I've been doing this PSR stain for a few years now on various types of tissue
sections of pretty much routine thickness (4-5 microns). I was asked today if
the stain would penetrate a thicker section - like 40 or more microns. Even a
vibratome section.
The tissue is pig optic nerve and a small b
l.
> René J.
>
> --- On Tue, 3/3/09, Vanessa J. Phelan wrote:
>> From: Vanessa J. Phelan
>> Subject: [Histonet] Section thickness
>> To: histonet@lists.utsouthwestern.edu
>> Date: Tuesday, March 3, 2009, 9:14 AM
>>
>> Hi Guys,
>>
>> Just wo
> Just wondering what thickness you cut sections at?
3 microns for all paraffin...
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
n.edu] On Behalf Of Jan
Shivers
Sent: Tuesday, March 03, 2009 10:17 AM
To: Vanessa J. Phelan; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Section thickness
4 um here.
Jan Shivers
UMN VDL
- Original Message -
From: "Vanessa J. Phelan"
To:
Sent: Tuesday, March 0
4 um here.
Jan Shivers
UMN VDL
- Original Message -
From: "Vanessa J. Phelan"
To:
Sent: Tuesday, March 03, 2009 8:14 AM
Subject: [Histonet] Section thickness
Hi Guys,
Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in m
...I just want to add, I could see it being a problem only in the sense
that thicker sections tend to give more background in your
immunostaining...however, you could conversely get greater signal strength
as well...
--On Tuesday, March 03, 2009 9:14 AM -0500 "Vanessa J. Phelan"
wrote:
Hi
I've read that 4-5um is "average", and that's what I typically go by for
paraffins, but then it could depend on tissue type as Rene recommended. I
end up having to cut frozens at 8-10um because for some reason it's very
difficult to cut any thinner on the cyrostat I use (an old Vibratome
model
ll prevail.
René J.
--- On Tue, 3/3/09, Vanessa J. Phelan wrote:
From: Vanessa J. Phelan
Subject: [Histonet] Section thickness
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 3, 2009, 9:14 AM
Hi Guys,
Just wondering what thickness you cut sections at? I was always used to
cutting at
Hi Guys,
Just wondering what thickness you cut sections at? I was always used to
cutting at 2-3 microns in my last lab, however in my new place they are
cutting at 6 microns (for both H & Es and IHC), which seems to me as really
quite thick! What would be the average cutting thickness?
Thanks a m
Joyce - Our lab cuts tissues from a variety of sources. We cut most tissues
at 3 microns. Since we take a lot of pictures and cells were considered 4
microns thick, 3 microns shows only on thickness and good for pictures. The
exceptions are kidneys, cut a 2 microns and CNS tissues cut at 5-6
General routine = 5 µm
Brain = at least 8 µm
René J.
--- On Thu, 10/9/08, LINDA MARGRAF <[EMAIL PROTECTED]> wrote:
From: LINDA MARGRAF <[EMAIL PROTECTED]>
Subject: [Histonet] section thickness
To: histonet@lists.utsouthwestern.edu
Date: Thursday, October 9, 2008, 1:49 PM
Histonette
ssage-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of LINDA
MARGRAF
Sent: Thursday, October 09, 2008 1:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] section thickness
Histonetters:
Here's a query from Janet that she couldn't get to post to the list.
Histonetters:
Here's a query from Janet that she couldn't get to post to the list. Please
respond to the list so she'll see the response. Thanks.
I would like to poll the group regarding routine practices for thickness of
paraffin sections. I am particularly interested in brain, spinal cord, lym
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