up any Schiff's that is dropped on the bench, floor, hands,
etc.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton
I work with non-decalcified and decalcified bone. After I have tried to
pressure cook it, steam it, microwave it, etc my sections look pretty
bad(coming off, etc.) I have found that either using Pepsin (Zymed) for 20
minutes at 37 degrees C. in a humidifying chamber or Pepsin for 20 minutes at
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