Hello Amanda,

Please try the latest release, 4.1.1 (November) instead of 4.0.2 (July), as
many bugs have been fixed.  Perhaps this issue has already been addressed.

Best wishes,
Natalie


On Mon, Dec 15, 2008 at 2:51 PM, Amanda <[email protected]> wrote:

>
> I'm trying to run Protein prophet but keep running into the same
> error.
> So far, I started with a .RAW file (290MB) and converted it to both
> mzXML (667Mb) and mzML (1GB). I then did a Database search and got
> the
> expected folder with .dta files (2852 files, 1-15KB each).  I
> converted these to a .pep.xml file (3KB).  The problem comes when I
> try to analyze with Peptide Prophet and Protein Prophet. I get the
> following files: interact.pep.xml,
> interact.pep.shtml,interact.pep.xsl, and interact.pep.xml.tmp.axxxxx.
> I then get the following error messages:
> --------------------------------------
> Popup message:
> PeptideProphetParser.exe has encountered a problem and needs to
> close.  We are sorry for the inconvenience.
> --------------------------------------
> Error Signature:
> szAppName : PeptideProphetParser.exe     szAppVer : 0.0.0.0
> szModName : PeptideProphetParser.exe     szModVer : 0.0.0.0
> offset : 00019ec0
> --------------------------------------
> Error Report Contents:
> C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp
> \WERb712.dir00\PeptideProphetParser.exe.mdmp
> C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp\WERb712.dir00\appcompat.txt
> --------------------------------------
> Log File/ Output Files:
>
>    * c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
> interact.pep.shtml [ View ]
>    * c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
> interact.prot.shtml (file not found: not created or deleted?)
>
>
>  Command 1 [Tue Dec 9 09:33:43 2008]               [ Show / Hide ]
>
>
>  run_in c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test;
> xinteract  -Ninteract.pep.xml -p0.05 -x20 -Op c:/Inetpub/wwwroot/ISB/
> data/18337.RAW_analysis_test/18337.pep.xml
>
>
> xinteract (TPP v4.0 JETSTREAM rev 2, Build 200807011544 (MinGW))
>
>
> running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
> Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/18337.pep.xml""
>  file 1: c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
> 18337.pep.xml
>  processed altogether 0 results
>
>
>  results written to file c:/Inetpub/wwwroot/ISB/data/
> 18337.RAW_analysis_test/interact.pep.xml
>
>
>  direct your browser to
> http://localhost/ISB/data/18337.RAW_analysis_test/interact.pep.shtml
>
>
> command completed in 1 sec
>
>
> running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
> MINPROB=0.05 EXTRAITRS=20"
>  (SEQUEST)
> MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN,
> Ionization:
> UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN
>
>
> command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
> MINPROB=0.05 EXTRAITRS=20" failed: Unknown error
>
>
> command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
> MINPROB=0.05 EXTRAITRS=20" exited with non-zero exit code:
> -1073741819
> QUIT - the job is incomplete
>
>
> Command FAILED
>
>
>
> >
>

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