We still run negative controls.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I just wanted to clarify there are two types of negative controls, one is a
negative reagent control and one is a tissue that is known negative. A lot of
time, we tend to focus on the negative reagent control and forget about the
negative tissue control. I know that a lot of smart folks out
This is what I put in my procedure shortly after Richard sent his email. There
wasn't a problem at inspection.
Following his announcement at NSH/ISH Forum in Windsor, CT, Dr. Richard
Carten sent an email to Histonet on July 17, 2102, regarding CAP guidelines for
negative controls. The
Negative controls for IHC has been discussed before many times but it keeps
pupping up every noew and then.
This is my take on it:
1- if CAP requires them, so then do it. You do not want to have a negative
issue in your inspection
2- it is always good to have them for quality control of your
We have eliminated them all per the CAP guidelines.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint
Yes you can, the cap standards just came out Tuesday with the revised part
stating you do not have to run a negative reagent control using polymer based
detection. Only if you are still using biotin based detection kits do you need
to run the neg. control
Vanessa Perez Garcia
Histology
, August 02, 2012 8:35 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] negative controls
Yes you can, the cap standards just came out Tuesday with the revised
part stating you do not have to run a negative reagent control using
polymer based detection. Only if you
, August 02, 2012 8:35 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] negative controls
Yes you can, the cap standards just came out Tuesday with the revised
part stating you do not have to run a negative reagent control using
polymer based detection. Only if you
I asked my VMS rep that question and she forwarded it to the higher ups
as they had not heard about this yet. Waiting for an answer but even if
VMS says we can omit them, our negative aren't all that clean always so
don't know if we will or not.
-Original Message-
From:
Hi Chris,
CAP actually has a specific checklist question on Negative Controls.
The question is ANP.22570 and if
I remember correctly it is quite extensive. Most of the CAP questions are
about 7 to 10 lines. The Negative control question is more than a page long.
Get your hands a copy of the most
, 2011 10:42 AM
Subject: RE: [Histonet] negative controls on immunos
Fun no, impractical yes.
Weems, Joyce jwe...@sjha.org 3/2/2011 10:30 AM
The CAP guidelines are pretty clear. Copied from latest checklist..
Isn't this fun???! j:)
ANP.22570 Phase IIN/A YES
Yes, it is always best to run every conceivable control available. Then you can
be really, really, really sure. However, if you have practical issues that come
into play such as cost of reagents, or extra controls taking up valuable space
on the stainer then you might have to think about what
] On Behalf Of Greg Dobbin
Sent: Wednesday, March 02, 2011 09:15
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then you can
be really, really, really sure. However, if you
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then
you can be really, really, really sure. However, if you have practical
issues that come into play such as cost of reagents, or extra controls
taking up valuable space
...@lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Wednesday, March 02, 2011 09:15
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] negative controls on immunos
Yes, it is always best to run every conceivable control available. Then
you can be really, really, really sure
, October 15, 2010 12:50 PM
To: Victoria Baker; Histo Net list server
Subject: RE: [Histonet] negative controls
Hi Vikki,
I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to
each ultra. This means not all slides from any case are guaranteed to be on
the same machine
To my knowledge for this stain to be legitimate and meet criteria you must
run your positive and negative controls on the same run.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
Pretty sure you have to run your positive with your negative to keep the
conditions 100% the same. You don't have to put them on the same slide
necessarily, but they need to be on the same run. Also, say you are
running 15 HP patient slides, you can have one negative control for all
of these as
Hi Vikki,
It's best to run it all together and run a negative control for each
detection kit.
Mark
On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker bakevicto...@gmail.comwrote:
Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with
We run negative controls on every block of a case within the same run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative
(ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
=
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri
...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa rjbu...@yahoo.com
Date: Fri, October 15, 2010 8:33 am
, you could go pull the control for that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J
Subject
RE: [Histonet] negative controls
Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com]
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls
So for every HP you do, you process
To: sgoe...@xbiotech.com
Cc: Histo Net list server; histonet-boun...@lists.utsouthwestern.edu; Sebree
Linda A; Rene J Buesa
Subject: RE: [Histonet] negative controls
We're talking NEGATIVE controls here folks and if you use internal tissue
for your positive and negative controls
Hi Vikki,
I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated
to each ultra. This means not all slides from any case are guaranteed
to be on the same machine. They obviously all have their own detection
kit. My theory is that our detection kits are QCd prior to initial load
Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com]
Sent: Friday, October 15, 2010 10:48 AM
To: Rene J Buesa
Cc: Histo Net list server; Sebree Linda A
Subject: RE: [Histonet] negative controls
So for every HP you do, you
] On Behalf Of Drew Sally A
Sent: 04 March 2010 16:02
To: Histonet
Subject: RE: [Histonet] Negative controls
Yes, Linda and I select the harshest protocol in the list of antibodies
we're running per case-we figure that the harshest will produce the
biggest problems with background, etc.
Sally Ann
We too use the Universal negative from Biocare, and it has passed
through 2 CAP inspections so no problem on that front.
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From:
Yes, Linda and I select the harshest protocol in the list of antibodies
we're running per case-we figure that the harshest will produce the
biggest problems with background, etc.
Sally Ann Drew, MT(ASCP)
IHC/ISH Clinical Research Laboratory
UWHC
600 Highland Ave. DB1-223, Mail Code 3224
Madison,
Anita,
If you're referring to the reagents you use, we use a Universal Negative
Control Serum (Biocare) on our negative control slides. We have 3
Ventana instruments and have never been cited.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland
I don't see how negative controls for ordinary histochemical procedures would
be much help unless they are the same tissue as the test sample. If you are
doing a congo red for amyloid plaques in brain, I can see using a known
negative brain as a control. But I don't see the point of running a
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