Hello,
Has anyone tried the Qproteome FFPE Tissue Kit kit from Qiagen:
Qproteome FFPE Tissue Kit
For isolation of full-length proteins from formalin-fixed tissues
* Standardized, quantitative extraction from FFPE tissue
* Full-length proteins
* Low sample requirements
The
Thanks so much, Theresa. I'll give you a call today. I appreciate your
help.
Carla
Carla Thompson
Senior Specimen Buyer
Microscope Slides Lab
a division of VWR International, LLC.
5100 West Henrietta Road
Post Office Box 92912
Rochester,
Hi all,
I just performed TRAP staining on ffpe edta delcaled bone sections using
the Sigma kit (#387A), and was wondering if anyone had experience with
reducing the yellow staining over the entire tissue due to the Fast Garnet
GBC. I had positive TRAP staining that is red, but there is a lack of
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
koelli...@comcast.net
Sent: Wednesday, April 16, 2014 9:53 AM
To: M.O.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block?
Hi Merissa,
don't know
Dear Histonetters,
I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung
sections?
I would appreciate any help.
Regards,
Mesru
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mesru T
Sent: Tuesday, 14 January 2014 5:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] myeloperoxidase on FFPE or frozen sections
Dear Histonetters,
I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung
To me, the critical data is: which Abs do you want to use on FFPWS of skin,
please?
If you do chromogen IHC on your skins already, with them Abs, you can do IF.
Sure, autofluorescence can be a problem but..not that big a problem.
Interestedly,
Carl
NB: that article is..strange.
Carl
.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Martin,
Erin
Gesendet: Montag, 18. März 2013 19:28
An: histonet
Betreff: [Histonet] Immunofluorescence on FFPE skin
Hello all,
My
Hi everyone, Several people have contacted me regarding the article I mentioned
in my post yesterday. Here are the details:
Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections
in the Assessment of Human Renal Biopsy Specimens
AJCP 2013 139:71-78;
Hello all,
My pathologist gave me a copy of Immunofluorescence with Dual Microwave
Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy
Specimens from the American Journal of Clinical Pathology. He said that the
same principle should work on skin and he would like
Hi all,
What's your favorite anti-CD45 for mouse... Serotec rat monoclonal or something
else?
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
Notice:
All,
I need a reliable fluorescent method for FFPE tissue using mouse monoclonal
antibodies. The secondary will need an emission of 488 and 594. I currently
use an HRP method with DAB and would like to visualize with fluorescent
method. Will only need single labeling technique. The following
@lists.utsouthwestern.edu
Sent: Sunday, October 21, 2012 11:20 AM
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax
, 2012 11:20 AM
To: 'Mark Elliott'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Friday, October 19, 2012 3:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 for FFPE IHC on Mouse
Amy
Can you please share the responses you got with the rest of us
Thanks
Mark
Message
From: Amy Porter port...@msu.edu
To: 'Histonet' histonet@lists.utsouthwestern.edu
Sent: Thursday, October 18, 2012 12:35 PM
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might
Amy
Can you please share the responses you got with the rest of us
Thanks
Mark
Message: 8
Date: Thu, 18 Oct 2012 16:21:55 -0400
From: Amy Porter port...@msu.edu
Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse
Model
To: 'Amy Porter' port...@msu.edu,'Histonet'
histonet
18, 2012 3:36 PM
To: 'Histonet'
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.
Amy S. Porter, HT
high-avidity/affinity
antibodies to the target antigen.
Good Luck,
Joe Myers, M.S., CT(ASCP)QIHC
--
Message: 3
Date: Thu, 29 Sep 2011 13:10:58 -0400
From: mesruh turkekul turke...@gmail.com
Subject: [Histonet] Background in FFPE glandular tissue sections
during
Dear Histonetters,
When I do IHC on FFPE tissue sections. Most of time there is background due
to trapping of IgG/detection reagents probably in the connective tissue
fibers, mucins or other secretions of the glandular tissue. Especially
prostate and mammary gland. Any tips to get rid of that
Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
René J.
--- On Fri, 5/14/10, Morken, Tim timothy.mor...@ucsfmedctr.org wrote:
From: Morken, Tim timothy.mor...@ucsfmedctr.org
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: Perry, Samuel samuel_pe
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, May 15, 2010 7:36 AM
To: SamuelPerry; histonet@lists.utsouthwestern.edu; TimMorken
Subject: SPAM-LOW: Re: [Histonet] RE: Prolonged FFPE slide and block storage
Inexpensive long term
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, May 15, 2010 9:36 AM
To: SamuelPerry; histonet@lists.utsouthwestern.edu; TimMorken
Subject: Re: [Histonet] RE: Prolonged FFPE slide and block storage
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage
Hi All,
We have a growing need
What antibodies are people using for this marker?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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PG-M1 from dako
2009/11/18 Kim Merriam kmerriam2...@yahoo.com
What antibodies are people using for this marker?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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.
19) Let sit in tap water for 5 minutes.
20) dehydrate and coverslip in accordance with SOP’s.
Best of luck,
Bob Schoonhoven
From: Patsy Ruegg pru...@ihctech.net
To: histonet@lists.utsouthwestern.edu
Sent: Friday, April 17, 2009 1:26:36 PM
Subject: [Histonet
histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] CD31 on FFPE mouse tissue
What is everyone doing for CD31 on FFPE mouse tissue? We are looking to
do IF, but a DAB protocol would be just as good.
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
would contact Biocare.
Kim Merriam kmerriam2...@yahoo.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/13/2009 12:27 PM
Please respond to
kmerriam2...@yahoo.com
To
Histonet histonet@lists.utsouthwestern.edu
cc
Subject
[Histonet] CD31 on FFPE mouse tissue
What is everyone
I have used the antibody and kit from Biocare for CD31 and got good results.
Simple to use.
nancy.lo...@med.va.gov
--- On Fri, 2/13/09, Jackie M O'Connor Jackie.O'con...@abbott.com wrote:
From: Jackie M O'Connor Jackie.O'con...@abbott.com
Subject: Re: [Histonet] CD31 on FFPE mouse tissue
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