Dear ： Hi,
There is some question when I use the Xpress from Trans-Proteomic Pipeline for analysis and validation of peptides and proteins ,which was labeled by SILAC, after searching the raw files through sequest( Bioworks Browser ,Thermo Fisher, San Jose, CA) against the NCBI RefSeq database of human sequences. For the instrument we used was a LC-MS/MS（LTQ,Thermo),the sensitivity of which is no good than that of LTQ-FT or LTQ-Orbitrap.How to set the ‘ Change Xpress mass tolerance' in the Xpress Options ? And what's the differense between it and the ' peptide tolerance'and 'Fragment ions tolerance' in the Sequest Search Parameters? We set the peptide tolerance as 2.0 amu and Fragment ions tolerance as 1.0 amu , are they reasonable with regard to our LTQ ? Please return as soon as posssible. All yours -- Yulu Yang Ph.D Candidate School of Life Science and Biotechnology Shanghai Jiao Tong University P.R. China 86-21-34204875(office) -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to spctools-discuss@googlegroups.com. To unsubscribe from this group, send email to spctools-discuss+unsubscr...@googlegroups.com. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
