tonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. They want 10 - 10u sections in
a micro-centrifuge tube. The only way to get the sections in the tube is
for the sections to roll. How do you get sections to roll when yo
I have some researchers that want to do PCR. They want 10 - 10u sections in a
micro-centrifuge tube. The only way to get the sections in the tube is for the
sections to roll. How do you get sections to roll when you want them to roll?
I've tried room temperature, on ice, brand new sharp blad
At one time, when we were using knives (not disposable blades), and we were
using a roller plate, I found that the Shandon (now Thermo Scientific) M1
Embedding Matrix:
http://www.thermo.com/com/cda/product/detail/1,1055,21902,00.html appeared to
cause less rolling than OCT. You could try that.
If your cryostat is set up correctly (ie, the roll plate isn't causing the
rolling up), then I would try 100% EtOH instead of 70%, I have no idea why
it works but sometimes, it does.
Also, when our cryostat starts doing this, I just clean the blade and the
knife and walk away for a few minutes--but
stern.edu] On Behalf Of Robyn Vazquez
>Sent: Wednesday, April 29, 2009 9:04 AM
>To: Dearolf, Jennifer; histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] Rolling sections
>
>Jennifer,
>It sounds like the sections are a little thick. Have you tried just
>barely blow
,
Jennifer
Sent: Tuesday, April 28, 2009 3:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rolling sections
Greetings, Histonetters!
First, I wanted to thank all of you that responded to my e-mail a few years
back about freezing small pieces of muscle tissue. We have found a method
9 11:00 AM
Please respond to
ti...@foxmail.com
To
"Dearolf, Jennifer" ,
"histonet@lists.utsouthwestern.edu"
cc
Subject
Re: [Histonet] Rolling sections
I also have problems in mounting brain sections...10um-30um in Leica
Cryostathope anyone can share their experience
发送时间： 2009-04-29 06:56:50
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] Rolling sections
Greetings, Histonetters!
First, I wanted to thank all of you that responded to my e-mail a few years
back about freezing small pieces of muscle tissue. We have found a method that
works for
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rolling sections
Greetings, Histonetters!
First, I wanted to thank all of you that responded to my e-mail a few
years back about freezing small pieces of muscle tissue. We have found
a method that works for us, and if anyone is interested, I
Greetings, Histonetters!
First, I wanted to thank all of you that responded to my e-mail a few years
back about freezing small pieces of muscle tissue. We have found a method that
works for us, and if anyone is interested, I would be happy to share. It still
involves the wonderfully explosive
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