I thought I did. Here's what I wrote regarding XPRESS: It simply reports
light area to heavy area ratios. You'll have to do adjustments after the
fact i.e. centering the mean ratio to 1:1 if you expect the bulk of your
samples to be 1:1.
If that doesn't answer your normalization question,
Hi Jimmy,
Can you please explain my query.
Regards,
Sud
On Wed, Nov 22, 2023 at 3:13 PM sudarshan kumar
wrote:
> Oh ! thank you
> Let me explain-
> I have mixed two samples light and heavy labelled proteins (at cell level)
> But if the cell count was not equal in the two groups, it will result
Oh ! thank you
Let me explain-
I have mixed two samples light and heavy labelled proteins (at cell level)
But if the cell count was not equal in the two groups, it will result in
unequal contribution of proteins to the mixture. In this case the ratio L/H
for individual protein will not be correct.
I don't know what "normalized intensity/peak area" of each peptide means in
this context but I can say that XPRESS doesn't do any normalization. It
simply reports light area to heavy area ratios. You'll have to do
adjustments after the fact i.e. centering the mean ratio to 1:1 if you
expect
