Greetings , fellow Histonetters,
I'm reaching our for advice on education. I've worked in research histology
ages ago, set up a lab and did everything from organ harvesting and
grossing to processing, staining ( routine, special, IHC, and IF). Then I
went the management route and have been in lab
Katherine:Our lab did the same thing a few years ago and I received operating
instructions for this system from a sales rep with Biocare that I know. I'll
send this document to you offline. I can also put you in touch with the guy
from Biocare. As Colleen said, Biocare doesn't sell or
The Biocare certain was called the Nemesis. They are phasibg them out but
might be willing to come assist you.
Colleen Forster
On Thu, Oct 24, 2019, 2:43 PM Eileen Akemi Allison via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Ask Biocare or Dako. Unbeknownst to most of the public,
Ask Biocare or Dako. Unbeknownst to most of the public, it is the same exact
unit they had years ago. Lab Vision made all 3 units. Their brand, Biocare and
Dako. Lab Vision just put different branding on the various units when they
were sold.
Akemi Allison-Tacha, BS, HT/HTL (ASCP)
Former
Hello Histonetters,
My company has acquired a refurbished ThermoFisher Lab Vision autostainer 3602D
and I have questions on how to operate. It is no longer supported by
ThermoFisher so I am unable to get tech support through them. Is there anyone
who is familiar and available/willing to
t;false negatives".
Gudrun
leading histotechnologist,
Kepler Universitätsklinikum Linz
Austria
-Ursprüngliche Nachricht-
Von: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Freitag, 6. Januar 2017 18:26
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet
Is there anyone in Histonet land who is using both the Optiview and Ultraview
on the XT and ULTRA at the same time? I'd like to ask a few questions, please
email me.
We have a cytology cellblock case that came out stunningly positive for Her2
and one of our pathologist thought it would
Happy New Year Everyone!! :)
Questions on CAP Anatomic Path Checklist. Are the microwave questions referring
to microwave processors/processing or the microwave oven that we use for
special stains? Questions are ANP. 27170, 28290, 28860, and 29430.
I'm thinking the microwave oven but I want
Hello Histo-netters!
I have a Bond RX and before I try to recreate the wheel, I thought I would ask
some experts. Has anyone used a goat primary on this instrument and if so how?
Also, has anyone had success with TUNEL on this instrument and if so, could
you share your knowledge?
Thank you
I have a couple of questions to ask where there is no right or wrong answer,
just curious as to the process that other labs use.
1. After processing, how do you determine the order in which to cut and stain
the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours
of
*
Message: 17
Date: Thu, 7 Aug 2014 11:54:00 -0500
From: Webb, Dorothy L
dorothy.l.w...@healthpartners.commailto:dorothy.l.w...@healthpartners.com
Subject: [Histonet] questions
To: 'histonet@lists.utsouthwestern.edu'
histonet
From: dorothy.l.w...@healthpartners.com
To: histonet@lists.utsouthwestern.edu
Date: Thu, 7 Aug 2014 11:54:00 -0500
Subject: [Histonet] questions
I have a couple of questions to ask where there is no right or wrong answer,
just curious as to the process that other labs use.
1
So here at TJUH, we are color coded and all blocks other than routine and
decals are cut in this manner. One tech is assigned rapids (green), another
tech is assigned bone marrow and cell blocks (yellow and blue) and one tech is
assiged out satalite hospital (pink cassettes) All are
Hi Everyone.
I am trying to troubleshoot my IHC on frozen sections.
My sections are human tonsil at 7 uM. On charged Superfrost slides.
They are stored at -80 after drying for 1 hour.
When I use them for IHC, I take them out of the -80 and let them air dry for 1
hour before placing them in
For those of you who use reagent alcohol, have you ever experienced any
problems in processing or staining, such as artifacts, crystals forming, etc??
How long are unstained slides usable? Do any of you pick up extra sections
from a ribbon if the tissue is minimal ? We do and have used them
Dorothy, saving cut slides forever is not a good idea in my opinion. They
attract dust, fungus,Bacteria, all kinds of artifacts on top of loosing antigen
specificity. Cut slides is not the answer to me. I had to deal with this a
couple if times hopefully your pathologist will understand it's
www.mps.com.au
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Thursday, 23 February 2012 5:18 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions
For those of you who
I originally asked my questions because I *knew* it was being done
incorrectly and no one @ my workplace believed me when I tried to show
them the way I was taught/trained-as stated in my original post.
Regardless, it never occurred to me that my questions were something
that would be met with oh
We have been having problems with underprocessed placental membrane, some of
which are cut fairly thick, but am seeing it on more than just the thich
samples. Does anyone out there in histoland have a special process for
placentas or any helpful hints? I do know that many times the placentas
: Tuesday, March 30, 2010 3:04 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions
We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick, but am seeing it on more than just
the thich samples. Does anyone out there in histoland have
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Wednesday, 31 March 2010 6:04 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions
We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick
I have pondered and edited the answers I've gotten from many of you and,
in response to some very pointed suggestions that EVERYTHING be shared
on Histonet to further communication and professional bonding, here
are the questions for the Hypothetical Interview of a Pathologist:
1. What is
Hello to the Histotects community worldwide.
Would like to know if someone out there has any suggestion or have worked
with nematode larva:
1- fixation and after (protocol)
2-permeabilization of the chitin outer membrane(protocol)
in order to:
3- adhere them to slides (maybe polylisine
Jacqui Detmar wrote:
Hi all. Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please. Here are the
questions:
1.When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?
Hi all. Having a bit of an internal debate here, so I would like to get
the opinions of some of you in Histoland, please. Here are the
questions:
1.When fixing with 10% NBF, for how long should you fix and what
volume ratio of fixative:tissue should you use?
2. At what
1. Generally 24 hrs for 2-3 mm thick piece. At least a 10:1 formalin:tissue
volume.
2. Room temp or 4oC - I've heard debates, and I've heard it doesn't matter,
but we do it at 4oC.
--On Tuesday, March 24, 2009 4:54 PM -0400 Jacqui Detmar
det...@lunenfeld.ca wrote:
Hi all. Having a bit
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