Hello Guys
I have a trivial query, I have a protein stock 1000 µg per ml. Now I would
like to load on each well 0.25, 0.5, 1, 2, 4, 8, 16 and 32 µgs.
For 0.25 µg, 0.25 µl from stock + 0.25 µl 2x sample buffer , total 0.5 µl
load on first lane. If I want to load higher amount like 16 µg and 32 µgs
Hi Sudheer,
Your protein stock is 1000 ug/mL = 1 ug/ul. That means you will need 32 ul
protein stock to get your 32 ug sample. If the maximum volume you can load in
your well is 16 ug, then you will not be able to prepare an appropriate 32 ug
sample from the stock you have now. You can concentr
In article ,
sudheer.pb...@gmail.com says...
>
> Hello Guys
> I have a trivial query, I have a protein stock 1000 µg per ml. Now I would
> like to load on each well 0.25, 0.5, 1, 2, 4, 8, 16 and 32 µgs.
> For 0.25 µg, 0.25 µl from stock + 0.25 µl 2x sample buffer , total 0.5 µl
> load on first
