Re: [Histonet] Oil Red O

Hello John Thank you very much for the great source of information! Maki From: John Kiernan Sent: Saturday, March 6, 2021 9:55 AM To: histonet@lists.utsouthwestern.edu ; Niihori, Maki - (mniihori) Subject: [EXT]Re: Oil Red O External Email It's in any

Re: [Histonet] Oil Red O

In Histological and Histochemical Methods in Theory and Practice by John Kiernan there is a good method for Oil Red O. There is definitely an art to a good Oil Red O that is passed down from the older experienced tech to the young folks in the lab. Bancroft’s Theory and Practice of

Re: [Histonet] Oil Red O

It's in any textbook in the field of histotechnology published since about 1950. This one from Amazon costs less than $8. Every lab should have a shelf of such books. https://www.amazon.com/Introduction-Histotechnology-Geoffrey-G-Brown/dp/0838543405

Re: [Histonet] Oil Red O

What have you fixed the tissue with? Can you cut frozen sections from the fixed tissue? This is useful information to start with. John On Fri, Mar 5, 2021 at 1:08 PM, Niihori, Maki - \(mniihori\) via Histonet wrote: > We would like to stain Right Ventricle (RV) and Lung (both from rat

Re: [Histonet] Oil Red O permanent mountant

UNCLASSIFIED Hi again, Meant to say ThermoFisher Aqua Mount, not Permount in my previous post. Apologies, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au Phone (02) 5124 2874

Re: [Histonet] Oil Red O

Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too. mills ᐧ On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia

RE: [Histonet] Oil red O

: Jennifer MacDonald CC: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; P.E. Visser Onderwerp: Re: [Histonet] Oil red O It is for Sudan Red 5B. All these apothecary names are essentially meaningless René J. From: Jennifer MacDonald mailto:jmacdon...@mtsac.edu

Re: [Histonet] Oil red O

Rene, You still have not answered the question - what does the O stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724

RE: [Histonet] Oil red O

:06 PM Cc: HISTONET Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the O stand for? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ

Re: [Histonet] Oil red O

As you are finding out the answers to dye nomenclature you can had these endings to your list of the O' in Oil red O: Oil red EGN Oil red 4B Ada Feldman Anatech Ltd. 1020 Harts Lake Road Battle Creek, MI 49037 Phone: 800.262.8324 Phone: 269.964.6450 Fax: 269.964.8084

Re: [Histonet] Oil red O

: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: Cc: HISTONET histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:06 PM Subject: Re: [Histonet] Oil red O Rene, You still have not answered the question - what does the O stand for? Andrea Grantham, HT (ASCP) Senior

Re: [Histonet] Oil red O

@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 3:49 PM Subject: RE: [Histonet] Oil red O I believe the question is:  what does the O in Oil Red O stand for? Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI

RE: [Histonet] Oil red O

I'm not sure, but whatever you do... don't store it next to the Sudan Black B...you'll stink up the lab!! Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803

Re: [Histonet] Oil red O

. Visser p...@xs4all.nl Sent: Monday, September 9, 2013 3:53 PM Subject: Re: [Histonet] Oil red O I believe the question is what is the O signify. as in OG6 the O is for orange. From:  Rene J Buesa rjbu...@yahoo.com To:    P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu histonet

Re: [Histonet] Oil red O

There you have it! René J. From: Ada Feldman adafeld...@anatechltdusa.com To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:13 PM Subject: Re: [Histonet] Oil red O As you are finding out the answers to dye nomenclature you can had

RE: [Histonet] Oil red O

...@anatechltdusa.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil red O CC: There you have it! René J. From: Ada Feldman adafeld...@anatechltdusa.com To: histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:13 PM Subject: Re

RE: [Histonet] Oil red O

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, September 09, 2013 2:00 PM To: Rene J Buesa; Ada Feldman; histonet Subject: RE: [Histonet] Oil red O I believe the answer should be - Oil Red O is a brand name (not uncommon

Re: [Histonet] Oil red O

To: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Cc: HISTONET histonet@lists.utsouthwestern.edu Sent: Monday, September 9, 2013 4:11 PM Subject: RE: [Histonet] Oil red O May be the position of the active group on the benzene ring - ortho vs. meta vs. para ? -Original Message- From

RE: [Histonet] Oil red O

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, September 09, 2013 2:45 PM To: P.E. Visser; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil red O Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I

Re: [Histonet] Oil red O

I believe the question is what is the O signify. as in OG6 the O is for orange. From: Rene J Buesa rjbu...@yahoo.com To: P.E. Visser p...@xs4all.nl, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 09/09/2013 12:46 PM Subject:Re: [Histonet] Oil red O

Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain? It is described in any technology book. I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO René J. From: P.E. Visser p...@xs4all.nl To:

Re: RE: [Histonet] Oil red O

Feldman; histonet Subject: RE: [Histonet] Oil red O I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B William DeSalvo, BS HTL(ASCP) Date: Mon, 9 Sep 2013 13:32:08

RE: [Histonet] Oil red O stain on formalin fixed liver

David, (From Doug Reye's old Laboratory). I have found the following technique the best: Cryotomy of Formalin Fixed Tissues Sometimes frozen sections are requested on tissue that has been fixed in 10% formalin. Cutting frozen sections of formalin fixed tissue can be frustrating at times due

RE: [Histonet] Oil Red O

Oil Red O slides will never fade. The technique isn't like typical histochemical procedures where dye molecules bind chemically to tissue components. In Oil Red O staining there isn't any chemical binding that can be reversed. What you end up with is a solution of the dye in the fat droplets.

RE: [Histonet] Oil red O versus Sudan 4

Candice, Remember freshly prepared Oil Red O Stock usually does not work. Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager

Re: [Histonet] Oil red O versus Sudan 4

...@mirnarx.com To: Candice Smoots candice_cami...@yahoo.com Sent: Wednesday, January 25, 2012 10:32 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 I do oil red a lot.  You are doing it on frozen sections right? What are you coverslipping with? Need a few more details of the protocol and I

Re: [Histonet] Oil red O versus Sudan 4

: RE: [Histonet] Oil red O versus Sudan 4 Candice Most of the references regarding this technique use Sudan IV, that's what we use here. We use Oil Red O for the Aortic Root Sections but not for the En Face Analysis of the Aorta.  I have the Sudan IV procedure we use here if you would like

RE: [Histonet] Oil Red O for FS on Muscle

Hi, We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the whole bottle before we use it and then we apply it to unfixed slides for about 30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip with Aquamount. We use it for cytospins and occasionally

RE: [Histonet] Oil Red O for FS on Muscle

Allison We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum. That seems to help a bit with the background and we are working

Re: [Histonet] Oil Red O for FS on Muscle

I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it

Re: [Histonet] Oil Red O

That's the way it normally looks, kinda globby. ORO is really not that soluble in fat, just more soluble than the prop. glycol. Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list

Re: [Histonet] Oil Red O trivia

So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange

Re: [Histonet] Oil Red O control

There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org

RE: [Histonet] Oil Red O control

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives

RE: [Histonet] Oil Red O control

Once you mix your stock solution with water, it is good for only about an hour. Then you have to discard it. Michelle Aloni MS HTL (ASCP) USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Oil Red O control

Cut frozen section of adrenal from an autopsy or surgical pathology. Store slides in a box in the refrig. Good for a year. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Inman, Anna anna.in...@stmarygj.org Sent:

RE: [Histonet] Oil red o mounting media

We use Crystalmount. You don't even have to coverslip. Just make sure you evenly distribute the mountant on the slide and let it dry. We usually put it on a hot plate for a bit. This leaves a hardened shell around the tissue. The slides can be read like this too. If you really need a coverslip,

Re: [Histonet] Oil Red O despair - point of clarification

I didn't mean that the propylene glycol and glycerin jelly were combined - just wanted to clear that up. The protocol uses both but for different things. Andi Grantham At 09:12 AM 11/18/2008, Andrea Grantham wrote: I get many projects here that require frozen liver sections and Oil Red O