I use this system quite often, when doing manual IHC. I believe that these
are air bubbles that were in the buffer solution that you used when you first
put the slides into the cytoclips; I usually let the buffer settle a little bit
in the beaker before I load my slides (to allow time for any b
Hi
Looking at the images this appears to be the prozone effect which is caused by
the primary antibody concentration being too high and has been well reported in
both immunohistochemistry, immunology and serology articles. Do a google serch
for prozone phenomenon and you will find an explanat
It looks like bubble trapped between the slide and coverplate. Are you using
sufficient surfactant? That can help, but any of the "cap gap" methodologies
lend themselves to this problem.
Rinse your slides very thoroughly in buffer with plenty of surfactant before
loading them on the instrument
Those appear in all 14 slides I stained - I doubt to have made the same
mistake of leaving bubbles fourteen times when mounting them.
That my friend is the result of a air bubble on the section. If it had
been unremoved xylene the hematoxylin would have been absent as well.
I have seen some
I have seen something (rarely) like this. We always assume that a few
bubbles developed that impeded the flow of one or more critical reagents
to the tissue underlying the bubble. Somehow, the impediment vanished
later in staining to allow for the Hematoxylin counterstain to take. It
would only
Hi V.
That my friend is the result of a air bubble on the section. If it had
been unremoved xylene the hematoxylin would have been absent as well.
Cheers.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlotteto
