Hello all,
Yes, the Leica Bond and Bond Max has an Alk Phos detection kit available.
Though the red can bleed if left in alcohol, it is more stable than other
branded red detection kits. The Red kit is also very stable for long term
storage and exceedingly better than the competitor brands that
Hello,
I am searching for veterinary labs who are using the Leica Bond to perform TSE
testing following the NVSL protocol. Specifically did you or are you still
having issues passing the check test?
Thanks
Roger
Roger Charles| Microbiologist II
Pennsylvania Veterinary Laboratory
2305 North
Hi Paula,
Are you using the Leica Bond for Clinical diagnostic testing? If yes, use of
any biomed company to PM or use non-Original equipment manufacturing (OEM)
parts on the instrument would void the IVD status of your instrument and any
IVD reagents you are automating on it.
Sent from
Hi all,
Does anyone know of a Biomed company that makes and/or sells parts for the
Bond III and the ASP6025 processor?
It's amazing how much Leica wants for their parts. For example, the waste
bottle used to collect the buffer waste is $674 and the seal for the
retort's lid is $597.
Hello Histonetters,
I wanted to find out how you are cleaning your covertiles that are used for
chromogenic & Immunofluourescent IHC as well as RNAscope?
Thank you!
Kate
This message is intended solely for the designated recipient(s). It may contain
confidential or proprietary information
Hello,
I was referred to join this listserv due to a question we have about our
Leica Bond Max. I work in a medical diagnostic laboratory. Does anyone know
where we can get good quality but cheaper reagents to use on the Leica Bond
Max, perhaps from a third party? The prices from Leica
Hi,
Is anyone experiencing problems with the Bond 3 immunostainer software
version 6.0.0.365 and the new Leica labels for the BOND. We have 3 BONDS in
our department and have used the BOND platform for over 10 years.
Previous software versions have been much more user friendly.
Regards
Hi all,
Is there anyone interesting to the Leica bond max IHC stainer?
Thanks,
Kai Hong
Research histotechnologist
kh...@waxitinc.com
202 - 2386 East Mall
Vancouver, B.C.
V6T 1Z3 Canada
Office: 604-822-1595
Lab: 604-822-2589
Fax: 604-822-0660
www.waxitinc.com
-Original Message-
-
From: Greg Dobbin
Sent: Thursday, October 11, 2018 11:04 AM
To: linda.marg...@cookchildrens.org
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica Bond Max artifact
Hi Linda,
Is this a problem that has just started or has the red kit always had this
artefact?
- If it just
Hi Linda,
Is this a problem that has just started or has the red kit always had this
artefact?
- If it just started, perhaps it is a problem with a particular kit lot
number (or numbers). If so, get Leica Tech service involved to see if
anyone else has reported a problem.
- If it
Hello Dr. Rosen and Histonetters,
We have used a Leica Bond RX for over 2 years now, cleaning and caring for it
just as you do. My work is mostly animal which is why we mainly choose the red
chromogen alkaline phosphatase detection kit. We see the same artifact
sporadically. Sometimes we
Hi Histonetters,
Leica is recommending not to use clipped corner slides on their Bond Rx
instrument. I know there are labs out there who use this instrument and may
also use clipped corner slides in their slide printer. For those labs, do you
have any problem with covertiles not working
We've been adapting the Leica Bond to do immunofluorescence staining and
finding it a hard road. Leica does not support IF staining within the standard
clinical software so we need to buy a FISH kit (just one vial of formamide) in
order to get a "kit" barcode to run the system. We dump the
our issue.
>
> From: Charles Riley via Histonet [histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, December 15, 2015 6:21 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Leica Bond machines
>
> Hello all,
> Does anyone use the Lei
Check the pH of your water. If it's LT 5.5, that could be your issue.
From: Charles Riley via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, December 15, 2015 6:21 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond
Hello all,
Does anyone use the Leica Bond Max and or Bond III? I am having an issue
with the counterstain being really light. There has been no change in the
detection kits or the programs being run, however the counterstain has
become weak sometimes and other times its perfectly fine. Any ideas
Hello everyone, hope this Monday is treating you well. I am in dire need of
some assistance. We are a dermpath lab in Knoxville, TN using the Leica Bond
for our IHC. We have been unable to acquire our Refine Red Detection Kits due
to some technical difficulty. I am looking for anyone who
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, and reply to the sender
regarding the error in a separate email.
-Original Message-
From: Miller, Wendy [mailto:miller.we...@mhsil.com]
Sent: Wednesday, July 08, 2015 9:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica bond
Hi! Does anyone out there use the Leica IHC Bond
@lists.utsouthwestern.edu
Subject: [Histonet] Leica bond
Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into
purchasing one and I would like to input. Do you like it? Pros and cons?
Thanks
This message (including any attachments) contains
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica bond
Wendy, the Leica Bond is a great machine. We've had it for several years now
and it provides excellent and consistent results.
Paula
Bio-Path Medical Group
Fountain Valley, CA
-Original Message-
From: Miller, Wendy [mailto:miller.we...@mhsil.com
AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica bond
Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking
into purchasing one and I would like to input. Do you like it? Pros and
cons?
Thanks
This message (including any
Hi All,
I am not a Bond user, but have an antibody were a user had success using
standard protocol F
Can some please enlighten me with the specifics of that protocol?
Many thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck Co., Inc.
PO Box 4, WP-44K
West Point,
Good Morning, I have a question about the Leica Bond Max reagents. Has anyone
ever heard of purchasing the reagents from a 3rd party rather than from Leica ?
If so, what is the company's contact info.
Thanks for your help.
Marilynn Weber H.T.( ASCP ) QIHC
Coastal Pathology Consulting
Forwarding to Histonet:
Hi Roger,
The Bond IHC unit is used in two of the laboratories where I work. My hands-on
experience is limited since I'm the new kid on the block but here's what I've
learned, from my mistakes. The unit does not know if the covertiles are in
place or not and the
Love the bond it does not break down very often for me and service is great i
can run all the antibodies i want for me even research animal because i have a
more open research unit cleaning the covertiles is a little tedious but we make
it work and replace them with new ones often beats
The bond is great. A lot less waste to deal with and the maintenance is s
easy. Less than 10 minutes. No tubing to unclog and disinfect. And the
operating cost is much cheaper.
And to top it off, it won't even give you the option to start a run until
everything is right. No going back and
Could I get the opinions of any labs using the Leica Bond III for IHC staining?
I'm especially
interested to hear from those that switched from Ventana to Leica, but any
feedback is appreciated.
Few questions that come to mind:
Are you happy with the quality of the stains you're performing on
Good Day everyone,
Is anyone using the new Leica Bond RX research IHC/ISH autostainer? If you
are I would love to hear any feed back on it you may have.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone:
Hello all,
Our laboratory is considering a purchase, and Leica will be giving a
presentation on the unit in September. I was hoping to get some responses of
pros/cons/quirks from users to help in our assessment. We are currently using
Ventana XT to look at PD biomarkers in cell lines,
The Bond III and Bond max are great. The Bond III has a shorter run time. Not
to mention the cost savings on both machines. And yes, maintenance is a piece
of cake. Good luck!
Sent from the iPhone of Kim Tournear
On Aug 22, 2012, at 8:13 AM, Crowell, Thomas thomas.crow...@mpi.com wrote:
Use it everyday. Are you looking for opinions?
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: Wellen Terrence D. :LPH Lab twel...@lhs.org
Date: Thu, 5 Apr 2012 00:06:04
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond IHC Platform
Does anyone have
Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra.
judith_par...@memorial.org
Judith Pardue
This message and accompanying documents are covered by
the Electronic Communications Privacy Act 18
U.S.C. Sections 2510-2521, and contain information
intended for the
@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond verses the Ventana Ultra
Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra.
judith_par...@memorial.org
Judith Pardue
This message and accompanying documents are covered by
the Electronic Communications
We love our Bond too.
Muhammad Tahseen
Histology supervisor
SKMCH RC
Lahore Pakistan
Need to know histotechs opinion of the Leica Bond verses the Ventana
Ultra.
judith_par...@memorial.org
Judith Pardue
This message and accompanying documents are covered by
the Electronic
I want to poll Leica Bond III users to see if they are able to successfully run
EBER Insitu on their unit?
We continue to get inconsistent staining and inconsistent background staining.
I have been told it's a pH problem, it's a tissue processing problem but we are
able to do the stain with
Hello netters:
Looking for opinions on the Leica Bond immuno stainer please.
Thanks.
Sheila
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We are very happy with our BOND and technical support has been great for any
and all questions/issues.
Nancy Schmitt HT,MLT(ASCP)
Dubuque, IA
--
Message: 16
Date: Sat, 25 Jun 2011 12:00:44 -0400
From: Sheila Adey sa...@hotmail.ca
Subject: [Histonet] Leica Bond
Subject: [Histonet] Leica Bond for IHCs
Hello netters:
Looking for opinions on the Leica Bond immuno stainer please.
Thanks.
Sheila
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Hi Histonetters
I am testing the Leica Bond III immunostainer. How is everyone handling
processing the Hercept test from Dako on the Bond III? I cannot use either
the approved pretreatment or visualization system, not to mention the
approved buffer. I currently run my immunos on the Dako so it
We have a Bond III and we love it. I don't usually use the word love for an
instrument, but, in this case, it applies. We do multiple runs on it every
day. We were a pilot site for Leica-Microsystems and at the end of the test
period I told them that I didn't want this instrument to leave.
...@lists.utsouthwestern.edu]on Behalf Of Richard
Cartun
Sent: Wednesday, January 27, 2010 3:59 PM
To: Histonet; Victor Tobias
Subject: Re: [Histonet] Leica Bond 3
We have a Bond III and we love it. I don't usually use the word love for an
instrument, but, in this case, it applies. We do multiple runs on it every
day
Hey everyone
I have a question regarding the Leica Bond IHC stainer. We are
currently evaluating it. Is it true that you can not use a protein
block on the instrument and if you do you have to rinse afterwards. I
know that a lot of labs do not use protein blocks anymore but we
routinely
Fax# 858-678-8290
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Tuesday, December 15, 2009 3:21 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Leica Bond
Hey everyone
I have
Hello,
We are considering the Leica Bond and their reagent rental or acquisition
option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents (novocastra),
are there any problems with back orders on antibodies, detection system and
-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 05, 2009 11:20 AM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question
: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back
: [Histonet] Leica Bond/Novocastra Reagents
Hello,
We are considering the Leica Bond and their reagent rental or
acquisition option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents
(novocastra), are there any problems with back orders
Almost always receive on time. I can think of only one instance in a
year and a half where anything was on back order (it was an antibody).
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle
MacVeigh-Aloni
Sent: Tuesday, March 31, 2009 8:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond Max - any opinions?
Hi all,
I
@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
RE: [Histonet] Leica Bond Max - any opinions?
I would really suggest you look closely at the Intellipath from BioCare.
They are a truly open system.
Linda
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Michelle,
We also only work with animal tissue. Have you considered researching
Biocare's Intellipath immunostainer? We also love their products and this
is an open system. I am not the one who made the purchase decision or
used both of the stainers, but, although the Leica immunostainer was
Hi all,
I am researching the Leica Bond Max immunostainer and I like its features as
advertised.
The advertisement however states that the system is completely open, but after
discussing it in length with the Leica rep I am finding that this is actually
not so...
We are a research core and
I have been evaluating the Leica Bond IHC stainer and their tech support
Pauline Wong is absolutely wonderful. Will also be evaluating the Intellipath
by Biocare in December. Any comments greatly appreciated!
Thank you,
Angela
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