[Histonet] Silver ISH

2018-10-17 Thread Kurth Virginia L. via Histonet
Hello Jacqueline I am going thru old histo net messages and was wondering if you had any new insight on the silver ISH for HPV testing on the Ventana ultras, my lab would like to validate it, but I am worried about the consistency of the silver staining. Thank you, Ginny Kurth, UWHC, vku...@uwhe

[Histonet] Silver disposal

2018-06-06 Thread Laurie Colbert via Histonet
I'm wondering how others dispose of their silver waste from a GMS stain?  We have always collected and disposed of it separately from our other chemical waste.  We have a 55 gallon drum where we dispose of formalin, alcohol and xylene, and I was told by several people that we could just add the

[Histonet] Silver waste

2018-03-26 Thread Histology via Histonet
Hi All. Happy Monday! Would anyone share how they collect, store, and dispose of silver waste from manual special stains such as GMS, Melanin Fontana-Masson, and Calcium Von Kossa? Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthw

Re: [Histonet] silver nitrate

2014-07-01 Thread Maxim Peshkov
Tasha, 1. Try 10% Potassium Iodide (KJ) for 5-10 minutes, spot will turn to yellow as lemon, then apply 10% Sodum Thiosulfate, wipe it and then talc powder and dry. 2. 15% sodium chloride with some drops hydrochloric acid, then apply 10% Sodum Thiosulfate, wash and dry. Maxim Peshkov, Russia, Tag

RE: [Histonet] silver nitrate

2014-07-01 Thread Campbell, Tasha M.
11:23 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] silver nitrate You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second

Re: [Histonet] silver nitrate

2014-07-01 Thread Sue
You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second one is a little more difficult to work with but does work ___

[Histonet] silver nitrate

2014-07-01 Thread Campbell, Tasha M.
So does anyone out there have a secret formula to get silver stain off counter tops? Thanks for you the help!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) __

RE: [Histonet] Silver in Nucleolus

2014-01-23 Thread Tony Henwood (SCHN)
nal Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jerry sedgewick Sent: Friday, 24 January 2014 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver in Nucleolus I'm working with a lab that st

[Histonet] Silver in Nucleolus

2014-01-23 Thread jerry sedgewick
I'm working with a lab that stained cat gonads with Warthin Starry. We're looking for Bartonella bacteria. In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images. In the second batch it appears that nucleolus filled with silver.

[Histonet] Silver Gold intensification

2011-08-18 Thread Candice Smoots
Hey All   Does anyone have a silver gold intensification procedure that you could send me. I am doing this for the first time and I am not sure of what the tissue is supposed to look like.   If at all possible, could u send the protocol as well as guidelines as to what this tissue is supposed t

RE: [Histonet] silver stain on slide-mounted FFPE brain tissues

2011-04-26 Thread Hugh Luk
It sounds interesting. Hope this helps, Hugh, HTL (ASCP) Pathology core manager Hawaii > From: robin_d...@compbio.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 25 Apr 2011 15:09:49 -0700 > Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues > &g

[Histonet] silver stain on slide-mounted FFPE brain tissues

2011-04-25 Thread Robin Dean
Hi Histonetters, We want to do a degeneration-selective silver stain on slide-mounted formalin-fixed paraffin-embedded monkey brain sections. It looks like most of the kits/protocols are for free floating tissue sections or chunks of tissue that are stained and then sectioned. Has anyone used

RE: [Histonet] Silver

2010-09-07 Thread Ingles Claire
bject: [Histonet] Silver I know... I should wear gloves when doing a GMS.I... know... that. (sorry, I thought this was Facebook for a second) Have a great week! - Sir Bill of the Blackened Thumb ___ Histonet mailing list His

[Histonet] Silver

2010-09-07 Thread O'Donnell, Bill
I know... I should wear gloves when doing a GMS.I... know... that. (sorry, I thought this was Facebook for a second) Have a great week! - Sir Bill of the Blackened Thumb ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.ut

Re: [Histonet] Silver Lips and Fingers

2010-03-25 Thread Andrea Grantham
Beauty mark - like Cindy Crawford has. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicin

[Histonet] Silver Lips and Fingers

2010-03-23 Thread Breeden, Sara
I'm not a chemist and may shoot myself in the foot here, but if gold chloride tones silver, would it work on skin? Then you could call it a beauty spot? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _

RE: [Histonet] Silver Nitrate instead of inking??

2009-12-17 Thread Nancy Klemme
, 2009 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey H

Re: [Histonet] Silver Nitrate instead of inking??

2009-12-17 Thread Rene J Buesa
into a black stain. but that will not readily "fly" in a lab setting. René J. From: sheila adey To: histonet@lists.utsouthwestern.edu Sent: Thu, December 17, 2009 6:15:17 AM Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has a

[Histonet] Silver Nitrate instead of inking??

2009-12-17 Thread sheila adey
Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _ E

Re: [Histonet] Silver Nitrate

2009-07-08 Thread John Kiernan
-- From: Andrea Grantham Date: Wednesday, July 8, 2009 12:00 Subject: [Histonet] Silver Nitrate To: HISTONET > After a recent run on silver stains here I need to order > Silver > Nitrate. Can anyone tell me what the difference is (except the > cost) > between Silver Nitrate >

[Histonet] Silver Nitrate

2009-07-08 Thread Andrea Grantham
After a recent run on silver stains here I need to order Silver Nitrate. Can anyone tell me what the difference is (except the cost) between Silver Nitrate Certified ACS, USP, p.a., Extra Pure, and Ultra Pure? They all have the same chemical formula and FW of 169.87. My last batch of Silver

[Histonet] Silver staining /other methods to reveal axonsprouting/retraction following injury

2009-06-25 Thread TF
Hi all i read such figures in Cajal's books..I guess he is not using specific degenerative sliver staining, as Natua developed the method 50 years later. I wonder anyone can send me the protocol for such kind of silver staining - there are so many ways of Golgi staining - to reveal post-injury c

[Histonet] Silver staining /other methods to reveal axon sprouting/retraction following injury

2009-06-24 Thread TF
Hi all i read such figures in Cajal's books..I guess he is not using specific degenerative sliver staining, as Natua developed the method 50 years later. I wonder anyone can send me the protocol for such kind of silver staining - there are so many ways of Golgi staining - to reveal post-injury c

RE: [Histonet] silver stain for mouse intestinal nerves

2009-01-12 Thread Smith, Allen
: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Monday, January 12, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain for mouse intestinal nerves I am looking for a silver stain method that would

[Histonet] silver stain for mouse intestinal nerves

2009-01-12 Thread Helen E Johnson
I am looking for a silver stain method that would stain nerves in intestines of mice. Helen Johnson (he...@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged o

RE: [Histonet] Silver staing for degenerating axons, thank you for sharing the paper.

2008-12-16 Thread Stapf, Ross
A great thank you to the person who shared this paper. This was a very interesting read. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax ro...@baylorhealth.edu

[Histonet] Silver staing for degenerating axons

2008-12-15 Thread tf
I read the paper of Nauta with great interest! But I dont have the original pdf for the paper on Stain Technology in 1951 & 1954. Anyone can send me the pdf? I only have the improved method by Fink and Heimer in 1967 published on brain research, giving out two staining procedures. I can send yo

[Histonet] Silver staing for degenerating axons

2008-12-15 Thread tf
I read the paper of Nauta with great interest! But I dont have the original pdf for the paper on Stain Technology in 1951 & 1954. Anyone can send me the pdf? I only have the improved method by Fink and Heimer in 1967 published on brain research, giving out two staining procedures. I can send yo