Hello histonet!
Long time, first time.
My lab recently transitioned from hand staining for our routine H+Es to
using the Leica autostainer XL. The machine seems to work fine and the
protocol is nearly identical to our hand staining one. The problem we've
run into is that there is a streak of resi
The alum hematoxylins I've worked with required daily filtration. An
iridescent scum on top of the hematoxylin means you're overdue to filter.
When you brew a fresh batch of alum hematoxylin, it's a light yellow color.
As it oxidizes (slowly if you depend on atmospheric oxygen, instantly if
you ad
22, 2015 5:04 PM
To: gayle.cal...@bresnan.net; Histonet
Subject: Re: [Histonet] Hematoxylin Precipitate
Liz, Phillip and all that are
interested,
I take it you have guys never looked at or had someone else
examine what is at the bottom of the Hematoxylin filter after
you put through a day's wo
@lists.utsouthwestern.edu]
Sent: Tuesday, September 22, 2015 12:26 PM
To: Elizabeth Chlipala ; Tim H
Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Hematoxylin Precipitate
Wow, I agree with Liz. There should not routinely be "so much tis
, Unit E
Longmont, CO 80504
-Original Message-
From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 22, 2015 3:04 PM
To: gayle.cal...@bresnan.net; Histonet
Subject: Re: [Histonet] Hematoxylin Precipitate
Liz, Phillip and all that are
interested,
I
by no means is meant for any weak stomach
individuals or those preparing for the zombie apocalypse.
> From: gayle.cal...@bresnan.net
> To: thiggin...@msn.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Hematoxylin Precipitate
> Date: Tue, 22 Sep 2015 12:27:21 -0
2, 2015 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Precipitate
You should be filtering your Hematoxylin on a daily basis regardless of what
the manufactures says. We use to filter twice a day since we did a
traditional overnight run and then again in the afternoon for spec
tern.edu]
Sent: Tuesday, September 22, 2015 1:55 PM
To: Tim H; 'histonet@lists.utsouthwestern.edu'
(histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Hematoxylin Precipitate
I think you may a have a serious problem if "so much tissue washes off" if
that is happening you
1:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Precipitate
You should be filtering your Hematoxylin on a daily basis regardless of what
the manufactures says. We use to filter twice a day since we did a traditional
overnight run and then again in the aft
;Sandra Cheasty"
> To: "Histonet (histonet@lists.utsouthwestern.edu)"
>
> Subject: [Histonet] Hematoxylin Precipitate
> Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> He
n.edu]
Sent: Tuesday, September 22, 2015 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Precipitate
Dear Histonetters,
There is some *Denatured Ethanol* bottles in the lab I just started working
in (CDA, Fisher brand). Can this denatured ethanol be use
Dear Histonetters,
There is some *Denatured Ethanol* bottles in the lab I just started working
in (CDA, Fisher brand). Can this denatured ethanol be used for histological
processing? I have never used this product, only absolute pure ethanol.
Please advise.
Thank you very much
All the best
Alid
Yes, me too.
Turquois blue bacteria looking guys.
Trying to filter and use within a couple of days.
Has anyone mentioned this to their vendor?
Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On
Yes, I have had the same experience.Filtering it seems to help.
On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet
wrote:
Hello all,
Has anyone using Richard Allen Hematoxylin-2 noticed an odd
artifact on the slides after using the Hematoxylin for
Hello all,
Has anyone using Richard Allen Hematoxylin-2 noticed an odd
artifact on the slides after using the Hematoxylin for more than a few days on
their stainer? We are seeing small spore or pollen-like blue dots here and
there on the slides. It is not coming from the water b
Our new lot of Hematoxylin is too dark and especially overstains the mucins in
colon bxs and even membranes/umbilical cord.
Reducing the time from 3 minutes to 1 min helps some, but it the inappropriate
dark staining of the mucins is a real problem. We are also getting a film of
hematoxylin
We had a slight mistake with the deparaffin slides. A timer was not set,
long story short. The deparaffinized slides went too far and proceeded to
Hematoxylin. We have a linear stainer, so we took it back out from
hematoxylin when we noticed the error and placed it into water to rinse.
Then back to
Hi all you histonetters!
I had stained a couple of slides yesterday using H&E, but the staining came
out a little too light for my liking. Any set protocols for stripping the
stain and redoing it? (I use 7100 plastic resin). I've put the slides in
xylene to remove the coverslip/mounting medium, an
; From: janine.simmsco...@jmmc.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Hematoxylin issue
>
> Good afternoon all,
>
>
>
> I have been addressed with an issue which I would like some assistance
> with. My pathologist recently reviewed some skin s
Good afternoon all,
I have been addressed with an issue which I would like some assistance
with. My pathologist recently reviewed some skin slides from 2007 and
noticed the hematoxylin was completely gone and only the eosin remained.
We use Gill's II hematoxylin, and glass cover slips with xyl
Dear Histonet Members,
I am currently staining 20 um cryostat sections of brain tissue and visualizing
with DAB along with hematoxylin counterstain. For sections stained with the
same concentration of primary antibody, I seem to get lighter DAB staining for
slides with hematoxylin than ones wi
We had a similar thing happen last week, except in our case it was the eosin
was very pale & the hematoxylin was more blue than purple. Through our
troubleshooting steps, we determined it could have only been the processor
reagents. We fully changed it and the problem went away. While I am del
Is it only the HTX which is pale? Did you change anything else? Like
dewaxing?
anuradha shrivastava:
> Hello Every body,
> We changed the solutions testerday in the processor, and today Dr. complained
> about pale hematoxylin. Can u suggest what is wrong.
> thanks.
> anu.
> __
Hello Every body,
We changed the solutions testerday in the processor, and today Dr. complained
about pale hematoxylin. Can u suggest what is wrong.
thanks.
anu.
___
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http://lists.utsouthwestern.edu/
Can anyone suggest a good hematoxylin counterstain for central nervous
system tissue embedded in polyester wax? And provide a recipe?
Thank you
Luisa
Luisa Onstead-Cardinale, M.S.
Research Associate
UF Biomedical Research Laboratory, Room 121
655 West 11th Street
Jacksonville, Fl 32206
du
Subject: RE: [Histonet] hematoxylin-eosin saffron
In a good H&E, the eosin/phloxine is supposed to be 3 shades:
- RBC's/eosinophils/Paneth cells the deepest shade of pink
- muscle medium shade of pink
- collagen/connective tissue lightest shade of pink
Unfortunately, if the eosin and/o
n.edu] On Behalf Of Kalleberg,
Kristopher
Sent: Friday, April 16, 2010 1:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] hematoxylin-eosin saffron
Can someone please explain to me the purpose of a hematoxylin-eosin saffron
stain. Why would this be used over the normal hematoxylin and
--
From: "Kalleberg, Kristopher"
To:
Sent: Friday, April 16, 2010 1:44 PM
Subject: [Histonet] hematoxylin-eosin saffron
Can someone please explain to me the purpose of a hematoxylin-eosin
saffron stain. Why would this be used over the normal hematoxylin and
eaosin stain. Thank you
Can someone please explain to me the purpose of a hematoxylin-eosin
saffron stain. Why would this be used over the normal hematoxylin and
eaosin stain. Thank you in advance.
Kris
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists
Gill II (ThermoFisher) 5 min, 3x1 min A. Dest.
That's it :)
> I need some pointers on how to intensify the hematoxylin on the H and E
> stain. Is there a way to do that? I currently use Richard Allan hematoxylin
> 7211. The H is weak. All reagents were changed out last night. Any of your
> exp
From: DELIA GARCIA
To: histonet@lists.utsouthwestern.edu
Sent: Wed, November 11, 2009 10:12:19 AM
Subject: [Histonet] Hematoxylin
I need some pointers on how to intensify the hematoxylin on the H and E stain.
Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The
H is
I need some pointers on how to intensify the hematoxylin on the H and E stain.
Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The
H is weak. All reagents were changed out last night. Any of your expertise
would be of great help. Thank you guys in advance.
Delia
If hematoxylin is place on a slide before the nova red is it too late to add
the nova red after the hematoxylin?
Thanks
___
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Our lab is currently considering a swich from Richard-Allan Hematoxylin 1 (at
$154 per liter) to PremiumLine Hematoxylin (at $30.00 per liter). The
pathologists actually prefer the staining results from the cheaper source, but
are concerned about longevity - we are required to store the slides
We use ours for a few weeks, but we do filter it before use.
kristen arvidson
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/24/2009 07:48 AM
Please respond to
arvidsonkris...@yahoo.com
To
histonet
cc
Subject
[Histonet] hematoxylin Question
Hello All,
How long is hematoxylin
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Tuesday, March 24, 2009 9:44 AM
To: histonet
Subject: [Histonet] hematoxylin Question
Hello All,
How long is hematoxylin (we use Richard
Hello All,
How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is
poured into a staining container? The stain gets used so infrequently I would
like to keep it as long as I can. We currently dump it weekly regardless of
use. Thank you.
-Kristen
___
bottles and fill them again sharing the solution
equally between both.
René J.
--- On Tue, 2/17/09, Hana Peter wrote:
From: Hana Peter
Subject: [Histonet] hematoxylin
To: Histonet@lists.utsouthwestern.edu
Date: Tuesday, February 17, 2009, 1:41 PM
Hello!
I have two liters of hematoxylin Lillie
Hello!
I have two liters of hematoxylin Lillie Mayer modification, "home made" a
month ago. One bottle (1L) seems perfectly OK, but the other have some
precipitate (actually two or three very large crystals). They were stored in
the same cupboard at room temperature. Two weeks ago we were a few day
Tanya i've used several type of Hematoxylin in my entire life working in
histology.Different pathologist like different outcome of
hematoxylin.its hard to pleased everybody...but i always come back w/
one commercial Harris Hematoxylin made by Fischer..its w/ acetic acid and
m
you know that TBS works for bluing any hematoxylin.
Gene
-Original Message-
From: Kelly Boyd
To: histonet
Sent: Thu, 8 Jan 2009 2:46 pm
Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides
Biocare's Cat Hematoxylin is really good. One real nice feature is that you
)-830-0032
--- On Thu, 1/8/09, anh2...@med.cornell.edu wrote:
From: anh2...@med.cornell.edu
Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides
To: "Ring, Mary L" ,
histonet@lists.utsouthwestern.edu
Date: Thursday, January 8, 2009, 1:05 PM
I use Dako Mayer's fo
I use Dako Mayer's for 5 seconds followed by roughly a minute in bueing
solution. Gorgeous blue.
-Original Message-
From: "Ring, Mary L"
Date: Thu, 08 Jan 2009 09:50:00
To:
Subject: [Histonet] Hematoxylin Counterstain for IHC slides
Hello!
I am looking for a g
Hello!
I am looking for a good hematoxylin counterstain for IHC slides. My
pathologists are familiar with the bright blue color of Ventana's hematoxylin
counterstain, so I am looking for something similar.
Thanks for your help!
Mary
This e-mail and an
Yes. Lillie RD & Fullmer HM 1976. Histopathologic Technic
an= d Practical Histochemistry, 4th ed. New
York: McG= raw-Hill. Page 540, about one
quarter of the way do= wn the
page. John Kiernan Anatomy,
UWO London,
Canada= = = => -
Orig
Hello all,
Is there anything published on using H&E staining to presumptively indicate
calcification (primarily the hematoxylin)?
Thanks,
Albert
Albert C. Grobe, PhD
Tissue Engineering Lab
International Heart Institute of Montana Foundation
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