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-Original Message-
From: Geoff McAuliffe [mailto:mcaul...@umdnj.edu]
Sent: Wednesday, December 02, 2009 1:41 PM
To: Liz Chlipala
Cc: histo...@pathology.swmed.edu
Subject: Re: [Histonet
Re: [Histonet] procedure for osmium and potassium chromate?
To: histo...@pathology.swmed.edu, Liz Chlipala
Message-ID: <405109.33036...@web65705.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I really would suggest you to find an alternative procedure.
Osmium tetroxyde is EX
I really would suggest you to find an alternative procedure.
Osmium tetroxyde is EXTREMELY dangerous and has to be used under extreme
precautions.
I don't think the results are worth the effort given the great dangers it poses.
I for one would not share the procedure with you.
René J.
--- On Wed,
OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS)
Solutions
2.5% Potassium Dichromate Solution Stock
1% Osmium Tetroxide Solution Stock
Potassium Dichromate-Osmium Tetroxide Solution Working
Potassium Dichromate Stock 50 ml
Osmium Tetroxide stock 50 ml
Staining Procedure
1. Place formalin f
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-Original Message-
From: Geoff McAuliffe [mailto:mcaul...@umdnj.edu]
Sent: Wednesday, December 02, 2009 1:41 PM
To: Liz Chlipala
Cc: histo...@pathology.swmed.edu
Subject: Re: [Histonet] procedure for osmium and potassium
Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with osmium, alone or mixed with something else. Is this
for EM or LM?
