I'm trying to run Protein prophet but keep running into the same
error.
So far, I started with a .RAW file (290MB) and converted it to both
mzXML (667Mb) and mzML (1GB). I then did a Database search and got
the
expected folder with .dta files (2852 files, 1-15KB each). I
converted these to a .pep.xml file (3KB). The problem comes when I
try to analyze with Peptide Prophet and Protein Prophet. I get the
following files: interact.pep.xml,
interact.pep.shtml,interact.pep.xsl, and interact.pep.xml.tmp.axxxxx.
I then get the following error messages:
--------------------------------------
Popup message:
PeptideProphetParser.exe has encountered a problem and needs to
close. We are sorry for the inconvenience.
--------------------------------------
Error Signature:
szAppName : PeptideProphetParser.exe szAppVer : 0.0.0.0
szModName : PeptideProphetParser.exe szModVer : 0.0.0.0
offset : 00019ec0
--------------------------------------
Error Report Contents:
C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp
\WERb712.dir00\PeptideProphetParser.exe.mdmp
C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp\WERb712.dir00\appcompat.txt
--------------------------------------
Log File/ Output Files:
* c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
interact.pep.shtml [ View ]
* c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
interact.prot.shtml (file not found: not created or deleted?)
Command 1 [Tue Dec 9 09:33:43 2008] [ Show / Hide ]
run_in c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test;
xinteract -Ninteract.pep.xml -p0.05 -x20 -Op c:/Inetpub/wwwroot/ISB/
data/18337.RAW_analysis_test/18337.pep.xml
xinteract (TPP v4.0 JETSTREAM rev 2, Build 200807011544 (MinGW))
running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/18337.pep.xml""
file 1: c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
18337.pep.xml
processed altogether 0 results
results written to file c:/Inetpub/wwwroot/ISB/data/
18337.RAW_analysis_test/interact.pep.xml
direct your browser to
http://localhost/ISB/data/18337.RAW_analysis_test/interact.pep.shtml
command completed in 1 sec
running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20"
(SEQUEST)
MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN,
Ionization:
UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN
command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20" failed: Unknown error
command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
MINPROB=0.05 EXTRAITRS=20" exited with non-zero exit code:
-1073741819
QUIT - the job is incomplete
Command FAILED
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