Re: [Histonet] PCNA sc-7907?

2012-07-04 Thread Mehmet Fatih BOZKURT
dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... On Wed, Jul 4, 2012 at 10:59 PM, Mehmet Fatih BOZKURT wrote: > dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... > > On Wed, Jul 4, 2012 at 10:21 PM, E Wolfe wrote: > >> Hello Histonet - >> >> Has anyone had an

[Histonet] PCNA sc-7907?

2012-07-04 Thread E Wolfe
Hello Histonet - Has anyone had any success staining with PCNA sc-7907 on frozen, paraformaldehyde-fixed tissue? I am experiencing a lot of background, and so far diluting down to 1:5000 hasn't helped at all. Any advice would be very much appreciated! __

[Histonet] PCNA stain

2011-05-24 Thread Pam DeFazio
One of our pathologist has ask me to find somewhere to do this stain. I would like to send unstained slides for staining only, he will do the intreperation. Thanks for your help! Pam DeFazio, HT (ASCP) Athens Regional Medical Center Athens, GA ___ Hi

Re: Re: [Histonet] PCNA

2009-12-02 Thread TF
we use the cell signaling one. PCNA of mouse IgG 2009-12-02 TF 发件人： Richard J 发送时间： 2009-12-02 19:04:06 收件人： linresearch 抄送： histonet 主题： Re: [Histonet] PCNA Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish Protocols and images at http

Re: [Histonet] PCNA

2009-12-02 Thread Richard J
Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish Protocols and images at http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=PCNA 2009/12/2 > > > Hello, > Can anyone recommend PCNA antibody that works in FFPE rat tissue? > Woul

[Histonet] PCNA

2009-12-01 Thread linresearch
Hello, Can anyone recommend PCNA antibody that works in FFPE rat tissue? Would you be willing to share your protocol? thanks, Lin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] PCNA staining on paraffin

2008-12-11 Thread anh2006
Second this, the DAKO clone works great. -Original Message- From: Jan Shivers <[EMAIL PROTECTED]> Date: Thu, 11 Dec 2008 10:01:38 To: Merced Leiker<[EMAIL PROTECTED]> Cc: histonet Subject: Re: [Histonet] PCNA staining on paraffin I use Dako's PCNA (cat. # M0789),

Re: [Histonet] PCNA staining on paraffin

2008-12-11 Thread Jan Shivers
December 10, 2008 1:07 PM Subject: [Histonet] PCNA staining on paraffin Does anyone have any suggestions for staining PCNA on paraffin? We are using Santa Cruz's PCNA (FL261) SC-7907. We have already stained with it successfully on frozen sections, but it does not appear to be working on

RE: [Histonet] PCNA staining on paraffin

2008-12-11 Thread Robert Schoonhoven
L PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Merced Leiker Sent: Wednesday, December 10, 2008 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PCNA staining on paraffin Does anyone have any suggestions for staining PCNA on paraffin? We are using Santa Cruz's PCNA (FL261)

[Histonet] PCNA staining on paraffin

2008-12-10 Thread Merced Leiker
Does anyone have any suggestions for staining PCNA on paraffin? We are using Santa Cruz's PCNA (FL261) SC-7907. We have already stained with it successfully on frozen sections, but it does not appear to be working on paraffin. Thank you. Merced M Leiker Research Technician II 354 BRB (pkgs) /