Cindy McGrady at Hurley Medical Center in Flint, Michigan asks:
>>Our laboratory is experimenting with xylene free processing on our Sakura
VIP, substituting isopropanol for our xylene substitute. Our pathologists
have complained about processing, especially the biopsies, with our regular
Our laboratory is experimenting with xylene free processing on our Sakura
VIP. , substituting Isopropanol for our xylene substitute. Our Pathologists
have complained about processing, especially the biopsies, with our regular
processing, they have the washed out look, nuclear staining is faint.
If
I am also interested in that i run a small lab and would love to go xylene
free!
2017-02-23 12:21 GMT-06:00 Blake Taylor via Histonet <
histonet@lists.utsouthwestern.edu>:
> Our hospital has asked us to investigate going xylene free. Has anyone
> recently made a switch to a xylene alternative
Our hospital has asked us to investigate going xylene free. Has anyone
recently made a switch to a xylene alternative and have you been happy with
your results? We currently have 4 VIP processors and two Leica auto stainers.
Thanks for your input.
Blake Taylor
Surgical Pathology Supervisor
Finlay brendal.fin...@medicalcenterclinic.com,
histonet@lists.utsouthwestern.edu
Date: 08/13/2015 15:33
Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section
Questions
1-After you oven dry your stained sections, you use the very same medium you
always have used. I used
Finlay, HT (ASCP)
-Original Message-
From: Rene J Buesa rjbu...@yahoo.com
To: Brendal Finlay brendal.fin...@medicalcenterclinic.com,
histonet@lists.utsouthwestern.edu
Date: 08/13/2015 15:33
Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section
Questions
1-After you
For those labs who are xylene free I have two questions:
1. What mounting medium do you use when coverslipping oven dried slides?
2. How are you running down and coverslipping frozen sections after staining
them?
I'd like to make sure the stains are preserved over time and my experience
1-After you oven dry your stained sections, you use the very same medium you
always have used. I used Permount.2- Given the very special constrains of FS
(ready for diagnoses within 20 minutes of receiving the specimen) I used an
aqueous mounting medium. After the diagnosis was made, I
Is there anyone willing to share their processing protocol for using
isopropanol without xylene?
Justin Peters, HTL, QIHC (ASCP)CM
Histology Supervisor
Henrico Doctors' Hospital - Forest, Parham, and Retreat Campuses
___
Histonet mailing list
Hello,
How common are xylene-free labs in the histo world? I worked for 3 years at a
research service histology lab, and am now in a research lab. I miss
histology, and have considered getting certified so that I can have a career in
it, but my sensitivity to xylene has made me hesitate. Is
Hi,
We have been processing xylene free for a couple of years in ThermoFisher
Excelsior processors. The tissue goes directly from isopropyl alcohol to
paraffin. It works well for us. We sought out a xylene free protocol to
reduce the amount of xylene in the lab.
We do need to use
To: histonet histonet@lists.utsouthwestern.edu
Sent: Thursday, January 10, 2013 1:13 PM
Subject: [Histonet] Xylene free processing
Hi,
We have been processing xylene free for a couple of years in ThermoFisher
Excelsior processors. The tissue goes directly from isopropyl alcohol to
paraffin. It works
I would appreciate hearing all of your expert opinions on processing tissue
without the use of xylene or any type of xylene substitute. Our processor was
down recently, and a friendly local lab processed our tissue this way for us.
Of course, the Pathologists loved it. The process was
@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Free Processing?
I would appreciate hearing all of your expert opinions on processing
tissue without the use of xylene or any type of xylene substitute. Our
processor was down recently, and a friendly local lab processed our
tissue this way for us. Of course
Please go to http://www.histosearch.com/rene.html and you will find my articles
on the sibject.
René J.
From: Jones, Laura lpjo...@srhs-pa.org
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Sent: Wednesday, January 9, 2013 2:37 PM
Subject: [Histonet] Xylene Free
René J.
From: O'Donnell, Bill billodonn...@catholichealth.net
To: Jones, Laura lpjo...@srhs-pa.org; Histonet@lists.utsouthwestern.edu
Sent: Wednesday, January 9, 2013 3:15 PM
Subject: RE: [Histonet] Xylene Free Processing?
The Xpress microwave tx processors are xylene free - perhaps they have
one
] On Behalf Of Rene J Buesa
Sent: Wednesday, January 09, 2013 2:46 PM
To: Jones, Laura; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene Free Processing?
Please go to http://www.histosearch.com/rene.html and you will find my articles
on the sibject.
René J.
From: Jones, Laura lpjo...@srhs
Sent: January 9, 2013 12:16 PM
To: Jones, Laura; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene Free Processing?
The Xpress microwave tx processors are xylene free - perhaps they have
one.
I am pretty certain there still needs to be something that is misable
with alcohol
Hello,
I have been processing our tissue (derm only) in our Shandon Excelsior using a
xylene free process with Iso Alcohol. A few years back it was set up that way
by a Thermo rep and it worked really well. I am now having issues and the docs
seem to think it is a processing issue rather than
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