Dear Histonetters,
i've got few problem regarding the slide
preparation for laser microdissection. for your information, i'm using a
PEN-membrane from PALM to be used on laser microdissection pressure catapult
(LMPC), Ziess. According to the protocol i need to UV the slide prior
staining about 3
ored the
slide in slide box at RT (about 27degrees).
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1...@yahoo.com
From: "anh2...@med.cornell.edu"
To: shazana hilda ; histone
Dear Loralee,
Unfortunately I'm using DAB and yet still faded. This is the first time I'm
facing this problem.
Thank You.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1...@yahoo.com
___
I'm using DAB chromogen from LabVision and counterstained with Harris
Hematoxylin from Sigma .Then mounted using Cytoseal XYL from Richard-Allan
Scientific.
Thank You.
Shazana Hilda Shamsuddin
MSc.of Molecular Pathology,
Dept. of Pathology,
School of Medical Sciences,
Universiti
eatly appreciated.
Regards.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1...@yahoo.com
___
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Thank so much for all response. It's helps a lot.
Shazana Hilda Shamsuddin
MSc.of Molecular Pathology,
Dept. of Pathology,
School of Medical Sciences,
Universiti Sains Malaysia,
16150 Kubang Kerian,
Kelantan, Malaysia.
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: [EMAIL PROT
Dear all,
I've done the IHC staining step unfortunately encounter problem for a good
scoring system (i.e scoring that balance between cell distribution and staining
intensity).
Hope anyone who have experience in this matter can share they're opinion.
Thank you in advance.
Cheers
