Hi Amanda,

The error message says:

running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/18337.pep.xml""
 file 1: c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
18337.pep.xml
 processed altogether 0 results

This means there was a problem reading data from your pepXML file.
Can you verify that your pepXML fie has spetrum_query tags?

Thanks,
-David

On Mon, Dec 15, 2008 at 2:59 PM, Natalie Tasman
<[email protected]> wrote:
> Hello Amanda,
>
> Please try the latest release, 4.1.1 (November) instead of 4.0.2 (July), as
> many bugs have been fixed.  Perhaps this issue has already been addressed.
>
> Best wishes,
> Natalie
>
>
> On Mon, Dec 15, 2008 at 2:51 PM, Amanda <[email protected]> wrote:
>>
>> I'm trying to run Protein prophet but keep running into the same
>> error.
>> So far, I started with a .RAW file (290MB) and converted it to both
>> mzXML (667Mb) and mzML (1GB). I then did a Database search and got
>> the
>> expected folder with .dta files (2852 files, 1-15KB each).  I
>> converted these to a .pep.xml file (3KB).  The problem comes when I
>> try to analyze with Peptide Prophet and Protein Prophet. I get the
>> following files: interact.pep.xml,
>> interact.pep.shtml,interact.pep.xsl, and interact.pep.xml.tmp.axxxxx.
>> I then get the following error messages:
>> --------------------------------------
>> Popup message:
>> PeptideProphetParser.exe has encountered a problem and needs to
>> close.  We are sorry for the inconvenience.
>> --------------------------------------
>> Error Signature:
>> szAppName : PeptideProphetParser.exe     szAppVer : 0.0.0.0
>> szModName : PeptideProphetParser.exe     szModVer : 0.0.0.0
>> offset : 00019ec0
>> --------------------------------------
>> Error Report Contents:
>> C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp
>> \WERb712.dir00\PeptideProphetParser.exe.mdmp
>> C:\DOCUME~1\AMANDA~1\LOCALS~1\Temp\WERb712.dir00\appcompat.txt
>> --------------------------------------
>> Log File/ Output Files:
>>
>>    * c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
>> interact.pep.shtml [ View ]
>>    * c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
>> interact.prot.shtml (file not found: not created or deleted?)
>>
>>
>>  Command 1 [Tue Dec 9 09:33:43 2008]               [ Show / Hide ]
>>
>>
>>  run_in c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test;
>> xinteract  -Ninteract.pep.xml -p0.05 -x20 -Op c:/Inetpub/wwwroot/ISB/
>> data/18337.RAW_analysis_test/18337.pep.xml
>>
>>
>> xinteract (TPP v4.0 JETSTREAM rev 2, Build 200807011544 (MinGW))
>>
>>
>> running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/
>> Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/18337.pep.xml""
>>  file 1: c:/Inetpub/wwwroot/ISB/data/18337.RAW_analysis_test/
>> 18337.pep.xml
>>  processed altogether 0 results
>>
>>
>>  results written to file c:/Inetpub/wwwroot/ISB/data/
>> 18337.RAW_analysis_test/interact.pep.xml
>>
>>
>>  direct your browser to
>> http://localhost/ISB/data/18337.RAW_analysis_test/interact.pep.shtml
>>
>>
>> command completed in 1 sec
>>
>>
>> running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
>> MINPROB=0.05 EXTRAITRS=20"
>>  (SEQUEST)
>> MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN,
>> Ionization:
>> UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN
>>
>>
>> command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
>> MINPROB=0.05 EXTRAITRS=20" failed: Unknown error
>>
>>
>> command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml"
>> MINPROB=0.05 EXTRAITRS=20" exited with non-zero exit code:
>> -1073741819
>> QUIT - the job is incomplete
>>
>>
>> Command FAILED
>>
>>
>>
>>
>
>
> >
>

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